rev: February 10, 2002
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Mouse anti-human CD71 antibodies
CATALOG# CLONE# Workshop Host Form Price
RDI-CBL137 RVS10 -- mIgG1 purified $375.00
RDI-CBL137FT " -- " FITC $375.00
RDI-CBL137PE " -- " PE $470.00
PRODUCT CODE :RDI-CBL137 $375.00/200ug vial
also available: FITC labeled cat#RDI-CBL137FT $375.00/100 Tests
PE labeled #RDI-CBL137PE $469.00/100 Tests
SOURCE: Tissue culture supernatant
PURIFICATION METHOD: Ammonium Sulphate precipitation followed by ion exchange chromatography
SPECIFICITY: Human Transferrin Receptor which is expressed on proliferating lymphoblasts, majority human tumour cell lines, normal peripheral blood monocytes
Antigen distribution: Con A activated lymphocytes 67 ± 27%
PHA activated lymphocytes 67 ± 27%
Monocytes 40 ± 20%
Thymocytes 22 ± 7%
Peripheral blood lymphocytes < 3%
B-cells (E-) < 1%
T-cells (E+) < 1%
NK-cells (CD16+) < 1%
Granulocytes < 1%
* Study of cellular proliferation and lymphocyte activation
* Cell surface expression of transferrin receptors is correlated with relative cell growth rate of malignant and non-malignant cells
* Transferrin receptors are virtually absent from resting PBL's
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
Bulk purified available upon request without BSA and preservative (approx $1125.00/milligram) ord cat#RDI-CBL137-1XP
Monoclonal anti-human Transferrin Receptor
Clone: 7-1 (cat#RDI-TRK4TR26-71)
5B11, 7F8, 10D2, 11F5, 13E4, 23D10
$438.00/mg $375.00/mg 2-9mg, $312.00/mg 10-20mg
These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified transferrin receptor
Isotype: IgG2b for clone 7-1, 5B11, 10D2, 1F5, 23D10,
IgG2a for clones 2B6, 7F8,
Purification: Protein A Sepharose Purity tested by electrophoresis
Specificity: human soluble transferrin receptor
Reactivity: Detection of human soluble transferrin receptor.
Antibodies are working in ELISA.
Clones 2B6, 5B11, 7F8, 10D2, 1F5, 13E4, 23D10 are working in Western blotting.
(clone 1F5 seems best for WB)
Best recommended pairs for ELISA :
coating - detection
2B6 - 1F5
7F8 - 23D10
5B11 - 2B6
10D2 - 13E4
1F5 - 7F8
23D10 - 13E4
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 1 milligram of murine monoclonal antibody dissolved in 0.480ml (2.1mg/ml) of phosphate buffered saline containing 0.1% sodium azide. We recommend that each laboratory determine an optimum working titer for use in its particular application.
Storage: Purified monoclonal antibodies are generally stable for period at 4 0C. However repeated warming to room temperature and re-cooling may result in loss of activity and hence working titre.
FOR IN VITRO RESEARCH USE ONLY. NOT FOR USE IN OR ON HUMANS OR ANIMALS-NOT FOR DIAGNOSTICS. Not responsible for any Patent infringements with use or derivation of these products. Contains sodium azide (0.1%)- observe proper precautions.
CAT#: RDI-TRSFRNREC-AG $625.00/100ug $938.00/0.5mg $1250.00/1mg - Discontinued
Source: Human placenta. Blood sample from the tissue donor was tested and
found negative for HBsAg, HIV-1 ,HIV-2 and HCV antibodies.
Purification: Transferrin receptor was isolated from human placenta by modified method of Anderson G.J., MacKerras A., Powell L.W. and Halliday J.W(Biochim. Biophys. Acta 884, 225-233, 1986)
Presentation: Lyophilized from 50mM HEPES pH 7.4, 1M KCL, 0.1% CHAPS, 0.01% NaN3. It is recommended that the product should be reconstituted in distilled water.
Analysis: Purity more 95% according to SDS-PAGE
Immunological activity confirmed by reaction with monoclonals which are specific to human transferrin receptor.
Storage: At -20'C in lyophilized form. After reconstitution the solution should be aliquoted and stored at- 20'C. Avoid frequent freeze thaw cycles.
For In Vitro Research Use Only-Not for Use in or on human or animals-notfor use in diagnostics
see also other forms available cat#RDI-SCP1858 from human serum/plasma
see main antigen page
San Jose, 95123 CA Snell ave 658
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