rev:   March 3, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


SCF-R (STEM CELL FACTOR Receptor C-Kit) ANTISERA CD117
CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CD117-A5               57A5                          ---          mIgG1      purified       $312.00

RDI-CD117-A5BT          57A5                          ---          mIgG1     Biotin          $344.00

RDI-CD117-A5PE           7A5                          ---          mIgG1       PE              $500.00

RDI-CD117abRM           SP10                          --         rabbit monoclonal             $438.00


mouse anti-human CD117 clone 57A5  

cat#RDI-CD117-A5        $312.00/vial 100ug  $281.00/vial 3+

cat#RDI-CD117-A5BT    $344.00/vial 100ug Biotin conjugated

       RDI-CD117-A5PE    $500.00/120T PE conjugated

-clone 57A5 recognizes epitope 2 of the CD117 molecule of about 150kd.

-mIgG1

-sutiable for flow cytometry, immunoprecipitation and frozen sections

-does not block binding of SCF

ref: Leukocyte Typing VI, Garland Publishing Inc New York (1997) p 816-818


Rabbit anti-CD117(C-kit/SCF-Receptor) Monoclonal

cat# RDI-CD117abRM    $438.00/0.5ml

       RDI-CD117abRM1   $750.00/1ml

Presentation: tissue culture supernatant with 15mM sodium azide

Clone ref: SP10

Isotype: rabbit IgG

Mol. Wt. Antigen: 145kDa

Immunogen: A synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein.

Epitope: Cytoplasmic

Species Reactivity: Human, and Dog. Others not known.

Applications: -Immunohistology (Formalin/paraffin only) (Use Ab 1:40-1:100 with enhnaced detection (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH6.0 for 10-20 min followed by cooling at RT for 20 min.) The optimal dilution for a specific application should be determined by the investigator

Positive Control: Skin, GI Stromal Tumor(GIST).

Cellular Localization: Cell membrane / cytoplasmic

Storage: at 2-8°C.

Description: This rabbit moncoclonal antibody does not interfere with the binding of SCF to c-kit. It precipitates both the unoccupied as well as the occupied form of c-kit. The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration, or survival. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis.

For Research Use Only


Rabbit anti-CD117(C-kit/SCF-Receptor) polyclonal

cat# RDI-CD117abrX $438.00/0.5mg

        RDI-CD117abrX1 $688.00/1mg

       RDI-CD117abrXP $750.00/1mg no BSA/NO AZIDE

       RDI-CD117abrX-CP $188.00 (blocking peptide) 200ug

Presentation: Total IgG purified from rabbit anti-serum by Protein A chromatography. Prepared at 4.0mg/ml in 10mM PBS, pH 7.4, with 0.2% BSA & 0.09% sodium azide. Also available without BSA and azide at 4mg/ml.

Mol. Wt. Antigen: 145kDa

Immunogen: A synthetic peptide from C-terminus of human CD117 / c-kit protein.

Epitope: C-terminal

Species Reactivity: Human, and Dog. Others not known.

Applications: -Immunohistology (Formalin/paraffin only) (Use Ab (5-10ug/ml for 30-60 min at RT) (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 or 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.) The optimal dilution for a specific application should be determined by the investigator

Positive Control: Skin, GI Stromal Tumor(GIST).

CellularLocalization: Cell membrane / cytoplasmic

Storage: at 2-8°C.

Description: c-kit is a transmembrane receptor tyrosine kinase. It binds to stem cell factor (SCF). It is expressed in many tissues and cells. C-kit/SCf is involved in development of several lineages of stem cells, such as germ cells, neural crest-derived melanocytes and hematopoietic precursor cells. Antibody to CD117 / c-kit is very helpful in the histopathological identification of gastrointestinal stromal tumors (GIST).


Rat anti-mouse C-KIT (CD117)

cat#RDI-MCD117abrt    $500.00/0.5mg

-rat IgG2a purified, clone 2B8

-suitable for flow cytometry approx1ug/million cells

Rat anti-mouse C-KIT (CD117)

cat#RDI-MCD117-ACK $500.00/0.5mg

-rat IgG2b purified, clone ACK45 -reported to block activity of c-kit

-suitable for flow cytometry and histochemistry


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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