rev: 5/16/2008
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of CD monoclonal antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD74
Mouse anti-human CD74 antibodies
-2 clones
CATALOG# CLONE# Workshop Host Form Price
RDI-CBL532 LN2 IV mIgG1 purified $375.00 also FITC & Biotin
RDI-CD74abm-41 M-B741 mIgG2a puridied (also availabeleBT, FT and PE) $346.50
MOUSE MONOCLONAL ANTIBODY TO: HUMAN CD74
PRODUCT CODE RDI-CBL532 $375.00/vial
MOUSE MONOCLONAL
CLONE LN2
ISOTYPE IgG1, kappa
IMMUNOGEN Nuclear homogenates from the diffuse histiocytic lymphoma cell line SU-DHL-4
SOURCE Tissue culture supernatant
PURIFICATION
METHOD Protein A affinity chromatography
SPECIFICITY This antibody recognizes type II integral membrane glycoproteins (35 kDa) present on the nuclear membrane of B cells. This antigen is mainly expressed on the cell surface of resting and activated B cells. In immunoprecipitation studies, LN-2precipitates three molecules from S-labelled RJ2.2.5 cells: a weak 41-kDa band, a 35-kDa band and a strong 33 kDa band. Ln-2 has been demonstrated to react with an epitope residing within 60 amino acids of the extracytoplasmic C-terminus of the molecule.The invariant chain, CD74 is involved in MHC class ll-restricted antigen presentation and processing.
APPLICATIONS * Studies of B cells from follicular centre and mantle zone. Also labelled using Heat Induced Epitope Retrieval (HIER)* Studies on formalin fixed paraffin embedded sections*Distinguishing B cell lymphomas from T cell lymphomas. B-cell leukaemias corresponding to different stages of B-cell maturation react positively with LN-2 including pre-B and B-lineage common acute lymphoblastic leukemia (ALL), chronic lymphocytic leukemia (B-CLL) and hairy cell leukaemia. By immunohistochemistry, LN-2 has been shown to label B-cell non-Hodgkin's lymphoma on routinely processed tissue. Small lymphocytic and small cleaved lymphomas have been observed to react most strongly; however large cell lymphomas are also labelled with LN-2. Mononuclear Hodgkin cells and Reed-Sternberg cells in Hodgkin's disease have been demonstrated to stain positively, as do some rare T-cell lymphomas. Among non-lymphoid neoplasms, LN-2 has been observed to label some epithelial tumours including adenocarcinoma of the uterus, squamous cell carcinoma of the lung and transitional cell carcinoma of the bladder.Langerhans cells and cells of histiocytosis or histiocytosis X have demonstrated positively with LN-2.
REFERENCE Epstein, A. L. et al. J. Immunol 133, 1028 - 1036 (1984)Marder, R. J. et al. Lab. Invest 52, 497-504 (1985)Leucocyte Typing IV, Oxford University Press (1989)Leucocyte Typing V, Oxford University Press (1995)Okon, E. et al. Cancer 56-95 (1985)Azumi, N. et al. Human Pathol 19(12):1376 (1988)Brownell M et al. J. Surg Pathol 11(10):779 (1987)
PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
Also available:
FITC conjugated cat#RDI-CBL532 $375.00/100T vial
Biotin conjugated cat#RDI-CBL532BT $375.00/vial 100T
Bulk on request
Bulk on requestFor Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
Mouse anti-human CD74 clone M-B741
cat#RDI-CD74abm-41 $312.00/100ug purified
cat#RDI-CD74abm-41PE $500.00/120T PE conjugated
cat#RDI-CD74abm-41FT $375.00/120T FITC conjugated
cat#RDI-CD74abm-41BT $375.00/100ug Biotin conj
Bulk quotes on request
Package Size: 100ug in 0.1ml (1mg/ml) 50mM sodium phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5mg/ml gentamicin sulfate.
Prep: purified by Protein A from TCS. Purity >95% by SDS-Page.
CLONE: M-B741
Isotype: Mouse IgG2a
Immunogen: RPMI 1788 Cell Line
INFORMATION: Human CD74 is expressed intracellularly and on the cell surface of
MHC Class II positive cells such as B cells, monocytes, macrophages, Langerhans
cells and dendritic cells. CD74 has several important functions relating to
antigen presentation by MHC Class II molecules. Antibody M-B741 recognizes the
CD74 molecule of about 33 kd.
References: Leukocyte Typing V (S.F. Schlossman, et al, eds.)
STORAGE CONDITIONS: Store at 2 - 5oC . Freeze/Thawing is not recommended.
PERFORMANCE: Briefly, 500K cultured Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD74 antibody at 5 m g/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC at a 1:60 dilution factor, after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.09 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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