rev: September 14, 2007

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD61 Antibodies


Mouse anti-human CD61  antibodies

-2 clones    & 1 polyclonal


CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-M1355clb              CLB-trom/1, C17          IV            mIgG1       purified    $375.00

RDI-M1592clb                   " "                              "                 "             FITC      $375.00

RDI-CBL479                 PM6/13                         V             mIgG1       purified    $375.00

RDI-CBL479FT                 " "                              "                 "            FITC       $375.00

RDI-CBL479PE                 " "                              "                 "            PE           $469.00

RDI-CD61abr               rabbit polyclonal            --                ---         100ul       $345.00


Product Specification: mouse monoclonal and human CD61

PeliCluster  CD61

cat#RDI- M1355clb   $375.00/vial

Test/vial 200

Clone CLB-thromb/1, C17
This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human platelets. This antibody has been clustered to CD61 in the fourth international Workshop on Human White Cell
differentiation Antigens

Isotype Mouse, IgG1.

Source Ascites fluid of tumour bearing BALB/c mice

Purification Ammoniumsulphate precipitation and ion exchange chromatography.

Packing Each vial contains 1 ml with approximately 0.2 mg/ml monoclonal antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.

Preservative Mertiolate (0.001%).

Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD61- antigen (GP IIIa), which is expressed on human platelets. The monoclonal antibody reacts with platelets, megakaryocytes, megakaryoblasts, endothelial cells, fibroblasts, smooth muscle cells and osteoblasts (integrin beta-3 chain) in complexed form and does not react with the platelets of patients with Glanzmann Thrombasthenia.

Molecular mass Apparently molecular weight under reducing conditions: 110 kDa.

Application The monoclonal antibody is a useful marker of megakaryoblasts and megakaryoblastic leukaemias.

The monoclonal antibody inhibits binding of ligand (fibrinogen, fibronectin, etc.).

Methods Indirect immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.


References

1. Tetteroo, P.A.T. et al., Brit. J. Haematol., 55, 509 (1983).

2. Vinci, G. et al., Brit. J. Haematol., 56, 589 (1984).

3. Leeksma, O.C. et al., Blood, 67, 1176 (1986).

4. Giltay, J.C. et al., Blood, 69, 809 (1987).

5. Borne, A.E.G.Kr. von dem, Leucocyte Typing III, 748 (1987).

6. Modderman, P.W. et al., Trombosis and Haemostasis, 60, 68 (1988).

7. Knapp, W. et al., Immunology Today 10, 253 (1989).



APPLICATION  FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE


Reagents

- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).

  Remove aggregates by centrifugation at 1000 x g for 10 minutes.

- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).

- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.

- Microtiter plates (96 wells, V bottom) or tubes.


Procedure

1   Fixate the cells with PFA 1% and wash them with buffer.

2   Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.

3   Add 45 µl of cell suspension to the microtiter wells or tubes.

4   Add 5 µl monoclonal antibody to the microtiter wells or tubes and mix gently.

5   Incubate for 30 minutes at 2-8 C.

6   Add 100 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

7   Aspirate the supernatant from the cell pellet and resuspend the cells.

8   Add 200 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

9   Aspirate the supernatant from the cell pellet and resuspend the cells.

10  Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human                                  immunoglobulins, diluted 1:80.

11  Incubate for 30 minutes at 2-8 C.

12  Repeat step 6-9.

13  Add 200 µl buffer.

14  The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.


NOTE: Care should be taken when drawing blood to avoid activation of platelets.

Platelets activation markers are weakly expressed on platelets prepared from directly fixed normal whole blood.

The expression is maximal on thrombin (1 U/ml) stimulated washed platelets.


FOR IN VITRO RESEARCH USE ONLY


mouse monoclonal CD61, conjugated to Fluorescein   PeliCluster CD61 F

cat#RDI-M1592clb

Test/vial 100

Clone CLB-thromb/1, C17

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human platelets. This antibody has been clustered to CD61 in the fourth international Workshop on Human White Cell differentiation Antigens

Isotype Mouse, IgG1.

Source Ascites fluid of tumour bearing BALB/c mice.

Purification Ammoniumsulphate precipitation and ion exchange chromatography.

Conjugation Conjugated with fluorescein iso thiocyanate isomer 1 (FITC).

Molecular F/P ratio between 5.5 - 9.5.

Packing Each vial contains 1 ml FITC conjugated monoclonal antibody and 10 mg BSA in PBS.

Preservative Mertiolate (0.001%).

Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD61- antigen (GP IIIa), which is expressed on human platelets. The monoclonal antibody reacts with platelets, megakaryocytes, megakaryoblasts, endothelial cells, fibroblasts, smooth muscle cells and osteoblasts (integrin beta-3 chain) in complexed form and does not react with the platelets of patients with Glanzmann Thrombasthenia.

Molecular mass Apparently molecular weight under reducing conditions: 110 kDa.

Application The monoclonal antibody is a useful marker of megakaryoblasts and megakaryoblastic leukaemias.

The monoclonal antibody inhibits binding of ligand (fibrinogen, fibronectin, etc.).

Methods Direct immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.


References

1. Tetteroo, P.A.T. et al., Brit. J. Haematol., 55, 509 (1983).

2. Vinci, G. et al., Brit. J. Haematol., 56, 589 (1984).

3. Leeksma, O.C. et al., Blood, 67, 1176 (1986).

4. Giltay, J.C. et al., Blood, 69, 809 (1987).

5. Borne, A.E.G.Kr. von dem, Leucocyte Typing III, 748 (1987).

6. Modderman, P.W. et al., Trombosis and Haemostasis, 60, 68 (1988).

7. Knapp, W. et al., Immunology Today 10, 253 (1989).

For research only! Not for human use! M1592\01 0708960858


FOR IN VITRO RESEARCH USE ONLY


Mouse ANTI-Human CD61 ANTIGEN

PRODUCT CODE :RDI-CBL479      $375.00/vial 200ug

Also available FITC labeled:cat#RDI-CBL479FT $375.00/100 Tests

                          PE labeled:cat#RDI-CBL479PE $469.00/100 tests

CLONE: PM 6/13

ISOTYPE: IgG1

SOURCE: Tissue culture supernatent.

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: The antibody is specific to the 110kD platelet glycoprotein gp IIIa and inhibits platelet aggregation and activation induced by thrombin and collagen. The platelet glycoprotein is the integrin ß3 chain of the vitronectin receptor and GP IIb/IIIa. The antigen is present on platelets and megakaryocytes.

Antigen distribution: Platelets > 98%

Megakaryocytes > 98%

Granulocytes 0

Monocytes 0

LGL cells 0

B-cells 0

T-cells 0

Peripheral blood lymphocytes 0

APPLICATIONS:

* Marker for megakaryoblastic/cytic leukaemias and idiopathic thrombocythemia.


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200µg/2ml in phosphate buffered saline solution containing 10mM sodium azide and 1mg/ml BSA. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

 

Bulk, NO BSA/NO AZIDE 1mg order cat#RDI-CBL479-1XP $1062.00/mg

                         Larger sizes quoted on request-Bulk inquiries Welcome.


DATA SHEET: rabbit anti-INTEGRIN Beta 3 (CD61)

Catalog#: RDI-CD61abr     $345.00    $250.00/package 2+

Package Size: 100ul neat sera with 0.02% NaN3

Species: rabbit

Immunogen: Integrin Beta 3 protein purified from human platelets by lectin chromatography and electroelution from SDS-PAGE

Reactivity: reacts with Beta 3 integrin. Specificity determined by immunoprecipitation from a detergent extract of S(35) methionine labeled endothelial cells. No cross- reactivity to beta 1 or beta 2 integrins

Species Reactivity: human,porcine, horse, sheep and goat (not with bovine or mouse)

Uses: -Western blot 1:100

           -Elisa: 1:1000

           -immunoprecipitation: approx 5ul/5Million cells to precipitate AV/B3

            -immunohistochemistry:1:80 on acetone fixed tissue only

Titers must be optimized for each   application

ref: Exp Cell Res, 189:100-108 (1990)

       Science 238:491(1987)

Storage: Store at -20 DEG C in undiluted aliquots. For extensive dilutions, add protein containing or stabilizing medium. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or   on humans or animals or for diagnostics.

Note: Upon receipt, tighten cap, and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in vial (during shipping, some antibody can become lodged inside the cap and on sides of vial.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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