rev: February 11, 2005
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
INTERFERON GAMMA Receptor ANTISERA
-see also new rec human interferon gamma soluble receptor antigen
and anti-mouse CD119
CHAIN 1 (MMHGR-1)
CAT# RDI-PB21580 $375.00/vial
Quantity Shipped: 1 vial of 200ug in 200 ul
Concentration: 1.0 mg/ml.
Buffer: Phosphate buffered saline (PBS)
Isotype: to be determined
Bioactivity: Binds to but does not neutralize human interferon gamma
receptor.
Usage: Western blot; immunoprecipitation; immunohistochemistry; flow cytometry.
Shipping Temperature: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-20C or below for retention of full activity. When thawing, the contents
of the tube should be apportioned in separate tubes so that freezing and
thawing is kept to a minimum.
-For In Vitro Research Use Only-
Description: Rabbit Anti-Human Interferon Gamma Receptor Chain 1,non-neutralizing
Quantity Shipped: 1 vial of 200 ug in 200 ul.
Concentration: 1.0 mg/ml.
Bioactivity: Binds to but does not neutralize human interferon gamma
receptor
Usage: Western blot (1:3000 dilution); immunoprecipitation (~1 ul per 1 x
10 (7) cells); immunohistochemistry; flow cytometry (1:1000 dilution).
Shipping Temperature: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-20'C or below for retention of full activity. When thawing, the contents
of the tube should be apportioned in separate tubes so that freezing and
thawing is kept to a minimum.
-For In Vitro Research Use Only-
CHAIN 2 (MMHGR-2) =Beta chain Non Neutralizing
CAT# RDI-PB21585 $438.00/vial
Quantity Shipped: 1 vial of 250 ug in 0.1ml
Concentration: 2,5mg/ml
Clone ref: MMHGR-2
Buffer: Phosphate buffered saline (PBS) with 0.1% BSA
Isotype: IgG1
Antigen: human interferon gamma receptor chain 2
Bioactivity: Binds to but does not neutralize human interferon gamma receptor.
Binds to extracellular region of the receptor.
Usage: Western blot (1:2000 dilution); immuno- precipitation (1:100);
immunohistochemistry; flow cytometry (1:50)
ref: J Immunology 159:206-213 (1997)
-For In Vitro Research Use Only-
R2=IFN-gR2
**on myeloid cell lines (U937, HL60 and THP-1) maximum signal (80-90%) positively is acheived without permeabilizing cells. On umpermabilized B cell lines (EBV cell lines, Raji, Namalwa) R2 positivity decreases (30-50%) whereas on T cell lines (Jurkat, Molt4, ST4, PF382) R2 positivity is about 5-15%
-permebilization helps detection of R2 on T cell lines (cytoplamsic concentration). Permebilized T, B and myeloid cells all show 80-100% R2 positivity.
-by confocal microscopy, visualized R2 in human T cells is :
a) prevalently localized on the cytoplasm of human T cells
b) its accumulation into clathrin coated pits together CTL4 and transferrin receptor
Beta chain (non neutralizing)
CAT# RDI-PB31585 $375.00/vial
Quantity Shipped: 1 vial of 50 ug in 0.1 ul PBS with 0.1% BSA
Concentration: 0.5mg/ml.
Bioactivity: Binds to but does not neutralize human interferon gamma receptor.
Usage: Western blot; immunoprecipitation; immunohistochemistry; flow cytometry (1:1000 dilution)
Shipping Temperature: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
-For In Vitro Research Use Only-
cat# RDI-MCD119-GRBT 100ug Biotin labeled $344.00
Package Size: 100ug purified in 0.2ml (0.5mg/ml) buffer and 0.09% sodium
azide. (sodium azide is toxic, dilute azide compounds with copious amounts
of water when disposing in drain to avoid accumulation of explosive
deposits-avoid contact with acids.)
Species: rat IgG2a (brown Norway)
CLONE: GR20
Immunogen: IFNG receptor affinity enriched from membranes of the
monomyelocytic cell line WEHI-3
Activity: Reacts with mouse CD119, the alpha subunit of the interferon gamma
receptor. This receptor is expressed by mouse leukocytes, endothelial and
epithelial cells but not mature erythrocytes. Clone GR20 Mab recognizes an
epitope susceptible to certain fixatives in the ligand-binding site of the
receptor, therefore it block binding of mouse IFN-G. It has been reported
that GR20 antibody can prevent the ability of mouse IFNG to prime macrophages
for tumor cell killing. IFNG has been reported to induce the expression of
LY-6A/E antigen on cell lines and normal B cells. It has been observed that
GR20 Mab inhibits IFN-G induced expression of Ly-6A/E on the mouse B lymphoma
line A20. Binding of the GR20 ab to CD119 is inhibited in the presence of
IFN-G since the GR20 antibody recognizes an epitpe in the ligand binding
site of the IFNGR. This antibody reportedly does not have any agonist
effect.
Application: -Indirect immunofluorescence cell surface staining (flow cytometry)
-immunoprecipitation (purified /non conjugated ab)
-western blot (non reducing conditions)
-immunocytochemistry
-ELISA
-purification of detergent solubilized receptor
References: 1) Proc Natl Acad Sci USA 85:6282-6286
2) J. Leukoc Biol 51:507-516
3) J Biol Chem 265:4064-4071
4)
J. Immunol 144:2197-2204
Storage: Store at 4 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
(CD119) MONOCLONAL ANTIBODY (Nonblocking Antibody)
cat# RDI-MCD119-2E2 $500.00
Presentation: 0.5mg in 1mg (0,5mg/ml) buffer with 0.09% sodium azide
Clone: 2E2
Immunogen: purified preparation of soluble recombinant mouse IFN-GRalpha chain protein.
Isotype: Armenian Hamster IgG
Purification: purified from tissue culture supernatant via Protein A affinity chromatography
Storage: The antibody solution should be stored undiluted at 4°C.
Use: -FACS (1ug/million cells):clone 2E2 MAB is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the ligand (i.e., IFN-G ) for IFN-G receptors is present. A three-layer staining protocol is recommended for maximizing the detection IFN-GRa chains expressed by cells .
-IP: The 2E2 antibody has been reported to be useful for the immunoprecipitation of IFN-GRa chains from lysates of cloned mouse T cells.
Background: The 2E2 antibody recognizes the extracellular region of the 90 kDa alpha chain subunit of the mouse interferon- receptor (IFN- R ; aka, CD119). The functionally active-form of the mouse IFN- receptor consists of two (or more) subunits, with IFN- R responsible for IFN- binding and both the IFN- R and IFN- R chains required for the transduction of biologic responses. IFN- R is expressed by a variety of cell lines and normal mouse cells (except mature erythrocytes) including T cells, B cells, NK cells, monocytes, neutrophils, fibroblasts, epithelial and endothelial cells. The 2E2 antibody is a non-neutralizing antibody; it does not block the binding of IFN-G to its receptor.
For In Vitro Research Use Only-not responsible for patent infringement or other violations that may occur with the use of our products.
REFERENCES:
1. Bach, E. A., S. J. Szabo, A. S. Dighe, A. Ashkenazi, M. Aguet, K. M. Murphy, and R. D. Schreiber. 1995. Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets. Science 270:1215-1218.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
see also hamster anti-mouse IFNGRB clone MOB-47 cat#RDI-MIFNGRB-47 $500.00/0.5mg
Sample staining protocol:
BALB/c spleen cells (including erythrocytes) were preincubated (~15 min., 4°C) with purified 2.4G2 antibody [rat anti-mouse CD16 /CD32 (Fc II); 1 µg antibody/10 6 cells]. The cells were stained (30 min., 4°C) with purified 2E2 antibody (0.5 µg mAb/10 6 cells; After washing, the cells were incubated (30 min., 4°C) with a biotin-conjugated cocktail of mouse anti-hamster antibodies (Clones G70-204 + G94-56; Cat. No. RDI-12102D $375.00; 0.5 µg mAb cocktail/10 6 cells). Finally, the cells were washed and incubated with R-PE-conjugated streptavidin 0.015 µg PE-SA/10 6 cells) and FITC-anti-CD4 (clone RM4-5;) and FITC-anti- CD8 (clone 53-6.7; ), both at 0.06 µg mAb/10 6 cells. After washing, the cells were analyzed with a FACScanZ Flow Cytometer. The data indicates that mouse erythrocytes are uniformly negative for IFN-GR alpha expression whereas most T cells (i.e., CD4 + /CD8 + cells) and CD4-CD8-. lymphocytes constitutively express medium levels of IFN-GR alpah chains.
cat# RDI-MIFNGRB-47 $500.00
Presentation: 0.5mg in 1ml (0.5mg/ml) buffer with 0.09% sodium azide
Clone: MOB-47
Isotype: Armenian Hamster IgG
Immunogen: purified soluble extracellular domain of the mouse IFN-GR beta chain protein.
Purification: purified from tissue culture supernatant via Protein A affinity chromatography.
Storage: The antibody solution should be stored undiluted at 4°C.
Use: -FACS:1ug/million cells (1 µg antibody/10 6 cells) A three-layer staining protocol is recommended for maximizing the detection IFN- R chains expressed by cells
-IP/WB: The MOB-47 antibody has been reported to be useful for the immunoprecipitation and Western blotting of IFN-GR beta chains from lysates of cloned mouse T cells.
background: The MOB-47 antibody recognizes the 60-65 kD beta chain subunit of the mouse interferon- receptor (IFN- R ). The IFN- receptor beta chain is expressed by a variety of normal mouse cell types and cell lines that are responsive to IFN- including T and B lymphocytes, NK cells, monocytes, macrophages, granulocytes and fibroblasts. The IFN- R receptor epitope recognized by the MOB-47 antibody is blocked by bound IFN-G .
Note: The IFN-GRbeta receptor epitope recognized by the MOB-47 antibody is blocked by bound IFN-G . Thus, it has been reported that cells which have bound IFN- 1 can be pretreated with dilute acid to remove the bound ligand before they are stained.
REFERENCES:
1. Bach, E. A., S. J. Szabo, A. S. Dighe, A. Ashkenazi, M. Aguet, K. M. Murphy, and R. D. Schreiber. 1995. Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets. Science 270:1215-1218.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For Research Use only
sample staining protocol:
BALB/c spleen cells and A20 cells (mouse B cell lymphoma) were preincubated (~15 min., 4°C) with purified 2.4G2 antibody [rat anti-mouse CD16 1 µg antibody/10 6 cells] to block nonspecific staining due to immunoglobulin Fc receptors. The cells were stained (45 min.,4°C) with purified MOB-47 antibody (either at 0.5 µg mAb/10 6 BALB/c cells or at 0.125 µg mAb/10 6 A20 cells; . After washing, the cells were incubated (30 min., 4°C) with a biotin-conjugated cocktail of mouse anti-hamster antibodies (Clones G70-204 + G94-56; Cat. No.RDI-12102D; 0.5 µg $375.00 mAb cocktail/10 6 cells). Finally, the cells were washed and incubated with R-PE-conjugated streptavidin (0.015 µg PE-SA/10 6 cells). After washing, the cells were analyzed with a FACScanZ Flow Cytometer.
RECEPTOR CHAIN 1
CAT# RDI-PB32580 $375.00/vial
Description: Rabbit Anti-Mouse Interferon Gamma Receptor Chain 1,
non-neutralizing.
Quantity Shipped: 1 vial of 50 ug in 50 ul.
Concentration: 1 mg/ml.
Bioactivity: Binds to but does not neutralize mouse interferon gamma receptor.
Usage: Western blot; immunoprecipitation; immunohistochemistry; flow cytometry.
Shipping Temperature: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
-For In Vitro Research Use Only-
RABBIT POLYCLONAL ANTIBODY AGAINST MOUSE INTERFERON GAMMA RECEPTOR
CHAIN 2 (non neutralizing)
CAT# RDI-PB32585 $375.00/vial $344.00/vial 3+
Quantity Shipped: 1 vial of 50 ug in 50 ul.
Concentration: 1 mg/ml.
Bioactivity: Binds to but does not neutralize mouse interferon gamma receptor.
Usage: Western blot; immunoprecipitation; immunohistochemistry; flow cytometry
Shipping Temperature: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
-For In Vitro Research Use Only-
Catalog#: RDI-IFNGRabm-B $625.00/vial
Package Size: 250ug purified IgG (by Protein G) in 0.25ml (1mg/ml)
PBS buffer. NO preservative or carrier protein added. Sterile filtered.
Species: mouse IgG1k
Clone: GIR-208
Antigen: purified human interferon gamma receptors obtained from human placenta.
The antigen appeared as a single predominant band at 80-90Kd on silver stained
SDS-Page.
Specificity: binds specifically to human Interferon gamma receptor. The binding
is inhibited by excess IFN-G. No cross with IFN-G was detected in Elisa.
Activity: -Immunocytochemistry: Frozen sections of human placenta and human
renal biopsy (J. Immunol 149:2675(1992), Transplantation Proc., 25:917 (1993)
-Western blot: under non reducing conditions (J. Immunol, 140:4231 (1988)
-Immunofluorescence:2-5ug
-Neutralization: (J Immunol 140:4231-4237) 1988, Cell Immunol 138:265-279, Cell Immunol 149:2675-2680 (1991)
Storage: Store 4-8 DEG C under aseptic conditions.
Precautions: For In vitro research Use Only.
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.
also available:
PE conjugated cat#RDI-IFNGRabm-BPE $594.00/0.2mg
Catalog#: RDI-IFNGRabm4 $500.00/vial
LOT# see batch sheet
Package Size: 500 ug purified IgG in 1ml PBS (0.5mg/ml) PBS with 0.09% sodium
azide.
Species: mouse IgG2bk
Clone: GIR-94
Antigen: purified human interferon gamma receptors. The antigen appeared
as a single predominant band at 80-90Kd on SDS-Page.
Specificity: binds specifically to human Interferon gamma receptor. The binding
is inhibited by excess IFN-G. No cross with IFN-G was detected in Elisa.
Activity: -Western blot: approx 1ug/ml of a biotinylated ab will detect approx
0.5ug of purified IFNG-R under non reducing conditions
-Immunofluorescence:fluorescence of IFNG-R bearing cells is not inhibited
by prior incubation with excess (1.5ug) IFN-G. Use approx 2ug antibody to
stain IFNG-R present on 0.3-2 X 106 COLO-205 cells.
Storage: Store 4-8 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this products.
PE conjugated cat#RDI-IFNGRabm4-PE $594.00/0.2mg
HUMAN INTERFERON GAMMA SOLUBLE RECEPTOR
cat#RDI-PB11580 $438.00
Description: Human Interferon Gamma Receptor Chain 1 (a chain)
Lot#:
Quantity Shipped: 10 ug
Concentration: 0.1 mg/ml
Buffer: 6M urea, 20mM tris, 125 mM NaC1
Molecular Weight: tbd
Purity: >95%
Source: mRNA (cDNA) obtained from human cells expressed in baculovirus vector
Bioactivity: binds human interferon gamma
Shipping Information:
Shipping temperature: ice Pack
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-70'C or below for retention of full activity. When thawing, the contents
of the tube should be apportioned in separate tubes so that freezing and
thawing is kept to a minimum. Dilute only in buffers containing protein such
as 0.1% bovine serum albumin (BSA).
This Product Is For Research Use Only. It Is Not For Human Use.
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (800) 370-2222 or
(978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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