rev:   October 1, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Granulocyte colony-stimulating factor (G-CSF) Receptor ANTISERA

 see also CD114 antibodies

Mouse Anti-G-CSFR receptor (HUMAN reactive) CD114

Catalog#: RDI-CD114-LM $562.00/vial

also available:Biotin labeled cat#RDI-CD114-LMBT $625.00/0.5mg

Package Size: 500ug purified in 1ml (0.5mg/ml) 10mM sodium phosphate pH7.2 with 150mM NaCl and 0.09% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits- avoid contact with acids.)

Prep: purified by Protein G from TCS

Species: mouse IgG1k

CLONE: LMM741 Workshop Code:VI, MA98

Activity: Reacts with the human granulocyte-colony stimulating factor(G-CSF) receptor. G-CSFR are expressed on the neutrophils, myeloid leukemias, endothelium, platelets and placenta. The immunogen for this antibody was the CH0-6A11 cell line which was generated by transfection of CHO cells with an expression vector containing a full length cDNA encoding the human G-CSF receptor. Binding approx position 332-346

Application: -Indirect immunofluorescence cell surface staining (flow cytometry) , Titer for each application -recommend approx 2ug/million cells, use Streptavidin:PE, positive staining on human peripheral blood granulocytes and TF-1 and U-937 cell lines but not with human peripheral blood lymphocytes and Raji and JY cell lines.

-western blot (on peripheral blood granulocytes=2 bands 130kDa and a minor band at 95kDa).

-immunoprecipitation

-blocking(azide free)

References:

1) Leucocyte Typing VI, Garland Press, NY 1997

2) Proc Natl Acad Sci USA 91:2985-2988 (1994)

Storage: Store at 4 DEG C.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

For in vitro research use only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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