rev:   June 22, 2007

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD70 Antibodies

Mouse anti-human CD70 antibodies

-2 clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-M1705clb              CLB-2F2                     ---            mIgG1      purified     $375.00

RDI-CD70abm-69          BU69                            V            mIgG1     see below Biotin/FITC/PE/other

Product Specification: mouse monoclonal anti-human CD70


Code: CLB-CD70


CAT#: RDI-M1705clb  $375.00/vial

Tests/vial: 200

Specificity: Cell type: Activated B and T lymfocytes.   Ag.MW.: 60 kD.

Reactivity: The monoclonal antibody is directed against the CD70-antigen,which is expressed on activated B and T lymphocytes. The monoclonal antibody also reacts positive with Sternberg-Reed cells.

Applications: Detection of activated B-and T-cells.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: 2F2. This clone has been derived from hybridization of SP2/0 cells with spleen cells of BALB/c mouse immunized with irradiated Ramos cells.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.

Source: Hybridoma culture supernatant.

Purification: Ammoniumsulphate precipitation and ion exchange chromatagraphy.

Packing: Each vial contains 1 ml with approximately 0.2mg/ml monoclonal antibody and 1 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18Deg C.Repeated thawing and freezing may reduce the antibody activity and should be avoided. Do not use this product beyond the stated expiration date.

Reference: 1. Hintzen R.,et al.,Journal of Immunology 152,1762 (1994).


FOR IN VITRO RESEARCH USE ONLY

Monoclonal anti-human CD70 (clone BU69)

cat# RDI-CD70abm-69   $312.00/100ug    

also available: Bulk no preservative/sterile filtered   cat#RDI-CD70abm-69XP   $1250.00/0.5mg

Presentation: 100ug in 0.1ml (1mg/ml) 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5   mg/ml Gentamicin Sulfate (as a preservative).

Clone: BU69

purification: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.

Isotype: Mouse IgG1

Immunogen: Human WM-1 (Waldenström's macroglobulinemia) cell line

INFORMATION: Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family. Antibody BU69 recognizes CD70 molecule of approximately 30 kd. Antibody BU69 inhibits T cell proliferation induced by dendritic cells.

References: R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773. M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.

STORAGE: Store at 2 - 5 oC. Freeze/Thawing is not   recommended.

PERFORMANCE: 500K cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul of anti-CD70 at a concentration of 20ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC , after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 0.88 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

For In Vitro Research Use Only

Also available:

non conjugated      cat#RDI-CD70abm-69      $312.00/100ug

FITC conjugated:  cat#RDI-CD70abm-69FT  $375.00/120T

Biotin conjugated: cat#RDI-CD70abm-69BT  $375.00/100ug

PE conjugated:       cat#RDI-CD70abm-69PE $500.00/100T

MOUSE MONOCLONAL ANTIBODY TO: HUMAN CD70

PRODUCT CODE    RDI-CBL 570    $375.00/200ug

CLONE      BU 69

ISOTYPE    IgG1

SOURCE     Mouse ascitic fluid

PURIFICATION METHOD      Protein A affinity chromatography

SPECIFICITY The antibody reacts with the 29 kDa moiety which is a type II transmembranous molecule of the TNF family. The antigen is expressed on a large proportion of activated B cells, a few activated T cells but not on resting lymphocytes, platelets or red cells.

APPLICATIONS *Flow cytometry*Immunocytochemistry on frozen sections

REFERENCES Leucocyte Typing V Oxford University Press (1995)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or th

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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