rev:  May 23, 2005

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD55 Antibodies

Mouse anti-human CD55 antibodies

-2 clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CD55-C216          Bric216                        --                mIgG       purified      $500.00

RDI-M2156                  143-30                           IV              mIgG1     purified      $438.00

RDI-M2192clb             143-30                         "                 "              FITC        $438.00

RDI-CBL512                 143-30                         IV             mIgG1      purified      $375.00 

RDI-CBL512PE               " "                              "                   "            PE            $469.00

RDI-CBL512BT               " "                              "                   "            Biotin        $375.00

RDI-CBL512PC5             ""                               "                   "           RPE-CY5  $500.00/100T

You might be interested to know that about a dual labelled protocol for flow cytometry studies of paroxysmal nocturnal haemoglobinurea uses CD59-FITC and CD55PE (our clones MEM43 & 143-30). The authors indicated that the low prevalence of the CD55 required the PE label to be used with that antibody.

The published reference of interest on the CD59FITC/CD55PE is:  Practical Haematology (1994) Chapter 15 by Luzzatto & Hillman "Laboratory methods used in the investigation of paraxysomal nocturnal haemoglobinuria".

Mouse anti-Human-CD55

PRODUCT CODE RDI-CBL512     $375.00/vial 200ug

Also available: FITC labeled cat#RDI-CBL512FT     $375.00/100 test vial

                           PE labeled cat#RDI-CBL512PE    $469.00/100 test vial

CLONE: 143-30

ISOTYPE: IgG1

SOURCE: Tissue culture supernatant

Antigen: PHA activated PBMC

Fusion Partner: NS1 myeloma cell line

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: The CD55 antigen is the complement regulatory protein decay accelerating factor (DAF). It is a 70 kDa glycoportein (in erythrocytes) anchored in the membrane by a glycosylphosphatidyinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans and also one N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF has a wide distribution on cells in non- haemopoietic tissues, particular epithelium and endothelium and is specifically found at the foetal-maternal interface in placenta. Solubule forms of DAF are found for example in plasma, saliva, and urine etc.

APPLICATIONS:

* CD55 has reduced expression on cells from individuals with paroxysmal nocturnal haemoglobinuria.

-Individuals with the rare Inab phenotype have an inherited deficiencyof CD55.

REF:

-Lublin, D.M. & Atkinson, J.P. Ann. Rev. Immunol. 7, 35-38 (REVIEW) (1989)

-Daniels, G.L. Vox Sang. 56, 205-211 (Review) (1989)

-Leucocyte Typing IV. Oxford University Press pp 694-97 (1989)

-Leucocyte Typing V, Oxford University Press (in press)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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