rev: June 16, 2003

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD62L Antibodies

Mouse anti-human CD62L  antibodies

-1 clone

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-LECAM1               Dreg56                          V             mIgG1       purified    $500.00

RDI-CBL523                 FMC46                         V            mIgG2b      purified    $375.00 

RDI-CD62Labm-11         LAM1-116                   -            mIgG2ak     purified/FITC/PE/Biotin

clone LAM1-116

cat#RDI-CD62Labm-11     $312.00/100ug

cat#RDI-CD62Labm-1BT  $375.00/100ug Biotin

cat#RDI-CD62Labm-1FT  $375.00/120T FITC

cat#RDI-CD62Labm-1PE  $500.00/120T PE

PRODUCT CODE RDI-CBL523      $375.00/vial 200ug

Also available: FITC labeled cat#RDI-CBL523FT   $375.00/100 tests

                           PE labeled cat#RDI-CBL523PE   $469.00/100 tests

CLONE       FMC 46

ISOTYPE     IgG2b

SOURCE   Mouse ascitic fluid

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY

This antibody recognizes CD62L, known as L-selectin, a 76 kDa molecule which is a member of the selectin family of adhesion receptors. These function in leucocyte binding to activated endothelium and in lymphocyte homing to high endothelial venules. The antigen is expressed on neutrophils, monocytes, T and B lymphocyte subsets and NK cells. CD62L on neutrophils, but not on lymphocytes, carries the carbohydrate structure sialyl-Lewis X. This carbohydrate is ligand for the selectins P-selectin (CD62P) and E-selectin (CD62E) which can be expressed on endothelial cells, and the interaction of CD62L with these selectins has been argued to play a role in the initial adhesion of neutrophils to endothelium.

The epitope is expressed at high density on the majority of CD45RA-positive and CD45RO-positive peripheral blood T cells (60-70%) and on a subset of thymocytes that includes the multinegative CD3-negative, CD4-negative, and CD8-negative progenitor cells and the CD45RO-negative presumptive thymic generative lineage. It appears at reduced density and frequency on CD45RA-negative thymocytes, comprised mainly of death-committed cells. The antibody also labels a major subset of peripheral blood B cells and thymic B cells.

APPLICATIONS

* The antibody can be used in regulation studies to determine initiation of the inflammatory response whereby the binding of lymphocytes to endothelium and the rolling of leucocytes at sites of tissue injury are mediated. L-selectin expression is downmodulated by phorbol myristate acetate (PMA) stimulation of lymphocytes and neutrophils. Strong staining can be observed in splenic follicles and round blood vessels and also the thymic medulla, subcapsular areas, and the mantle zone of germinal centres of the lymph node are stained.

* Flow cytometry procedures and immunohistochemistry where reactivity is mainly restricted to lymphocytes in a differential expression pattern

REFERENCES

Leucocyte Typing V, Oxford University Press (1995)

Pilarski, L. M. et al. J. Immunol. 147, 136-43 (1991)

Tedder, T.F. Penta, A.C. Levine, H.B. Freedman, A.S. J. Immunol 144, 532-40 (1990)

Spertini, O. Kansas, G.S. Munro, J.M. Griffin, J.D. Tedder, T.F. Nature 349, 691-4 (1991)

Picker, L.J. Warnock, R.A. Burns, A.R. Doerschuk, C.M. Berg, E.L. Butcher, E.C. Cell 66, 921-33 (1991)

LOT SPECIFIC DATA

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Bulk, milligram (NO BSA, no AZIDE) cat#RDI-CBL523-1XP $1062.00/mg

Catalog#: RDI-LECAM1

Package Size: 200ug purified, sterile, preservative, carrier free in 200ul PBS buffer

BULK:  cat# RDI-CD62Labm-56XP $750.00/500ug in 0.5ml (no azide)

also available:

cat#RDI-CD62Labm-56 $281.00 100ug in 0.2ml with 0.09% NaN3

cat#RDI-CD62Labm-5FT $469.00/100tests FITC conjugated

cat#RDI-CD62Labm-5PE $500.00/100tests PE conjugated

Species: mouse IgG1

LOT# see batch sheet

CLONE: DREG-56

Activity: Reacts with human 80-85 kD glycoprotein LECAM-1. Blocks >90% of lymphocyte binding to high endothelial venules (HEV) in peripheral lymphoid tissue, but not mucosal HEV, also inhibits lymphocyte binding of PPME.

Application: Indirect immunofluorescence cell surface staining. Inhibition of human lymphocyte binding to peripheral lymph node HEV. Immunoprecipitation, Western blot.

Reactivity: Lymphocytes and granulocytes

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

REF: Blood,78(3):805-811.1991

It is the responsibility of the user to comply with all local/stateand Federal rules in the use of this product. We are not responsiblefor any patent infringements that might result with the use of or derivation of this product.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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