rev:October 6, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD50 Antibodies

Mouse anti-human CD50  antibodies

-2 clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-ICAM3                   CBR-IC3/1                    "            mIgG1       purified      $438.00

(please inquire for other clones and labeled products)

MOUSE ANTI-HUMAN CD50 (ICAM-3)

PRODUCT CODE RDI-CBL516     $375.00/vial 200ug

Also available: FITC labeled cat#RDI-CBL516FT     $375.00/100 test vial

                           PE labeled cat#RDI-CBL516PE     $469.00/100 test vial

CLONE: 101-D2

ISOTYPE: IgG1

SOURCE: Tissue culture supernatant

Antigen: MLR activated PBMC

Fusion Partner: NS1 myeloma cell line

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: CD50 (Icam-3) is the third counter receptor for LFA-1 and is a focal point for leucocyte interactions. The antigen molecule is a N-glycosylated monomer (110kDa) containing intra- chain disulphide bridges which is capable of transducing signals across the plasma membrane. T cell activation with mitogns, anti-CD3 antibodies or PMA results in the phosphorylation of the 5-cytoplasmic serine residues of ICAM-3.

APPLICATIONS:

* Studies of cell signal transduction, in particular the relationship between the tyrosine kinases p56lck and p59fyn and ICAM-3

-Studies of the soluble serum form of Human CD50 particularly in systemic lupus erythermatosus (SLE)

-Flow studies of CD50 distribution which is widely found on leucocytes.

-Immunoprecipitation studies of the CD50 antigen

ref: -Juan, M. et al, J. EXp. Med 179, 1747-1756 (1994)

     -Juan, M. et al, Eur. J. Immunol. 23, 1508-1515 (1993)

     -Villela, R. et al, Tissue Antigen 36, 203-210 (1990)

      -Pino-Otin, M.R. et al, J. Immunol. in press (1995)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME

Catalog#: RDI-ICAM3        $438.00/vial

Batch#

Package Size: 50 micrograms in 1ml PBS with 1% BSA and 0.01% thimerosal

Species: mouse IgG1

CLONE: CBR-IC3/1

Specificity: This antibody specifically recognizes human ICAM-3 and does not cross with ICAM-1, ICAM-2 or LFA-1. Not tested for other species.

Neutralization: significantly inhibits binding between LFA-1 and ICAM-3.

ICAM3 is a heavily glycosylated transmembrane 124kDa-molecule with 5 extra-

cellular domains. In contrast to ICAM-1 and ICAM-2 it is particularly expressed on

resting lymphocytes, monocytes, and neutrophils. Icam-3 is not found on

endothelial cells.

Together with ICAM-1 and ICAM-2, ICAM-3 acts as a ligand for LFA-1 and binds this B2-

integrin through its 2 N-terminal domains. All three Icams-molecules own distinct

features during the interaction between leukocytes and their different targets. In

this context ICAM-3 is potentially the most important ligand for LFA-1 in the initiation

of the immune response, e.g. the adhesion of resting T-lymphocytes to antigen presenting

B-cells. After immune cell activation the importance of ICAM-3 decreases because ICAM-1

is upregulated to a much greater extent.

An involvement of ICAM-3 in the T-cell mediated lysis of infected cells and the

allogenetic as well as autologous mixed lymphocyte reaction is discussed.

Application: Anti-ICAM-3 can be used to stain acetone fixed cryostat sections or cell smears. The initial dilution is 1:40 to 1:100 (optimal dilution should be determined by the individual lab for each application. -The antibody may also be used in immunofluorescence. -neutralization: significantly inhibits binding between LFA-1 and ICAM-3.

Storage: Store at 4 DEG C.

ref: J. Exp. Med, 175, 185-190

     Nature 360,481-488.

     J. Exp. Med,174,253-267.

    Vth Workshop, Oxford Press

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or

for diagnostics.

It is the responsibility of the user to comply with alllocal/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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