rev:  October 3, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD59 Antibodies

Mouse anti-human CD59  antibodies

-3 clones  

New Reference for use of CD55 and CD59:

NOTE:You might be interested to know that about a dual labelled protocol for flow cytometry studies of paroxysmal nocturnal haemoglobinurea uses CD59-FITC and CD55PE (our clones MEM43 & 143-30). The authors indicated that the low prevalence of the CD55 required the PE label to be used with that antibody.


The published reference of interest on the CD59FITC/CD55PE is: Practical Haematology (1994) Chapter 15 by Luzzatto & Hillman"Laboratory methods used in the investigation of paraxysomal nocturnal haemoglobinuria".

CATALOG#                 CLONE#                  Workshop        Host         Form        Price

RDI-CBL467                  MEM 43                      IV + V        mIgG2a    purified    $438.00

RDI-CBL467FT                " "                               "                 "               FITC       $440.00

RDI-CBL467BT                " "                               "                 "               Biotin      $438.00

RDI-CBL467PC5              " "                               "                 "             R-PE/CY5  $500.00

RDI-CBL547PE                                                                                      PE             $469.00

RDI-CBL547PC5                                                                                    RPE-CY5  $469.00

RDI-CBL547BT                                                                                     Biotin         $375.00

RDI-CD59-P282            P282                             V                "               purified     $312.00

MOUSE ANTI-HUMAN CD59 ANTIGEN

PRODUCT CODE : RDI- CBL 467   $438.00/vial 200ug

Also available:FITC labeled cat#RDI-CBL467FT   $375.00/100 Tests

                       Biotin labelled cat#RDI-CBL467BT $438.00/100 tests
                       R-PE/CY5       caT#RDI-CBL467PC5  $500.00/100T

CLONE: MEM 43

ISOTYPE: IgG2a

SOURCE: Mouse ascitic fluid.

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: A Pl-linked glycoprotein, molecular weight 18-20kDa, found in all types of leucocytes including platelets. Antigen distribution is to be found across many haemopoietic and non- haemopoietic cells.


APPLICATIONS:

* Identification of CD59 + cells by immunofluorescence and immunoperoxidase methods on cryostat sections.

* Identification of CD59 + cells in formalin fixed paraffin wax embedded sections.

* Diagnosis of paroxysmal nocturnal haemoglobinurea type II & III cells


REFERENCES:

Leucocyte Typing III, Oxford University Press. (1987).

Leucocyte Typing IV, Oxford University Press. (1989).

Horejsi, V. et al. Fol. Biol. (Prague) 34, 23 - 24 (1988).

Stefanova, I. et al. Mol. Immunol. 26, 153 - 61 (1989).


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200ug in 2ml (200T) phosphate buffered saline solution w/1mg/ml BSA & 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre.. DO NOT FREEZE CONJUGATED ANTIBODIES. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in bulk 1mg sizes with or without BSA or azide. Quotations on request (pricing starts under $1188.00/mg for purified material).

MOUSE MONOCLONAL ANTIBODY TO:  HUMAN CD59

PRODUCT CODE:RDI- CBL 547

Also available:FITC labeled   cat#RDI-CBL547FT   $438.00/100 Tests

                          PE labeled      cat#RDI-CBL547PE   $469.00/100 tests

                       Biotin labelled  cat#RDI-CBL547BT   $438.00/100 tests
                       R-PE/CY5         caT#RDI-CBL547PC5  $500.00/100T

CLONE  BRA-10G

ISOTYPE  IgG2b

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY  The antibody reacts with a 19kDa glycosylphosphatidylinositol (GPI) linked glycoprotein expressed on haematopoietic and non-haematopoietic cells.

APPLICATIONS

*Identification of CD59+ cells in formalin fixed paraffin wax embedded sections

*Identification of CD59+ cells by immunofluorescence and immunoperoxidase methods on cryostat sections

*Diagnosis of paroxysmal nocturnal haemoglobinurea type II & III cells

REFERENCES  Leucocyte Typing V, Oxford University Press (1995)

The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

DATA SHEET: mouse anti-human CD59 clone P282

Catalog#: RDI-CD59-p282   $312.00/vial    

       biotin labeled cat#RDI-CD59-p282-BT $438.00/100 T vial   Bulk on request


Package Size: 100ug purified in 0.2ml (0.PBS pH7.2 with 0.09% sodium   azide sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits- avoid contact with acids.)


Prep: purified by Protein G from TCS


Species: mouse IgG2ak


CLONE: p282 (H19)


Workshop Code: Workshop V, V S006


Activity: -not found to be inhibiting indicating a different epitope is seen than CD59 clone MEM43


Application: -Indirect immunofluorescence cell surface staining. Titer for each application

References:
  1) Schlossman, S. et al, ed.s 1995, Leucocyte Typing V, White Cell Differentiation Antigens, Oxford University Press, NY

  2) Eur J. Immunol. 22:2943

  3) Cellular Immunology 126:176

  4) Immunol RES 12:258


Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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