rev:   June 23, 2003

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD7 Antibodies

Mouse anti-human CD7 antibodies

CATALOG#                 CLONE#                Workshop  Host       Form        Price

RDI-M1339clb              CLB-3A1/1,7F3           IV         mIgG2a   purified     $375.00

RDI-M1567clb                " "                               "              "           FITC        $375.00

RDI-CD7abm-3A            3A1e                          --           mIgG2b    Purified

RDI-CD7abm-272          CD7-272                    --           mIgG1     tcs           $594.00/1ml  (paraffin sections)

-mIgG2b, recognizes a CD7 molecule of 40Kda

-suitable for flow cytometry,-unconjugated forms  immunoprecipitation and acetone fixed frozen sections

-clone 3A1e Mab induces T cell transmembrane calcium flux (Bulk quotes on request)

non conjugated       cat#RDI-CD7abm-3A     $312.00/100ug

FITC conjugated: cat#RDI-CD7abm-3AFT   $375.00/120T

Biotin conjugated: cat#RDI-CD7abm-3ABT  $375.00/100ug

PE conjugated: cat#RDI-CD7abm-3APE       $500.00/100T

Cell type : Human T lymphocytes

Reactivity: The monoclonal antibody is directed against the CD7-antigen (3A1-antigen), which is expressed on human T lymphocytes.The monoclonal antibody reacts with the cells of the T lymphocyte linage, with the cells of patients with a Lymphocytic Leukaemia of the T lymphocyte origin, and with the cells of patients with the Sezary Syndrome. Platelets and cells of the B lymphocyte myelocytic and monocytic lineage are found negative.

Applications: Monitoring of T-cell numbers in peripheral blood.Characterization of leukaemias and lymphomas.Identfication of T-cells in tissue.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-158 This clone has been derived from hybridization of SP2/0 cells with spleen cells of a CAF-1 mouse immunized with human T lymphocytes.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG2a.

Source: Ascites fluid of tumor bearing BALB/c mice

Purification: Ammoniumsulphate precipitation and ion exchange chromatography.

Packing: Each vial contains 1 ml monoclonal antibody and 10 mg BSA in 20 nM TRIS and 150 nM NaCI,pH 8.0.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18C.Repeated thawing and freezing may reduce the antibody activity and should be avoided. Do not use this product beyond the stated expiration date.
FOR IN VITRO RESEARCH USE ONLY

Cell Type : Human T lymphocytes.

Ag.MW. : 41 kD.

Reactivity: The monoclonal antibody is directed against the CD7-antigen (3A1-antigen), which is expressed on human T lymphocytes.The monoclonal antibody reacts with the cells of the T lymphocyte lineage, with the cells of patient s with a lymphocytic leukaemia of the T lymphocyte origin, and with the cells of patients with the Sezary Syndrome.Platelets and cells of the B lymphocyte myelocytic and monocytic lineage are found negative.

Methods: Direct immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-158 This clone has been derived from hybridization of SP2/0 cells with spleen cells of a CAF-i mouse immunized with human T lymphocytes.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG2a.

Source: Ascites fluid of tumour bearing BALB/c mice.

Purification: Ammoniumsulphate precipitation and ion exchangechromatography.

Chemical Characteristics:

Molecular F/P ratio : 7.0

Weight F/P ratio : 1.82x10-2

Fluorescein concentration : 1.82x10-4 mg/ml

Optical density ratio (E495/E280) at pH 7.2 : 1.28

Packing: Each vial contains 1 ml fluorescein conjugated monoclonal antibody and 10 mg BSA in PBS.

Preservative: Merthiolate (0.001%).

Storage: Monoconal antibodies should be stored 2-8 DEG C.

FOR IN VITRO RESEARCH USE ONLY

PRODUCT CODE : RDI-CBL502  $375.00/200ug vial
-also available FITC labeled, cat#RDI-CBL502FT    $375.00/100Tests

                          PE labeled cat#RDI-CBL502PE     $469.00/100Tests

CLONE: RFT-2a

ISOTYPE: IgG2a

SOURCE: Tissue Culture Supernatant

PURIFICATION METHOD: Protein A affinity chromatography

SPECIFICITY: This antibody reacts with teh CD7 antigen, a 40kDa polypeptide. this is the earliest marker antigen expressed in the T cell lineage and is found on T cell precursors in fetal liver and thorax prior to thymic colonization and in bone marrow and the thymus. It is also to be found on a small subpopulation of normal B cells and on malignant B cells.

Antigen distribution:

PHA stimulated blasts >95%

T Cells (E+) 88 +- 3%

Thymocytes 70 +-4%

Peripheral blood lymphocytes 65+- 3%

Monocytes <10%

Granulocytes <1%

B Cells (E-) <1%

APPLICATIONS: *Characetrization of leukaemias and lymphomas since the CD7 antigen is a marker for pluripotential stern cell leukaemias and T cell acute lymphocytic leukaemia.

-Flow cytometric analysis of CD7+ cells

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME order cat#RDI-CBL502-1XCP $1000.00/1mg

cat# RDI-CD7abm-272   $594.00/vial

Specificity: Human CD7 antigen

Clone: CD7-272

Ig Class: IgG1

Antigen: prokaryotic recombinant protein corresponding to a portion of the extracellular domain of CD7 molecule

Hybridoma partner: Mouse myeloma (p3-NS1-Ag4-1).

Preparation: Lyophilized tissue culture supernatant containing 15mM sodium azide. Reconstitute with 1ml of sterile distilled water.

USE: Effective on paraffin wax embedded tissue using 0.01M citrate buffer solution combined with the high temperature antigen unmasking technique

frozen sections:Not effective

Protocol: Immunohistochemistry:Typical working dilution 1:50 after high temperature antigen unmasking technique using 0.01M Citrate pH6.0. 60 Minutes incubation at 25'C. Standard ABC technique.
              -  Western blotting: 1:100 with chemiluminescence detection

Positive controls: tonsil

Staining pattern: Membrane

Storage and stability: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, aliquot in non frost freze freezer, Avoid repeated freeze thaw cycles. Prepare fresh working dilutions daily.

Stricly for in vitro research use only-Not for use in or on humans or animals-not for use in diagnostics

Sample High Temperature Unmasking Technqiue for Paraffin Sections: 0.01M sodium Citrate Buffer (pH 6.0)

1) Cut and mount sections on slides coated with "apes"

2) Deparrafinize sections and rehydrate to distilled water

3) Bring *or 0.01M sodium citrate buffer (pH 6.0) to a boil in a Prestige/Presto stainless steel pressure cooker, using a hot plate. Cover but do not lock lid.

-4) Position slides into metal staining racks and lower into pressure cooker ensuring slides are well immersed in buffer Lock the lid. The small valve will rise.

5) When the pressure indicator valve (the large one) has risen after about 4-5 minutes, Incubate sections for 1 minute.

6) Remove pressure cooker from heat source and run under cold water with lid on. When the small valve sinks open lid and remove slides and place immediately into distilled water. DO NOT OPEN LID UNTIL THE SMALL VALVE Sinks or Vent Cover Lock Drops (see manual with your particular unit).

7) Wash sections PBS buffer (10mM phosphate, 0.15M NaCl pH 7.5) for 1 X 5 minutes (or 100mM TRIS, 0.15M NaCl, pH 7.5)

8) Place sections in 1.5% hydrogen peroxide/methanol for 10 minutes (or use other appropriate peroxidase blocking procedure).

9) Wash sections in distilled water for 2 X 5 minutes, then wash sections in PBS buffer for 2 X 5 minutes.

10) Place sections in normal serum for 20 minutes.

11) Cover sections with primary antibody. (Optimum dilution, incubation time and temperature must be determined for each antibody and in each lab).

12) Wash in PBS buffer for 2 X 5 minutes

13) Incubate sections in secondary antibody for 30 minutes

14) Wash in PBS buffer for 2 X 5 minutes

15) Incubate slides in ABC complex for 30 minutes

16) Wash in PBS buffer for 2 X 5 minutes

17) Incubate slides in DAB or other suitable peroxidase substrate

18) Wash in distilled water for 2 X 5 minutes

19) Counterstain with haematoxylin (if required), dehydrate, coverslip, and mount

Material:

APES (3-aminopropyltriethoxysilane) cat#A3648 Sigma

To avoid sections becoming detached, sections should be mounted on "APES" covered slides, then dried at 37 DEG C overnight followed by drying at 56 DEG C for 60 minutes.

0.01M Sodium Citrate Buffer (ph 6.0): Add 2.94g of tri-sodium citrate to 1 liter of distilled water. Adjust pH 6 using 1.0M HCL.

Tris: 10mmM Tris, 0.15M NaCl, pH 7.5.

PBS: 10mM Phosphate, 0.15M MaCl pH 7.5

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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