rev:October 6, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD57 Antibodies

Mouse anti-human CD57 antibodies

-3 clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-M2204clb                 NC1                            --            mIgM        purified     $375.00

RDI-CBL519                   NK1                            --            mIgM        purified     $438.00

RDI-CBL526                    TB01                           V           mIgM        purified     $375.00

Code: CLB-CD57

CAT#: RDI-M2204clb  $375.00/vial

Lot Number:

Tests/vial: 200

Specificity:

Cell type: NK-cells, subset T lymphocytes

Ag.MW.: 110 kD.

Reactivity: The monoclonal antibody is directed against the CD57-antigen, which is expressed on NK-cells and subset T lymphocytes. The monoclonal antibody does not react with human B lymphocytes, monocytes and thrombocytes.

Application: Identification of CD57 positive cells in tissue.

Enumberation of CD57 positive cells in peripheral blood.

Methods: Indirect staining on cryostat sections. Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: NC1

This clone has been derived from hybridization of SP2/0 Ag 1.4 cells with spleen cells of a BALB/c mouse immunized with cells from quail ciliary ganglia.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgM.

Source: Mouse ascites fluid.

Purification: Ammoniumsulphate precipitation and ion exchange chromatography.

Packing: Each vial contains 1 ml with approximately 200ug/ml monoclonal antibody and 1 mg BSA in PBS.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18Deg C. Repeated thawing and freezing may reduce the antibody activity and should be avoided.

FOR IN VITRO USE ONLY

PRODUCT CODE RDI-CBL519      $438.00/vial 200ug

CLONE: NK-1

ISOTYPE: IgM

SOURCE: mouse ascities

PURIFICATION gel filtration

SPECIFICITY: This antibody reacts with a carbohydrate antigen present on a subset of peripheral blood mononuclear cells involved in the Natural Killer activity, which is not MHC restricted. The antigen is not present on B cells, monocytes, red blood cells or platelets but is expressed on cells in neuroectodermal tissue. It reacts with 20% of normal human peripheral blood mononuclear cells.

APPLICATIONS:

*Flow cytometric studies

*formalin fixed paraffin wax embedded sections

*frozen sections

*analysis of NK cell mediated cytotoxicity

*Studies of functional properties of NK cell and T cell subsets

*Studies of neural tissue glycoproteins

*Studies of prostatic antigens

References:

Abo, T. and Balch, C.M. J. Immunology 127, 1024 (1981)

McGarry, R.C. Helfand, S.L. Quartes, R.H. and Roder, J.C. Nature 306:376 (1983)

Lanier, L.L. Le, A.M. Phillips, J.H. Wamer, N.L. and Babcock, J.F. J. Immunology 131:1789 (1983)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in in phosphate buffred saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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