rev: September 14, 2007
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD61 Antibodies
Mouse anti-human CD61 antibodies
-2 clones & 1 polyclonal
CATALOG# CLONE# Workshop Host Form Price
RDI-M1355clb CLB-trom/1, C17 IV mIgG1 purified $375.00
RDI-M1592clb " " " " FITC $375.00
RDI-CBL479 PM6/13 V mIgG1 purified $375.00
RDI-CBL479FT " " " " FITC $375.00
RDI-CBL479PE " " " " PE $469.00
RDI-CD61abr rabbit polyclonal -- --- 100ul $345.00
Product Specification:
mouse monoclonal and human CD61
PeliCluster CD61
cat#RDI- M1355clb $375.00/vial
Test/vial 200
Clone CLB-thromb/1, C17
This clone has been derived from hybridization of SP2/0 cells with spleen cells
of a BALB/c mouse immunized with human platelets. This antibody has been
clustered to CD61 in the fourth international Workshop on Human White Cell
differentiation Antigens
Isotype Mouse, IgG1.
Source Ascites fluid of tumour
bearing BALB/c mice
Purification Ammoniumsulphate precipitation and ion
exchange chromatography.
Packing Each vial contains 1 ml with approximately 0.2
mg/ml monoclonal antibody and 10 mg BSA in 20 mM
TRIS and 150 mM NaCl, pH
8.0.
Preservative Mertiolate (0.001%).
Storage and stability Monoclonal antibodies should be stored in the dark at
2-8°C. The reagent is stable until the expiry date stated on the vial
label.
Major reactivity The monoclonal antibody is directed
against the CD61- antigen (GP IIIa), which is
expressed on human platelets. The monoclonal antibody reacts with platelets, megakaryocytes, megakaryoblasts,
endothelial cells, fibroblasts, smooth muscle cells and osteoblasts
(integrin beta-3 chain) in complexed
form and does not react with the platelets of patients with Glanzmann
Thrombasthenia.
Molecular mass Apparently molecular weight under
reducing conditions: 110 kDa.
Application The monoclonal antibody is a useful marker of megakaryoblasts
and megakaryoblastic leukaemias.
The monoclonal antibody inhibits binding of ligand
(fibrinogen, fibronectin, etc.).
Methods Indirect immunofluorescence
staining with analysis by flowcytometry or
fluorescence microscopy.
References
1. Tetteroo, P.A.T. et al., Brit. J. Haematol., 55, 509 (1983).
2. Vinci, G. et al., Brit. J. Haematol., 56, 589 (1984).
3. Leeksma, O.C. et al., Blood, 67, 1176 (1986).
4. Giltay, J.C. et al., Blood, 69, 809 (1987).
5. Borne, A.E.G.Kr. von dem, Leucocyte Typing III, 748 (1987).
6. Modderman, P.W. et al., Trombosis and Haemostasis, 60, 68 (1988).
7. Knapp, W. et al., Immunology Today 10, 253 (1989).
APPLICATION FOR AN INDIRECT IMMUNOFLUORESCENCE
TECHNIQUE
Reagents
- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).
Remove aggregates by centrifugation at 1000 x g for 10 minutes.
- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).
- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.
- Microtiter plates (96 wells, V bottom) or tubes.
Procedure
1 Fixate the cells with PFA 1% and wash them with buffer.
2 Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.
3 Add 45 µl of cell suspension to the microtiter wells or tubes.
4 Add 5 µl monoclonal antibody to the microtiter wells or tubes and mix gently.
5 Incubate for 30 minutes at 2-8 C.
6 Add 100 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
7 Aspirate the supernatant from the cell pellet and resuspend the cells.
8 Add 200 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
9 Aspirate the supernatant from the cell pellet and resuspend the cells.
10 Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human immunoglobulins, diluted 1:80.
11 Incubate for 30 minutes at 2-8 C.
12 Repeat step 6-9.
13 Add 200 µl buffer.
14 The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.
NOTE: Care should be taken when drawing blood to avoid activation of platelets.
Platelets activation markers are weakly expressed on platelets prepared from
directly fixed normal whole blood.
The expression is maximal on thrombin (1 U/ml) stimulated washed platelets.
FOR IN VITRO RESEARCH USE ONLY
mouse monoclonal CD61,
conjugated to Fluorescein PeliCluster
CD61 F
cat#RDI-M1592clb
Test/vial 100
Clone CLB-thromb/1, C17
This clone has been derived from hybridization of SP2/0 cells with spleen cells
of a BALB/c mouse immunized with human platelets. This antibody has been
clustered to CD61 in the fourth international Workshop on Human White Cell
differentiation Antigens
Isotype Mouse, IgG1.
Source Ascites fluid of tumour
bearing BALB/c mice.
Purification Ammoniumsulphate
precipitation and ion exchange chromatography.
Conjugation Conjugated with fluorescein iso thiocyanate isomer 1 (FITC).
Molecular F/P ratio between 5.5 - 9.5.
Packing Each vial contains 1 ml FITC conjugated
monoclonal antibody and 10 mg BSA in PBS.
Preservative Mertiolate (0.001%).
Storage and stability Monoclonal antibodies should be stored in the dark at
2-8°C. The reagent is stable until the expiry date stated on the vial
label.
Major reactivity The monoclonal antibody is directed
against the CD61- antigen (GP IIIa), which is
expressed on human platelets. The monoclonal antibody reacts with platelets, megakaryocytes, megakaryoblasts,
endothelial cells, fibroblasts, smooth muscle cells and osteoblasts
(integrin beta-3 chain) in complexed
form and does not react with the platelets of patients with Glanzmann
Thrombasthenia.
Molecular mass Apparently molecular weight under
reducing conditions: 110 kDa.
Application The monoclonal antibody is a useful marker of megakaryoblasts
and megakaryoblastic leukaemias.
The monoclonal antibody inhibits binding of ligand
(fibrinogen, fibronectin, etc.).
Methods Direct immunofluorescence
staining with analysis by flowcytometry or
fluorescence microscopy.
References
1. Tetteroo, P.A.T. et al., Brit. J. Haematol., 55, 509 (1983).
2. Vinci, G. et al., Brit. J. Haematol., 56, 589 (1984).
3. Leeksma, O.C. et al., Blood, 67, 1176 (1986).
4. Giltay, J.C. et al., Blood, 69, 809 (1987).
5. Borne, A.E.G.Kr. von dem, Leucocyte Typing III, 748 (1987).
6. Modderman, P.W. et al., Trombosis and Haemostasis, 60, 68 (1988).
7. Knapp, W. et al., Immunology Today 10, 253 (1989).
For research only! Not for human use! M1592\01 0708960858
FOR IN VITRO RESEARCH USE ONLY
Mouse ANTI-Human CD61 ANTIGEN
PRODUCT CODE :RDI-CBL479 $375.00/vial 200ug
Also available FITC labeled:cat#RDI-CBL479FT $375.00/100 Tests
PE labeled:cat#RDI-CBL479PE $469.00/100
tests
CLONE: PM 6/13
ISOTYPE: IgG1
SOURCE: Tissue culture supernatent.
PURIFICATION METHOD: Protein A affinity
chromatography.
SPECIFICITY: The antibody is specific to the 110kD platelet glycoprotein gp IIIa and inhibits platelet
aggregation and activation induced by thrombin and collagen. The platelet
glycoprotein is the integrin ß3 chain of the vitronectin receptor and GP IIb/IIIa.
The antigen is present on platelets and megakaryocytes.
Antigen distribution: Platelets > 98%
Megakaryocytes > 98%
Granulocytes 0
Monocytes 0
LGL cells 0
B-cells 0
T-cells 0
Peripheral blood lymphocytes 0
APPLICATIONS:
* Marker for megakaryoblastic/cytic leukaemias and idiopathic thrombocythemia.
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration
of 200µg/2ml in phosphate buffered saline solution containing 10mM sodium
azide and 1mg/ml BSA. We recommend that each
laboratory determine an optimum working titre for use
in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods
at 4oC. However repeated warming to room temperature and re-cooling may result
in a loss of activity and hence effective working titre.
It is recommended that monoclonal antibodies are aliquotted
upon receipt and then stored at -20C. After thawing they should then be used as
a stock solution for up to 30 days. Dilute working solutions are best prepared
on the day of use.
For research use only. Not supplied for use in human
diagnostic or therapeutic procedures
Bulk, NO BSA/NO AZIDE 1mg order cat#RDI-CBL479-1XP $1062.00/mg
Larger sizes quoted on request-Bulk inquiries Welcome.
DATA SHEET: rabbit anti-INTEGRIN Beta 3 (CD61)
Catalog#: RDI-CD61abr $345.00 $250.00/package
2+
Package Size: 100ul neat sera with 0.02% NaN3
Species: rabbit
Immunogen: Integrin Beta
3 protein purified from human platelets by lectin
chromatography and electroelution from SDS-PAGE
Reactivity: reacts with Beta 3 integrin. Specificity
determined by immunoprecipitation from a detergent
extract of S(35) methionine
labeled endothelial cells. No cross- reactivity to beta 1 or beta 2 integrins
Species Reactivity: human,porcine,
horse, sheep and goat (not with bovine or mouse)
Uses: -Western blot 1:100
-Elisa: 1:1000
-immunoprecipitation: approx 5ul/5Million cells to precipitate AV/B3
-immunohistochemistry:1:80 on acetone fixed tissue only
Titers must be optimized for each application
ref: Exp Cell Res, 189:100-108 (1990)
Science 238:491(1987)
Storage: Store at -20 DEG C in undiluted aliquots. For extensive dilutions, add protein containing or stabilizing medium. Avoid frequent freeze thaw cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Note: Upon receipt, tighten cap, and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in vial (during shipping, some antibody can become lodged inside the cap and on sides of vial.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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