rev:  May 24, 2005

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD45RO  Antibodies

Mouse anti-human CD45ro  antibodies

-1 clone 

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

Mouse ANTI-Human CD 45RO (ANTI-T LYMPHOCYTES)

PRODUCT CODE :RDI-CBL133     $375.00/vial 200ug

also available: FITC labeled cat#RDI-CBL13FT        $375.00/100 tests

                          PE labeled cat#RDI-CBL133PE      $469.00/100

CLONE: UCHL1

ISOTYPE: IgG2a

SOURCE: Mouse ascitic fluid

PURIFICATION METHOD: Protein A affinity chromatography

SPECIFICITY: 180kD MW Leucocyte surface antigen present on a subpopulation of resting T lymphocytes, monocytes and granulocytes. The antigen represents the low MW component of the Leucocyte Common Antigen (LCA) and is demonstrable on 48%of peripheral blood T lymphocytes, 37% of CD4+ lymphocytes, 19% of CD8+ lymphocytes, 80% of thymocytes and the majority of T cell malignancies. Monocytes and granulocytes have surface expression of the antigen and tissue macrophages exhibit cytoplasmic expression.

The antigen survives formalin fixation and conventional tissue processing to wax sections.

APPLICATIONS:

* Identification of helper inducer and memory cytotoxic T lymphocytes by indirect immunofluorescence staining.

* *Identification of normal and malignant T lymphocytes in formalin fixed, wax embedded tissue sections (using high temp release).

* Immunoprecipitation of the 180kD component of the LCA

* Isolation or removal of helper inducer or memory cytotoxic T lymphocytes by FACS or panning techniques

REFERENCES:

Leucocyte Typing III, Oxford University Press 1987, pp225-7, 800

Smith S.H., et al Immunology 58, 63 (1986)

Norton A.J., et al J. Clin. Pathol. 39, 399 (1986)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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