rev: February 10, 2002

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD71  Antibodies


Mouse anti-human CD71  antibodies


CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CBL137                  RVS10                           --           mIgG1      purified     $375.00

RDI-CBL137FT                   "                               --              "            FITC        $375.00

RDI-CBL137PE                   "                               --              "            PE            $470.00


Mouse ANTI-Human CD71 ANTIGEN

PRODUCT CODE :RDI-CBL137     $375.00/200ug vial

also available: FITC labeled cat#RDI-CBL137FT    $375.00/100 Tests

                          PE labeled     #RDI-CBL137PE    $469.00/100 Tests


CLONE: RVS-10

ISOTYPE: IgG1

SOURCE: Tissue culture supernatant

PURIFICATION METHOD: Ammonium Sulphate precipitation followed by ion exchange chromatography

SPECIFICITY: Human Transferrin Receptor which is expressed on proliferating lymphoblasts, majority human tumour cell lines, normal peripheral blood monocytes

Antigen distribution: Con A activated lymphocytes 67 ± 27%

PHA activated lymphocytes 67 ± 27%

Monocytes 40 ± 20%

Thymocytes 22 ± 7%

Peripheral blood lymphocytes < 3%

B-cells (E-) < 1%

T-cells (E+) < 1%

NK-cells (CD16+) < 1%

Granulocytes < 1%


APPLICATIONS:

* Study of cellular proliferation and lymphocyte activation

* Cell surface expression of transferrin receptors is correlated with relative cell growth rate of malignant and non-malignant cells

* Transferrin receptors are virtually absent from resting PBL's


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Bulk purified available upon request without BSA and preservative (approx $1125.00/milligram) ord cat#RDI-CBL137-1XP


cat#RDI-TRK4TR26-      $438.00/1mg    $375.00/mg 2-5mg     $312.00/mg 6-20mg

Monoclonal anti-human Transferrin Receptor


Clone: 7-1 (cat#RDI-TRK4TR26-71)      

           2B6 (cat#RDI-TRK4TR26-2B6)

           5B11, 7F8, 10D2, 11F5, 13E4, 23D10  cat#RDI-TRK4TR26-####   ###=clone#)

 $438.00/mg   $375.00/mg 2-9mg, $312.00/mg  10-20mg

These clones have been derived from hybridization of   Sp2/0 myeloma cells with spleen cells of a Balb/c mice   immunized with purified transferrin receptor

Isotype: IgG2b for clone 7-1, 5B11, 10D2, 1F5, 23D10,

              IgG2a for clones 2B6, 7F8, 13E4

Purification: Protein A Sepharose Purity tested by electrophoresis

Specificity: human soluble transferrin receptor

Reactivity: Detection of human soluble transferrin receptor.

                Antibodies are working in ELISA.

              Clones 2B6, 5B11, 7F8, 10D2, 1F5, 13E4, 23D10 are working in Western blotting.

                (clone 1F5 seems best for WB)

Best recommended pairs for ELISA :

coating - detection

2B6 - 1F5

7F8 - 23D10

5B11 - 2B6

10D2 - 13E4

1F5 - 7F8

23D10 - 13E4

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 1 milligram of murine monoclonal antibody dissolved in 0.480ml (2.1mg/ml) of phosphate buffered saline containing 0.1% sodium azide. We recommend that each laboratory determine an optimum working titer for use in its particular application.

Storage: Purified monoclonal antibodies are generally stable for period at 4 0C. However repeated warming to room temperature and re-cooling may result in loss of activity and hence working titre.

FOR IN VITRO RESEARCH USE ONLY. NOT FOR USE IN OR ON HUMANS OR ANIMALS-NOT FOR DIAGNOSTICS. Not responsible for any Patent infringements with use or derivation of these products. Contains sodium azide (0.1%)- observe proper precautions.


Human Transferrin Receptor

CAT#: RDI-TRSFRNREC-AG     $625.00/100ug       $938.00/0.5mg    $1250.00/1mg  - Discontinued

Source: Human placenta. Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 ,HIV-2 and HCV antibodies.

Purification: Transferrin receptor was isolated from human placenta by modified method of Anderson G.J., MacKerras A., Powell L.W. and Halliday J.W(Biochim. Biophys. Acta 884, 225-233, 1986)

Presentation: Lyophilized from 50mM HEPES pH 7.4, 1M KCL, 0.1% CHAPS, 0.01% NaN3. It is recommended that the product should be reconstituted in distilled water.

Analysis: Purity more 95% according to SDS-PAGE

Immunological activity confirmed by reaction with monoclonals which are specific to human transferrin receptor.

Storage: At -20'C in lyophilized form. After reconstitution the solution should be aliquoted and stored at- 20'C. Avoid frequent freeze thaw cycles.

For In Vitro Research Use Only-Not for Use in or on human or animals-notfor use in diagnostics

see also other forms available cat#RDI-SCP1858 from human serum/plasma

see main antigen page


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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