rev: January 7, 2004
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Mouse anti-human CD49d antibodies
CATALOG# CLONE# Workshop Host Form Price
RDI-CBL536 BU49 VI mIgG1 purified $375.00
RDI-CBL536FT " " " FITC $375.00
RDI-CBL536PE " " " PE $438.00
RDI-CD49D-BU49BT " " " Biotin $375.00/100ug
RDI-CD49D-HP21 HP2/1 V mIgG1 purified $594.00 (multi-species)
PRODUCT CODE : RDI- CBL 485 $438.00/vial 200ug
SOURCE: Tissue culture supernatent.
Immunizing Antigen: Hoon pre-B leukamia cell line
Fusion Partner: Tissue culture supernatant
PURIFICATION METHOD: Protein A affinity chromatography.
SPECIFICITY: The antibody reacts with the 150kDa VLA-a-4 chain which is associated with the CD29 molecule in the VLA-4 complex. Expressed on ALL and AML cells, T and B-cells, monocytes, thymocytes and Langerhans cells. There is no reactivity with human kidney or brain sections.
* FACS and immunoprecipitation studies of CDw49d antigen.
-This antibody will induce homotypic aggregation
-Quackenbush, E.J. and Letarte, M. J. Immunol 134, 1276-1285 (1985)
-Letarte, M. et al, Leukemia (in press) 1992
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200ug milligrams in 2ml in phosphate buffered saline with 10mM sodium azide and 1mg/ml BSA. We recommend that each laboratory determine an optimum working titre for use in its particular application. Additional care should be taken with any preservative free material to reduce bacterial contamination (optional).
STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
Also available in bulk, with or without BSA or preservative.
1 milligram =$1062.00, 2-4mg $938.00/mg. Larger sizes quoted on request order cat#RDI-CBL485-1XP (NO BSA, NO AZIDE).
Product Code: RDI-CBL536 $375.00/vial
RDI-CBL536FT $375.00/100T vial
Immunizing Antigen: Hyatt myeloma cell line.
Fusion Partner: NSO myeloma cell line.
Source: Tissue culture supernatant
Purification Methods: Protein A affinity chromatography.
Specificity: The antibody recognises the 150 kDa VLA-a-4 chain (lymphocyte homing receptor) which is associated with the CD29 molecule in the VLA-4 complex. The antigen is expressed on ALL and AML cells, T and B cells, monocytes, thymocytes and Langerhans cells. There is no r reactivity with human kidney or brain sections.
Applications: Suitable for basic studies of VLA-4 FACS and immunoprecipitation studies of CDw49d antigen.
References: Leucocyte typing VI (1997) in press.
Presentation: The monoclonal is presented at a concentration of 200 ug/2ml in phosphate buffered saline containing 10mM sodium azide and 1 mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage: For use within 1 month of purchase store at +4'C, for long term storage aliquot antibody into small volumes and store at -20'C.
-Bulk, no azide, no BSA available upon request, cat#RDI-CBL536-1XP $1062.00/1mg)
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.
cat# RDI-CD49D-HP21 $594.00
Presentation: 200ug in 1ml (1mg/ml) phosphate Buffered Saline pH7.4 with 0.09% Sodium Azide
Clone Number: HP2/1
Purified: IgG prepared by affinity chromatography on Protein G Buffer:
Immunogen: JM leukaemia line
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63 Ag8.653 myeloma cell line.
Isotype: IgG1 (Mouse)
Specificity: MAB recognises the 80kD alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells. Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.
Species Cross: Clone is reported to cross react with porcine (4), rhesus monkey (7, 9), bovine (6) and rat (8).
N.B. Antibody reactivity and working conditions may vary between species.
Applications: This product has been reported to work in the following applications. This information is derived from testing within producer laboratories, from peer-reviewed publications or from personal communications from antibody originators. Please refer to references indicated for further information
Flow Cytometry 1ug/5 x 10 E05 cells
Immunohistology - frozen (approx 10ug/ml)
-not tested for paraffin sections - paraffin
-Western Blotting: not tested
storage: This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Safety: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References: 1. Sanchez-Madrid, F. et al. (1986). VLA-3; a novel polypeptide association within the VLA molecular complex: cell distribution and biochemical characterization. Eur. J. Immunol. 16: 1343-1349.
2. Weller. P.F. et al. (1991). Human eosinophil adherence to vascular endothelium mediated by binding to vascular cell adhesion molecule 1 and endothelial leukocyte adhesion molecule 1. P.N.A.S. USA 88: 7430.
3. Mattila, P. et al. (1992). VLA-4 integrin on sarcoma cell lines recognises endothelial VCAM-1. Differential regulation of the VLA-4 avidity on various sarcoma cell lines. Int. J. Cancer 52: 918-923.
4. Leucocyte Typing Workshop V p. 1646-1648.
5. Sopper, S. et al. (1997). Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry 29: 351-362.
6. Van Vliet, S.S. et al. (1995). Species cross reactivity (human-monkey-pig-bovine) of the adhesion structure section mAB. Leucocyte Typing V. Oxford University Press p 1607-1608.
7. Schmitz, J. et al. (2001). Simian immunodefiency virus (SIV) - specific cytotoxic T lymphocytes in gastrointestinal tissues of chronically SIV-infected rhesus monkey. Blood. 98: 3757 - 3761.
8. Sanz, M. et al. (1997). Tumor necrosis factor alpha-induced eosinophil accumulation in rat skin is dependant on alpha 4 integrin/vascular cell adhesion molecule-1 adhesion pathways. Blood. 90: 4144 - 4152.
9. Kuroda, M. et al. (1999). Comparitive analysis of cytotoxic T lymphocytes in lymphnodes and peripheral blood of simian immunodeficiency virus-infected rhesus monkeys. J. Virol. 73 (2): 1573 - 1579. Storage Conditions: Store at +4oC or at -20oC if preferred.
FOR RESEARCH PURPOSES ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE
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Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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