rev:  June 7, 2005

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: . Please inquire for bulk or custom formulations (without preservative or carrier).

RDI div of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD49b Antibodies

Mouse anti-human CD49b antibodies

-2 clones   & 1 polyclonal

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-M1540clb              CLB-tromb/4, 10G11      IV          mIgG1       purified     $375.00

RDI-M1671clb                  " "                                "               "             FITC        $375.00

RDI-CBL477                 AK7                               V           mIgG1       purified      $438.00

RDI-CBL477FT               " "                                 "              "             FITC         $438.00

RDI-CBL477PE               " "                                 "             "              PE             $531.00

RDI-CD49Babr             rabbit poly

Product Specification: mouse monoclonal anti-human CD49b

PeliCluster  CD49e

cat#RDI-M1540clb

Test/vial 200


Clone CLB-tromb/4, 10G11


This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes. This antibody has been clustered to CD49b in the fifth international Workshop on Human White Cell differentiation Antigens in Boston (1993).


Isotype Mouse IgG1.


Source Ascites fluid of tumour bearing BALB/c mice.


Purification Ammoniumsulphate precipitation and ion exchange chromatography.


Packing Each vial contains 1 ml with approximately 0.2 mg/ml monoclonal antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.


Preservative Mertiolate (0.001%). or sodium azide (see batch sheet)


Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.


Major reactivity The monoclonal antibody is directed against the CD49b- antigen (GP Ia or VLA-2 alpha-chain), which can form distinct complexes with either the CD29-antigen (GP IIa or VLA beta-chain), resulting in the VLA-2 (alpha-2 beta- 1) complex, which is expressed on human platelets. The monoclonal antibody reacts with platelets, long-term cultivated T lymphocytes and activated T lymphocytes. In immunohistology the monoclonal antibody reacts with thymocytes, epithelial cells of a variety of tissues, peripheral nerves, fibroblasts, osteoclasts, glomerular mesangium and most non-haemopoietic adherent cell lines.


Molecular mass 130, 170 kDa.


Application Functional studies on cells.

Detection of human alloantibodies (anti-Bra,b) against VLA-2 (MAIPA assay).


Methods Indirect immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.


References

1. Knicki, T.J. et al., J. Biol. Chem., 263, 4516 (1988).

2. Giltay, J.C. et al., Blood, 73, 1235 (1989).

3. Staatz, W.D. et al., J. Cell. Biol. 108, 1917 (1989).


APPLICATION FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE

Reagents

- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).

   Remove aggregates by centrifugation at 1000 x g for 10 minutes.

- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).

- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.

- Microtiter plates (96 wells, V bottom) or tubes.


Procedure

1   Fixate the cells with PFA 1% and wash them with buffer.

2   Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.

3   Add 45 µl of cell suspension to the microtiter wells or tubes.

4   Add 5 µl monoclonal antibody to the microtiter wells or tubes and mix gently.

5   Incubate for 30 minutes at 2-8 C.

6   Add 100 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

7   Aspirate the supernatant from the cell pellet and resuspend the cells.

8   Add 200 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

9   Aspirate the supernatant from the cell pellet and resuspend the cells.

10  Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human                                 immunoglobulins,       diluted 1:80.

11  Incubate for 30 minutes at 2-8 C.

12  Repeat step 6-9.

13  Add 200 µl buffer.

14  The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.


NOTE: Care should be taken when drawing blood to avoid activation of platelets.


Platelets activation markers are weakly expressed on platelets prepared from directly fixed normal whole blood.

The expression is maximal on thrombin (1 U/ml) stimulated washed platelets.


FOR IN VITRO RESEARCH USE ONLY

Product Specification: mouse nonoclonal CD49b,conjugated to Fluorescein


PeliCluster  CD49b F


cat#RDI-M1671clb


Test/vial 100


Clone CLB-tromb/4, 10G11


This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes. This antibody has been clustered to CD49b in the fifth international Workshop on Human White Cell differentiation Antigens in Boston (1993).


Isotype Mouse IgG1.


Source Ascites fluid of tumour bearing BALB/c mice.


Purification Ammoniumsulphate precipitation and ion exchange chromatography.


Conjugation Cojugated with fluorescein iso thiocyanate isomer 1 (FITC).

Molecular F/P ratio between 6.0 - 10.0


Packing Each vial contains 1 ml FITC conjugated monoclonal antibody and 10 mg BSA in PBS.


Preservative Mertiolate (0.001%) or sodium azide


Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.


Major reactivity The monoclonal antibody is directed against the CD49b- antigen (GP Ia or VLA-2 alpha-chain), which can form distinct complexes with either the CD29-antigen (GP IIa or VLA beta-chain), resulting in the VLA-2 (alpha-2 beta- 1) complex, which is expressed on human platelets. The monoclonal antibody reacts with platelets, long-term cultivated T lymphocytes and activated T lymphocytes. In immunohistology the monoclonal antibody reacts with thymocytes, epithelial cells of a variety of tissues, peripheral nerves, fibroblasts, osteoclasts, glomerular mesangium and most non-haemopoietic adherent cell lines.


Molecular mass 130, 170 kDa.


Application Detection of human alloantibodies (anti-Bra,b) against VLA-2 (MAIPA assay).


Methods Direct immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.


References

1. Knicki, T.J. et al., J. Biol. Chem., 263, 4516 (1988).

2. Giltay, J.C. et al., Blood, 73, 1235 (1989).

3. Staatz, W.D. et al., J. Cell. Biol. 108, 1917 (1989).


FOR IN VITRO RESEARCH USE ONLY

Mouse ANTI-Human CDw49b ANTIGEN(COLLAGEN RECEPTOR)


PRODUCT CODE :RDI-CBL477     $438.00/vial 200ug

Also available FITC labeled cat#RDI-CBL477-FT   $438.00/100 tests

                          PE labeled cat#RDI-CBL477-PE   $531.00/100 tests


CLONE: AK7


ISOTYPE: IgG1


SOURCE: Mouse ascitic fluid


PURIFICATION METHOD: Ammonium sulphate precipitation folloed by ion exchange chromatography.


SPECIFICITY: Human platelet antigen (Mr 170kD). The antigen is found on platelets, endothelial cells and approximately 50% of monocytes together with activated T cells. The gpla antigen is non- covalently associated with the 130kD VLA ß-chain (CD29) in the VLA-2 complex.


APPLICATIONS:

*This antibody reacts with the platelet gp Ia molecule (a-chain of the adhesion molecule VLA-2), the platelet collagen receptor.

* Detection of human alloantibodies against VLA-2.


REFERENCES:

Favaloro, E.J. et al. Bs. J. Haem. 74, 385 - 94 (1990)

Mazurov, A.V. et al. Thromb. Haemost. 66, 494 - 99 (1991)


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 2 vial of 100ug/1ml each in phosphate buffered saline solution containing 10mM sodium azide and 1mg/ml BSA. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

DATA SHEET: rabbit anti-INTEGRIN Alpha 2 (CD49b)

Catalog#: RDI-CD49Babr   $469.00

Package Size: 100ul ,liquid neat sera with 0.05% sodium azide

Species: rabbit polyclonal

Immunogen: synthetic peptide derived from the c terminal cytoplasmic domain of human alpha2 integrin subunit

(KYEKMTKNPDEODETTELSS)

Reactivity: reacts with CD49B (alpha 2 integrin subunit in human. Sees a cytoplasmic/intracellular epitope. No cross observed with alpha-1, alpha-3, alpha4, alpha5, alpha6 or alpha V subunits

species: Human, rat, mouse, hamster, chicken

Uses: -immunoblotting(non reducing conditions may be better) 1:1000

-immunoprecipitation: 5ul per 5 million cells

-acetone fixed frozen tissue

Titers must be optimized for each application

ref: Deroanne, CF et al, Exper Cell Res (1996) 224:215-223

Storage: Store at -20 DEG C in undiluted aliquots. For extensive dilutions, add protein containing or stabilizing medium. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. No for use in or on humans or animals or for diagnostics.

Note: Upon receipt, tighten cap, and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in vial (during shipping, some antibody can become lodged inside the cap and on sides of vial

RDI Div of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049  USA

phone (978) 371-6446

fax     (978) 371-2266

ordering terms

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