rev: 5/16/2008
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NOTE: Due to patent fees that would exceede research sales of the
anti-human CD62P, we regret we cannot offer anti-human
CD62P monoclonal antibodies for sale in the
CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD62P
Mouse anti-human CD62P antibodies
-3 clones
CATALOG# CLONE# Workshop Host Form Price
RDI-M1543clb CLB-trom/6(42),C2 IV
mIgG1 purified $412.50
new cat#: 10R-CD62PaHU
RDI-M1706clb " " " " PE $469.00
RDI-CBL469FT " " -- " FITC $438.00
RDI-CBL469PE " " -- " PE $500.00
RDI-CD62Pabr2 rabbit polyclonal --- --- purified $440.00 (multi-species)
NOTE: Due to patent fees that would exceede research
sales of the anti-human CD62P, we regret we cannot
offer anti-human CD62P monoclonal antibodies for sale in the
Product Specification:
mouse monoclonal anti-human CD62P
PeliCluster CD62P
cat#RDI-M1543clb, new cat#: 10R-CD62PaHU
Test/vial 200
Clone CLB-tromb/6, C2
This clone has been derived from hybridization of SP2/0 Ag14 cells with spleen
cells of a (BALB/c x A/J) mouse immunized with human platelets. This antibody
has been clustered to CD62P in the fifth international Workshop on Human White
Cell differentiation Antigens in
Isotype Mouse IgG1.
Source Ascites fluid of tumour bearing BALB/c mice.
Purification Ammoniumsulphate precipitation followed by affinity
chromatography.
Packing Each vial contains 1 ml with approximately 0.2
mg/ml monoclonal antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH
8.0.
Preservative Mertiolate (0.001%).
Storage and stability Monoclonal antibodies should be stored in the dark at
2-8°C. The reagent is stable until the expiry date stated on the vial
label.
Major reactivity The monoclonal antibody is directed
against the CD62P- antigen (GMP-140 or PADGEM), which is expressed in platelet
alpha granules, which is translocated to the platelet surface upon activation
with strong agonists. A similar protein, derived from endothelial cell Weibel-
Palade bodies, is transiently expressed on the surface of activated endothelial
cells.
Molecular mass Apparent molecular weight under
reducing conditions: 140 kDa.
Application
Methods Indirect immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.
References 1. Modderman, P.W., Knapp, W. et al. (editors), Leucocyte Typing IV, 1038.
APPLICATION FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE
Reagents
- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).
Remove aggregates by centrifugation at 1000 x g for 10 minutes.
- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).
- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.
- Microtiter plates (96 wells, V bottom) or tubes.
Procedure
1 Fixate the cells with PFA 1% and wash them with buffer.
2 Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.
3 Add 45 µl of cell suspension to the microtiter wells or tubes.
4 Add 5 µl monoclonal antibody to the microtiter wells or tubes and mix gently.
5 Incubate for 30 minutes at 2-8 C.
6 Add 100 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
7 Aspirate the supernatant from the cell pellet and resuspend the cells.
8 Add 200 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
9 Aspirate the supernatant from the cell pellet and resuspend the cells.
10 Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human immunoglobulins, diluted 1:80.
11 Incubate for 30 minutes at 2-8 C.
12 Repeat step 6-9.
13 Add 200 µl buffer.
14 The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.
NOTE: Care should be taken when drawing blood to avoid activation of platelets.
Platelets activation markers are weakly expressed on platelets prepared from
directly fixed normal whole blood. The expression is maximal on thrombin (1
U/ml) stimulated washed platelets.
FOR IN VITRO RESEARCH USE ONLY
PeliCluster CD62P PE
cat#RDI-M1706clb $469.00/vial
Test/vial 100
Clone CLB-tromb/6, C2
This clone has been derived from hybridization of SP2/0 Ag14 cells with spleen
cells of a (BALB/c x A/J) mouse immunized with human platelets. This antibody
has been clustered to CD62P in the fifth international Workshop on Human White
Cell differentiation Antigens in
Isotype Mouse IgG1.
Source Ascites fluid of tumour bearing BALB/c mice.
Purification Ammoniumsulphate precipitation followed by affinity
chromatography.
Conjugation Conjugated with R-Phycoerythrin (PE).
Molecular F/P ratio between 1.0 - 2.0.
Packing Each vial contains 1 ml PE conjugated
monoclonal antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.
Preservative Mertiolate (0.001%).
Storage and stability Monoclonal antibodies should be stored in the dark at
2-8°C. The reagent is stable until the expiry date stated on the vial
label.
Major reactivity The monoclonal antibody is directed
against the CD62P- antigen (GMP-140 or PADGEM), which is expressed in platelet
alpha granules, which is translocated to the platelet surface upon activation
with strong agonists. A similar protein, derived from endothelial cell Weibel-
Palade bodies, is transiently expressed on the surface of activated endothelial
cells.
Molecular mass Apparent molecular weight under
reducing conditions: 140 kDa.
Application Detection of activated platelets.
Thrombus imaging.
Methods Indirect immunofluorescence staining with analysis by
flowcytometry or fluorescence microscopy.
References 1. Modderman, P.W., Knapp, W. et al.
(editors), Leucocyte Typing IV, 1038.
For In Vitro Research Use Only
DATA SHEET: rabbit anti-CD62P (P-Selectin) Human/Mouse/Rat + (Discontinued)
Catalog#: RDI-CD62Pabr $438.00
Package Size: 100ug purified ab w/0.05%NaN3
Species: rabbit
Reactivity: reacts with CD62P (P-Selectin) in human, mouse, and rat. Also reported to react with baboon, sheep, cattle, dog and hamster.
Antigen: purified P-Selectin from human platelets
Uses: -western blotting,
-histochemistry (acetone fixed frozen and section and paraffin embedded sections (tested on mouse and human)
-antibody
reported to block the function of P-selectin
-immunofluorescent staining
-flow cytometry(recommend specific anti-human and anti-mouse monoclonals for human and mouse flow use)
Storage: Store at -20 DEG C. For extensive dilutions, add protein containing or
stabilizing medium. Avoid frequent freeze thaw cycles.
ref: J. Biol Chem 266:5371-5374
J. Exp Med 175:1147-1150
Transplant Proc 25:2930-2931
J. Exp Med 179:329-334
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. Sodium azide may form explosive compounds in
presence of heavy metals or under acidic conditions. Flush drains with copious
amounts of water to prevent buildup of explosive compounds.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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