rev: October 6, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD72  Antibodies

Mouse anti-human CD72  antibodies

-1clone 

CATALOG#                 CLONE#                  Wokshop    Host         Form        Price

RDI-CBL460                   BU40                            IV          mIgG2a     purified    $375.00

  see also anti-mouse CD72 monoclonal and polyclonals below

Mouse ANTI-Human CD72 MONOCLONAL ANTIBODY

PRODUCT CODE :RDI-CBL460     $375.00/vial 200ug

CLONE: BU40

ISOTYPE: IgG2a

SOURCE: Mouse ascitic fluid.

PURIFICATION METHOD: Ion exchange chromatography following ammonium sulphate precipitation.

SPECIFICITY: A heterodimeric glycoprotein of 43/39kD. Antigen is expressed on all stages of B-cell differentiation from early to late.

Antigen distribution: B-cells(Ig+,E-) 96 ± 3%

Peripheral blood lymphocytes 13 ± 4%

T-cells (E+) < 1

NK-cells < 1%

Thymocytes < 1%

Granulocytes < 1%

Monocytes < 1%

APPLICATIONS:

* Leukaemia and lymphoma phenotyping

* Studies of CD72 antigen expression which is increased upon B cell stimulation.

REFERENCES: Leucocyte Typing IV, pp27, Oxford University Press (1989).

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200µg/2ml in phosphate buffered saline solution containing 10mM sodium azide & 1mg/ml BSA. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in bulk (NO BSA/no AZIDE) $1000.00/milligram

Larger quantities quoted upon request.

                                                      $438.00/3 or more BULK ON REQUEST

Package Size: 0.5mg in 1.0ml 50mM Tris-HCL pH 8.0 with 150mM NaCl and 0.1% sodium

azide.

Supplied: purified by affinity chromatography

Clone: 10-1.D2

Ig Isotype: mouse (type C57BL/6) IgG2bk

Reactivity: ** -Reacts with the CD72.1, the ligand of CD5 found on B lymphocytes in strains expressing the Lyb-2.1 allotype (e.g. CBA/J, C3H/Bi, C57L, C58, DBA/1, DBA/2, SWR, BUT NOT A, AKR, Balb/c, CBA/CA, CBA/N, C3H/He, C57BL, NZB, SJL, 129).

-Clone 10-1.D.2 blocks the binding of C5 to CD72 on the B cell surface and it enhances in vitro B cell responses.

Uses: flow cytometry (<1ug/1M cells), function blocking studies (must remove azide by dialysis or equivalent and possibly remove any endogenous endotoxin before use as blocking agent).

ref:
J. Immunol. 148:1630-1634

J. Immunol. 137:1793-1796

J. Immunol. 130:2033-2037

J. Immunol. 149:880-886

Storage: Store at 4 Deg C.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

also available: Fitc labeled: cat#RDI-mCD72abm-FT      $594.00/0.5m

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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