rev:    January 29, 2004

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD54 Antibodies


Mouse anti-human CD54  antibodies

-4 clones  


CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CBL450FT                 " "                             --                 "           FITC            $375.00 

RDI-CBL450BT                 " "                              --                "           Biotin          $375.00   

RDI-CBL450PC5              ""                              --               "           R-PE/CY5    $469.00

RDI-CD54abm-A6            8.4A6                         --              mIgG1     purified        $312.00 (domain 2)

RDI-CD54abm-A6 BT      8.4A6                         --              mIgG1     biotin           $375.00 (domain 2)

RDI-CD54abm-A6FT        8.4A6                         --              mIgG1     Fitc              $375.00 (domain 2)

RDI-CD54abm-A6PE        8.4A6                         --              mIgG1     PE                $500.00 (domain 2)

RDI-CBL508                     6.5B5                         --               mIgG1   purified         $375.00

SIGNIFICANT BULK DISCOUNTS AVAILABLE!

RDI-CD54-HA58              HA58                        ----         mIgG1      purified     $281.00  


MOUSE ANTI-HUMAN CD54 ANTIGEN (ICAM-1)


PRODUCT CODE :RDI-CBL450  $375.00/vial 200ug

also available FITC labeled, cat#RDI-CBL450FT    $375.00/100T

                        PE  labeled       #RDI-CBL450PE    $469.0//100 tests

                    Biotin labeled        #RDI-CBL450BT    $375.00/100 tests

                    R-PE/CY5             #RDI-CBL450PC5   $469.00/100T


CLONE: 15.2

ISOTYPE: IgG1

SOURCE: tissue culture supernatant

PURIFICATION METHOD: Ion exchange chromatography.

SPECIFICITY: 90kDa molecular weight integral membrane glycoprotein with seven potential N-linked glycosylation sites, this is the principal ligand of LFA-I. The antigen is expressed on endothelial cells; many cell types upon activation including lymphocytes and fibroblasts.


APPLICATIONS:

* ICAM-1 is a lymphokine inducable molecule and may be used in studies of LFA-I mediated adhesion.


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200ug in 2ml PBS solution containing 10mM sodium azide and 1mg/ml BSA . We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


BULK:(1 mg or larger packed without BSA)

#RDI-CBL450-1X    


MOUSE MONOCLONAL ANTIBODY TO: HUMAN CD54 (ICAM-1)

PRODUCT CODE   RDI-CBL508    $375.00/vial 200ug

CLONE       6.5B5

ISOTYPE    IgG1

SOURCE     Tissue culture supernatant

PURIFICATION METHOD   Protein A affinity chromatography

SPECIFICITY:  This antibody recognizes a 90 kDa molecular weight integral membrane glycoprotein with seven potential N-linked glycosylation sites, this is the principal ligand of LFA-1. The antigen is expressed on endothelial cells and many other cell types upon activation including lymphocytes and fibroblasts.

APPLICATIONS

*ICAM-1 is a lymphokine inducable molecule and may be used in studies of LFA-1 mediated adhesion

*This antibody will capture the soluble form of ICAM-1 and this may be used in the development of ELISA assays for soluble CD 54.

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available in bulk (without BSA) ,quoted on request

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


DATA SHEET: mouse anti-human CD54 clone HA58  

Catalog#: RDI-CD54-HA58     $281.00/vial  

Bulk on request  -also available no azide cat#RDi-CD54-HAXP $938.00/0.5mg


Package Size: 100ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)


Prep: purified from tissue culture supernatant via Protein G affinity chromatography.


Species: mouse IgG1k


CLONE: HA58


Activity: Reacts with the 85-100kD integral membrane glycoprotein, also known as ICAM-1 (intracellular adhesion moleucle-1) expressed on endothelial cells and both resting (weak) and activated (moderate) lymphocytes and monocytes. CD54 is a ligand for leukocyte function antigen-1 (CD11a). CD54 antibodies are used for studies of inflammatory processes and neoplasia.


Application: -Indirect immunofluorescence cell surface staining ,

-Histochemistry on acetone fixed frozen sectionsTiter for each application


References: 1) Schlossman, S. et al, ed.s 1995, Leucocyte Typing V, White Cell Differentiation Antigens, Oxford University Press, NY.


Storage: Store at 4 DEG C.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


-All products are for in vitro research use only-
RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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