rev:February 3, 1997

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Trypsin Pre-Treatment Protocol

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

TRYPSIN PRE-TREATMENT

1. Stock Solutions

a) Trypsin   Trypsin (type II-porcine pancrease)          4g
                   Distilled Water                                  100ml

Aliquot in 5ml amounts. Freeze until required.

b) Calcium Chloride

          Calcium Chloride 4g

          Distilled Water 100ml

Aliquot in 5ml amounts. Freeze until required.

2. Working Solution

a) Thaw one 5ml aliquot of both trypsin and calcium chloride.

b) Dissolve in 190ml distilled water.

c) pH to 7.8 with IN NaOH.

d) Warm to 37'C for use.

3. Protocol

a) Incubate slides for 10-30 minutes* at 37'C.

b) Wash in serveral changes of buffer and then perform selected procedure.

*Note: Time of incubation may need to be varied depending on tissue, and type of degree of fixation.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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