rev: May 2, 2007
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CD CLUSTERED
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD46
Antibodies
Mouse anti-human CD46 antibodies
-3 clones
CATALOG# CLONE# Workshop Host Form Price
RDI-CBL488 J4-48 IV mIgG1 purified $375.00
RDI-CD46E43X E4.3 -- mIgG2a purified $312.00
RDI-CD46abm-4PE E4.3 -- mIgG2a PE $500.00
RDI-CD46abm-4FT E4.3 -- mIgG2a FITC $375.00
(Membrane Cofactor Protein)
PRODUCT CODE :RDI-CBL488 $375.00/vial of 200ug
CLONE J4-48
ISOTYPE IgG1
SOURCE Mouse ascitic fluid
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY
This antibody is specific for membrane cofactor protein (CD46) (MCP), which is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells. A soluble MCP is found in semen and human tear film.
APPLICATIONS
* Study of MCP in its binding to C3b or C4b
* MCP may mediate in the protection of autologous tissues and trophoblasts from complement mediated damage
REFERENCES
Leucocyte Typing IV, Oxford University Press (1989)
Liszewski, M.K. et al. Ann. Rev. Immunol. 10, 431-455 (1991)
PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml
in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine
serum albumin. We recommend that each laboratory determine an optimum working
titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage
aliquot antibody into small volumes and store at -20oC.
For Research Use Only. Not supplied for use in diagnostic or therapeutic
Also available in bulk, quotations on request.
PRODUCT CODE RDI-CBL515 $375.00/vial 200ug
Also available: FITC labeled cat#RDI-CBL515FT $375.00/100 test vial
PE labeled cat#RDI-CBL515PE $469.00/100
test vial
Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME
CLONE 122-2
ISOTYPE IgG1
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY
This antibody is specific for membrane cofactor protein (CD46), which is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells. A soluble MCP is found in semen and human tear film
APPLICATIONS
* Study of MCP in its binding to C3b or C4b
* MCP may mediate in the protection of autologous tissues and trophoblasts from complement mediated damage
REFERENCES
Leucocyte Typing IV, Oxford University Press (1989)
Liszewski, M. K. et al. Ann. Rev. Immunol. 9, 431-455 (1991)
Hara, T. et al. Clin. Exp. Immunol. 89, 490-94 (1992)
PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
Catalog#: RDI-CD46E43X $312.00/100ug Bulk quotes on request
PE conjugated RDI-CD46abm-4PE $500.00/120T
FITC conjugated: RDI-CD46abm-4FT $375.00/120T
Package Size: 100ug in 0.1ml (1mg/ml) 50mM sodium phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5mg/ml gentamicin sulfate.
Prep: purified by Protein A from TCS. Purity >95% by SDS-Page.
CLONE: 169-1-E4.3
Isotype: Mouse IgG2ak
Immunogen: Human PBL and EBV transformed LCL
Epitope: sees the SCR1 epitope of human CD46 -does not block MCP function
Application: -Indirect fluorescence
-histochemistry on frozen sections (not tested on paraffin)
-immunoprecipitation
-western blotting (non reducing conditions)
-immunoassay
-titers must be determined for each application
Storage: Store at 4 Deg C
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
References: -Sparrow et al (1983). Hum. Immunol. 7: 1-15
-Sparrow et al (1985). Hum. Immunol 13:83-93
-Purcell et al. (1989). Cell Biol. 67: 279-289
-Che et al (1991). Clin Exp. Immunol. 83:257- 261
-Loveland et al. (1993). Transplant. Immunol. 1:101-108.
-Kurita, M.Y. et al, 1995: Immunol. Lett 48:91
PERFORMANCE: Briefly, 500K cultured human HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD46 antibody at 5 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.20 log10 fluorescent units when compared to a Mouse IgG2a (cat#RDI-MIGG2AX $188.00/100ug) negative control at a similar concentration.
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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