rev: 5/16/2008
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD45RO
Mouse anti-human CD45ro antibodies
-1 clone
CATALOG# CLONE# Workshop Host Form Price
Mouse ANTI-Human CD 45RO (ANTI-T LYMPHOCYTES)
PRODUCT CODE :RDI-CBL133 $412.50/vial
200ug
also available: FITC labeled cat#RDI-CBL13FT $375.00/100 tests
PE labeled cat#RDI-CBL133PE $469.00/100
CLONE: UCHL1
ISOTYPE: IgG2a
SOURCE: Mouse ascitic fluid
PURIFICATION METHOD: Protein A affinity chromatography
SPECIFICITY: 180kD MW Leucocyte surface antigen
present on a subpopulation of resting T lymphocytes, monocytes
and granulocytes. The antigen represents the low MW component of the Leucocyte Common Antigen (LCA) and is demonstrable on 48%of
peripheral blood T lymphocytes, 37% of CD4+ lymphocytes, 19% of CD8+
lymphocytes, 80% of thymocytes and the majority of T
cell malignancies. Monocytes and granulocytes have
surface expression of the antigen and tissue macrophages exhibit cytoplasmic expression.
The antigen survives formalin fixation and conventional tissue processing to
wax sections.
APPLICATIONS:
* Identification of helper inducer and memory cytotoxic T lymphocytes by indirect immunofluorescence staining.
* *Identification of normal and malignant T lymphocytes in formalin fixed, wax embedded tissue sections (using high temp release).
* Immunoprecipitation of the 180kD component of the LCA
* Isolation or removal of helper inducer or memory cytotoxic T lymphocytes by FACS or panning techniques
REFERENCES:
Leucocyte Typing III, Oxford University Press 1987, pp225-7, 800
Smith S.H., et al Immunology 58, 63 (1986)
Norton A.J., et al J. Clin. Pathol. 39, 399 (1986)
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration
of not less than 200µg/2ml in phosphate buffered saline containing 10mM
sodium azide and 1mg/ml of bovine serum albumin. We
recommend that each laboratory determine an optimum working titre
for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods
at 4oC. However repeated warming to room temperature and re-cooling may result
in a loss of activity and hence effective working titre.
It is recommended that monoclonal antibodies are aliquotted
upon receipt and then stored at -20C. After thawing they should then be used as
a stock solution for up to 60 days. Dilute working solutions are best prepared
on the day of use.
For research use only. Not supplied for use in human
diagnostic or therapeutic procedures
RDI Div of Fitzgerald Industries Intl
(Research Diagnostics Inc)
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
email: sales@researchd.com
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