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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Anti-EGFR MONOCLONAL  Antibodies
see also anti-EGFR polyclonals

PRODUCT CODE     RDI-CBL416 $375.00/vial

Also available: FITC conjugated cat#RDI-CBL416FT $375.00/100T

Biotin conjugated cat#RDI-CBL416BT  $375.00/1000T

CLONE         EGFR1

ISOTYPE     IgG2b

SOURCE       Tissue culture supernatant

PURIFICATION METHOD    Protein A affinity chromatography

SPECIFICITY This antibody reacts with the 175 kDa molecular weight cell surface marker from the epidermoid carcinoma cell line A431. EGFR1 is directed against an antigenic site on the extracellular domain of human EGF receptor. Various cell lines known to express EGF receptor are immunostained including KB and A431.

APPLICATIONS * Indirect immunofluorescence on fixed cell suspension* Isolation of human EGF receptor* Flow cytometry

REFERENCES Waterfield, M. D. et al. J. Cell. Biochem. 20, 149-161 (1982)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available in bulk, cat#RDI-CBL416-1XP (No Bsa/No Azide)   $1125.00/1mg  Larger sizes quoted on request

PRODUCT CODE     RDI-CBL417    $375.00/vial

CLONE            F4

ISOTYPE         IgG1

SOURCE          Mouse ascitic fluid

PURIFICATION METHOD Protein A affinity chromatography

SPECIFICITY This antibody is specific for the intracellular domain of the human epidermal growth factor receptor.

APPLICATIONS *Reactive with fixed tissue showing strong squamous cell carcinoma staining*Antibody immunoprecipitates EGF receptor*Relevant in studies of growth factor receptors to loss of growth control in tumours

REFERENCES Gullick et al. Canc. Res. 46, 285-292 (1986)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Bulk on request

Catalog#: RDI-EGFRACTabm

Price: $500.00/50ug    $938.00/150ug

-BULK QUOTES ON REQUEST-

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA

Species: mouse IgG1

Activity: The antibody reacts only with the tyrosine phosphorylated (activated) EGF receptor.

Antigen: tyrosine-phosphorylated epidermal growth factor receptor isolated from EGF-challenged mouse L-cells expressing the human EGF receptor.

Use: western blot (1:1000), band approx 180Kda with incubation 2-3 HR RT or overnight at 4 DEG C with chemilimunescence detection) or indirect immunofluorescence on human (not reactive with mouse or other species). **

ref: Carraway, C.A. et al , J. Biol. Chem (1993) 268:5582

      -Leaman, D.W. et al, 1996 Mol Cell Biol 16:369-375

Storage: -20 DEG C long term. 4 DEG C short term (AVOID FREQUENT FREEZE THAW CYCLES.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

- NOT RESPONSIBLE FOR ANY PATENT INFRINGEMENTS THROUGH USE OR DERIVATION OF THESE PRODUCTS.

**a companion antibody is available which reacts with both stimulated and unstimulated and reacts with human and mouse by western blot 1:2500 dilution. mIgG1.  order cat#RDI-EGFRHMabm $375.00/50ug

Catalog#: order cat#RDI-EGFRHMabm

Price: $438.00/50ug (0.2ml) $750.00/150ug (0.6ml)   -BULK QUOTES ON REQUEST-

Package Size: purified IgG1 at 0.25mg/ml in 50% glycerol, 20mM sodium phosphate pH 7.5, 150mM NaCl, 1.5mM NaN3, 1mg/ml BSA

Species: mouse

Activity: The antibody reacts only with EGF receptor on human and mouse by western blot MW 180kD.

Antigen: synthetic peptide coupled to BSA corresponding to residues 996-1022 of the human EGFR.

Use: western blot (1:2500), indirect immunofluorescence , immunoprecipitation (under native or denatured conditions, 2-4ug)

ref: -Carraway, C.A. et al , J. Biol. Chem (1993) 268:5582

      -Campos-Gonzalez, R. and Glenney J.R. Jr (1991) Cell Reg. 2:663.

Storage: -20 DEG C long term. 4 DEG C short term (AVOID FREQUENT FREEZE THAW CYCLES.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or   for diagnostics.

DATA SHEET: Mouse anti-Non Phosphorylated EGF-R HUMAN/Mouse

Catalog#: RDI-EGFRNPabm    Price: $562.00/vial

Package Size: 50 micrograms mIgG1 in Tris/glycine/glycerol

Antigen: synthetic peptide from human EGF-R (NAE{Y1173}LR...

Species: mIgG1

Activity: Reacts with the unphosphorylated EGFR, does not recognize phosphorylated pY1173 EGF receptor. Recognizes EGFR at 175kDa.

Species: human,mouse

Uses: -western blot: 0.5-2microgram/ml detects 175kDa EGFR in RIPA extracts of HFF cells (negative control EGF stimulated HF cells).

          -immunoprecipitation: 1ug

ref: J. Biol Chem 267:8672, 1992

Storage: -20 DEG C long term. 4 DEG C short term AVOID FREQUENT FREEZE THAW CYCLES.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

- NOT RESPONSIBLE FOR ANY PATENT INFRINGEMENTS THROUGH USE OR DERIVATION OF THESE PRODUCTS.

Catalog#: RDI-EGFRPabm-H2     Price: $562.00/vial

Package Size: 50 micrograms mIgG1 in Tris/glycine/glycerol

Antigen: synthetic phosphopeptide NAE(py)LRV representing the major autophosphorylation site of EGFR (NAE{Y1173}LR...

Species: mIgG1

Clone: 9H2

Activity: Reacts with the phosphorylated EGF-R, Py1173. Recognizes EGFR at 175kDa.

Species: human,mouse

Uses: -western blot: 0.5-2microgram/ml detects 175kDa EGFR in EGF stimulated RIPA extracts of HFF cells).

          -immunoprecipitation: 1ug

ref: -J. Biol Chem 267:8672, 1992

      -J. Biol. Chem263:3610, 1988

Storage: -20 DEG C long term. 4 DEG C short term AVOID FREQUENT FREEZE THAW CYCLES.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

- NOT RESPONSIBLE FOR ANY PATENT INFRINGEMENTS THROUGH USE OR DERIVATION OF THESE PRODUCTS.

Antigen: partially purified human EGFR

Purification: Protein A

Species: mIgG1

Clone: LA1

Activity: Reacts with extracellualr domain of human EGFR

Uses: -western blot: 1microgram/ml detects 180kDa EGFR in A431 cell lysate.

          -immunoprecipitation: 4ug

           -neutralization: 0.5-10ug/ml

           -cytochemsitry


ref: -J. Biol Chem 268:2924:1993

       -Nature 377:158, 1995

       -J. Biol Chem 272:5792, 1997

       -Neuron 19:251, 1997

Storage: -20 DEG C long term. 4 DEG C short term AVOID FREQUENT FREEZE THAW CYCLES.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  - NOT RESPONSIBLE FOR ANY PATENT INFRINGEMENTS THROUGH USE OR DERIVATION OF THESE PRODUCTS.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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