rev:      October 1, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

IL-8 Receptor (IL-8R)- ANTISERA see polyclonals and monoclonals below

DATA SHEET: mouse anti-human IL8RB   (Interleukin 8 Receptor Beta Chain) CXCR2

Catalog#: RDI-IL8RB-483     $535.00/vial

Package Size: 500ug purified with 5% trehalose. Reconstitute with sterile PBS.

Species: mouse IgG2A CLONE: Activity: Reacts with human interleukin 8 receptor Beta (IL- 8RB) Does not cross react with CXCR1 (IL-8RA)

Application: Flow cytometry, Immunohistochemistry, and Neutralization

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-IL8RA-5A12    $531.00/vial - DISCONTINUED

FITC labeled (with azide) cat#RDI-IL8RA-5AFt $625.00/0.2mg  (Biotin & PE on request)

    * no azide formulation cat#RDI-IL8RA-5A12X      $781.00/vial - DISCONTINUED

Package Size: 500ug purified with 0.1% sodium azide*. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)

Prep: purified from tissue culture supernatant via Protein G affinity chromatography.

Species: mouse IgG2b

CLONE: 5A12

Activity: Reacts with human interleukin 8 receptor alpha (IL- 8RA), a G protein linked seven transmembrane domain protein, expressed on monocytes and granulocytes. It is also expressed on a subset of T cells. IL-8RA and IL-8RB are high affinity receptors for IL-8.

Application: -Indirect immunofluorescence cell surface staining

                   -blocking: NOTE: Use azide free formulation for blocking. It has been found that the anti-IL8Ra antibody cat#RDI-IL8RA-5A12 blocks IL8 mediated chemotaxis more effectively than anti-IL-8RB. A combination of both is recommended for blocking chemotactic activity. A concentration of 0.8- 15ug/ml each of anti-IL8RA and anti-IL8RB can block by 50% the chemotaxis of human neutrophil induced by 50ng/ml rhIL-8.

Titer for each application

References: 1) Eur J. immunol. 26:640

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humansor animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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