rev:  July 28, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD6 Antibodies


Mouse anti-human CD6 antibodies

-2 clones     Mem98(purified, FITC & PE)

                   RFT-12 (purified & FITC) 


CATALOG#                 CLONE#                Workshop  Host     Form        Price

RDI-CBL165                  Mem98                      --           mIgG1    purified    $438.00

RDI-CBL165FT              " " "                           --             "           FITC       $438.00

RDI-CBL165PE               " " "                          --             "           PE           $500.00

RDI-CBL504                   RFT-12 *                    --         mIgM     purified    $375.00

RDI-CBL504FT                " " "                          --             "          FITC       $375.00

   *cytolytic complement fixing clone  


MOUSE ANTI-HUMAN CD6 ANTIGEN

PRODUCT CODE :RDI-CBL165    $438.00/vial 200ug

CLONE: MEM 98

ISOTYPE: IgG1

SOURCE: Mouse ascitic fluid

PURIFICATION METHOD: hydrophobic interaction chromatography

SPECIFICITY: CD6 antigen is a 120kDa molecular weight single chain membrane glycoprotein found on > 80% of mature T-cells with weak expression on corticol thymocytes, also present on the majority of chronic B-lymphocytic leukaemias.

Antigen distribution: T-cells (E+) > 80%

                               Peripheral blood lymphocytes 74 ± 6%

                               Thymocytes 25 ± 9%

                               B-cells (Ig+) 10 ± 5%

                               Monocytes < 1%

                               Granulocytes < 1%

                               NK cells (CD16+) <1%


APPLICATIONS:

* Identification by FACS of thymocytes and B-CLL cells.

* Identification of a major T-cell subset and B-cell subset by immunohistochemistry.

* Immunosuppressive therapy.


REFERENCES: Basil,V. et al. Fol. Biol. (Prague) 35, 289-98. (1989)

                          Aruffo, A. et al, J. Exp. Med 174, 949-952 (1991)


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Also available, clone RFT-12, mIgM which will lyse target cell in the presence of complement, order cat#RDI-CBL504 $375.00/vial /200ug in 2ml vial (+1mg/ml BSA + 10mm NaN3)

for azide and BSA free order cat#RDI-CBL504-1XP  $1000.00/milligram


ANTI-Human CD6 ANTIGEN


PRODUCT CODE :RDI-CBL504   $375.00/vial 200ug


CLONE: RFT-12

ISOTYPE: IgM

SOURCE: Tissue culture supernatant


PURIFICATION METHOD: ammonium sulphate precipitation followed by gel filtration.


SPECIFICITY: The antibody reacts with a 120 kDa single chain membrane glycoprotein specific for the CD6 antigen. the anigen is found on >80% normal mature T cells and a sub-population of B cells in peripheral blood. The antigen also exhibits weak expression on cortical thymocytes and the majority of chronic B lymphocytic leukaemias.

Antigen Distribution:

T cells(E+)                               >80%

Peripheral Bllod lymphocyte       74 +- 6%

Thymocytes                                25 +- 9%

B Cells (Ig+)                              10 +- 5%

Monocytes                                 <1%

Granulocytes                               <1%

NK Cells (CD16+)                     <1%



APPLICATIONS:

* Identification by Facs of thymocytes and B-CLL cells.

* Identification of a major T cell subset and B cell subset by histochemistry

* Immunosuppresive therapy

-Lysis of CD6+ cells in the presence of complement


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -200C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME order cat#RDI-CBL504-1XCP $1000.00/1mg

                                        $812.00/mg 2-10

                                        $625.00/mg 11-50mg

Also available cytolytic abs for CD6,CD7, Cd8, CD10, CD19 , CD37 & CD105 same pricing


Goat anti-CD6 HUMAN

cat#RDI-CD6Cabg    $406.00/200ug vial

-goat polyclonal antibody IgG

-epitope mapping at amino acids mapping at the carboxy terminus of the precursor form of CD6 of human origin

-specific for CD6 of human origin

-suitable for western blotting and immunohistochemistry

control/blocking peptide available: cat#RDI-CD6C-CP $125.00/100ug in 0.5ml +NaN3 & 100ug BSA $94.00 with antibody

Goat anti-CD6 Mouse/Rat

cat#RDI-MCD6Cabg    $406.00/200ug vial

-goat polyclonal antibody IgG

-epitope mapping at amino acids mapping at the carboxy terminus of the precursor form of CD6 of mouse origin

-specific for CD6 of mouse and rat origin

-suitable for western blotting and immunohistochemistry

control/blocking peptide available: cat#RDI-MCD6C-CP $125.00/100ug in 0.5ml +NaN3 & 100ug BSA $94.00 with antibody

Mouse anti-CD6       HUMAN/Mouse

cat#RDI-CD6abm-L14    $406.00/200ug vial

-mouse monoclonal antibody IgG1

-clone ref: SPV-L14

-specific for CD6 of human and mouse origin

-suitable for western blotting (tested on lysates of BJAB, RAMOS, Molt-4 and CLL-2) and immunohistochemistry

-Also available FITC labeled for flow cytometry, cat#RDI-CD6abm-LFT $438.00/200ug


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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