rev:February 5, 1997
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Subclass Book Table of Contents)
The following book on the IgG Subclass is made available by the permisssion
of CLB Reagents (Netherlands). It is for your personal use and may not be
reproduced without the permission of CLB Reagents. This material is for
information use only and should not be construed as medical advice. We are
not responsible for any misprints or error or ommissions. Certain charts
,graphs and table have been ommitted or changed in style (not content)
to fit this web site. Please use this information as part of your discussion
with your medical professional. To order a copy of the 52 page book, please
orrder catalog#RDI-IGGBOOK $12.00 (includes postage and shipping by
mail worldwide). Allow 6-12 weeks for delivery. Offer limited to supply.
6.2 CLB Quality Survey Service for IgG subclass assays
As a service to its customers, the CLB Department of Reagents provides a free Quality Survey for in-house assessment of local human IgG subclass assays. Laboratories in any part of the world may participate.
Twice a year, 3 encoded samples (normal,as well as patient sera) are distributed
among the participating laboratories. Levels of IgG1,IgG2,IgG3,IgG4 and total
IgG are determined by the respective laboratories and all results are evaluated
at CLB. This procedure is suitable to detect any systematic errors in the
assays performed by the participating laboratories.
In figure 11 (inquire for photocopy) we show an example of some
representative data from one of our 1995 Quality Surveys. The participating
laboratories quantified the four IgG subclasses and total IgG in the three
samples, by means of RIA, ELISA,nephelometry, turbidimetry, affinity
chromatography or other techniques with kits or reagents supplied by the
CLB or by other manufacturers. The example given in figure 11 shows the results
of IgG1 determination on three different samples, which were obtained by
one of the laboratories (designated here as 'TEST'),using the CLB-BNA (Behring
nephelometer) kit. These results are compared with those from other participating
laboratories using the same technique and reagents, as well as with the overall
results, obtained by all techniques used. The data are presented in the format
of histograms. By regularly participating in such Quality Surveys, laboratories
will be able to check the reliability of their tests.
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