rev: 5/16/2008
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD41a Antibodies
Mouse anti-human CD41a antibodies
-3 clones (new clone)
CATALOG# CLONE# Workshop Host Form Price
RDI-M1538clb CLB-tromb/7(48),6C9 IV mIgG1 purified $375.00
RDI-M1674clb " " " " FITC $375.00
RDI-CBL130 DD4.1 VI mIgG1 purified $375.00
RDI-CBL130FT " " -- " FITC $375.00
RDI-CBL130PE " " -- " PE $469.00
RDI-CBL130BT "" -- " Biotin $375.00
RDI-CD41HIP8 HIP8 IV mIgG1 purified $312.00
RDI-CD41a-2C 96.2C1 --- mIgG1 purified $312.00
RDI-CD41a-2CBT " -- "" Biotin $412.50/100ug
RDI-CD41A-2CPE " -- "" PE $531.00/120T
Product Specification: mouse monoclonal anti-human CD41A
PeliCluster CD41
Cat#RDI- M1538clb
Test/vial 200
Clone CLB-tromb/7, 6C9
This clone has been derived from hybridization of SP2/0 cells with spleen cells
of a BALB/c mouse immunized with human platelets. This antibody has been
clustered to CD41 in one of the international Workshop on Human White Cell
differentiation Antigens.
Isotype Mouse IgG1.
Source Ascites fluid of tumour bearing BALB/c mice.
Purification Ammoniumsulphate precipitation and ion exchange chromatography.
Packing Each vial contains 1 ml with approximately 0.2 mg/ml monoclonal
antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.
Preservative Mertiolate (0.001%).
Storage and stability Monoclonal antibodies should be stored in the dark at
2-8°C. The reagent is stable until the expiry date stated on the vial
label.
Major reactivity The monoclonal antibody recognizes the intact platelet
gpIIb/IIIa complex, i.e. it does not bind to dissociated gpIIb or gpIIIa. The
antibody does not bind to platelets from patients with Glanzmann Thrombasthenia
and does not recognize the vitronectin receptor, which contains gpIIa as a beta
subunit (cf. CD61). The monoclonal antibody activates human thrombocytes and
induces thrombocyte aggregation in the absence of thrombocyte inhibitors such
as EDTA.
Molecular mass 25 kDa.
Application The monoclonal antibody is a useful marker for megakaryoblasts and
megakaryoblastic leukaemias.
Methods Indirect immunofluorescence staining with analysis by flowcytometry or
fluorescence microscopy.
References 1 Modderman, P.W. et al., Thrombosis and Haemostasis, 60, 68 (1988).
APPLICATION FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE
Reagents
- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).
Remove aggregates by centrifugation at 1000 x g for 10 minutes.
- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).
- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.
- Microtiter plates (96 wells, V bottom) or tubes.
Procedure
1 Fixate the cells with PFA 1% and wash them with buffer.
2 Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.
3 Add 45 µl of cell suspension to the microtiter wells or tubes.
4 Add 5 µl monoclonal antibody to the microtiter wells or tubes and mix gently.
5 Incubate for 30 minutes at 2-8 C.
6 Add 100 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
7 Aspirate the supernatant from the cell pellet and resuspend the cells.
8 Add 200 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
9 Aspirate the supernatant from the cell pellet and resuspend the cells.
10 Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human immunoglobulins, diluted 1:80.
11 Incubate for 30 minutes at 2-8 C.
12 Repeat step 6-9.
13 Add 200 µl buffer.
14 The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.
NOTE: Care should be taken when drawing blood to avoid activation of
platelets.Platelets activation markers are weakly expressed on platelets
prepared from directly fixed normal whole blood. The expression is maximal on
thrombin (1 U/ml) stimulated washed platelets.
FOR IN VITRO RESEARCH USE ONLY
Product Specification: mouse monoclonal CD41,conjugated to Fluorescein
PeliCluster CD41 F
CAT#RDI- M1674clb
Test/vial 100
Clone CLB-tromb/7, 6C9
This clone has been derived from hybridization of SP2/0 cells with spleen cells
of a BALB/c mouse immunized with human platelets. This antibody has been
clustered to CD41 in one of the international Workshop on Human White Cell
differentiation Antigens.
Isotype Mouse IgG1.
Source Ascites fluid of tumour bearing BALB/c mice.
Purification Ammoniumsulphate precipitation and ion exchange chromatography.
Conjugation Conjugated with fluorescein iso thiocyanate isomer 1 (FITC).
Molecular F/P ratio between 5.5 - 9.5.
Packing Each vial contains 1 ml FITC conjugated monoclonal antibody 10 mg BSA
in PBS.
Preservative Mertiolate (0.001%).
Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.
Major reactivity The monoclonal antibody recognizes the intact platelet gpIIb/IIIa complex, i.e. it does not bind to dissociated gpIIb or gpIIIa. The antibody does not bind to platelets from patients with Glanzmann Thrombasthenia and does not recognize the vitronectin receptor, which contains gpIIa as a beta subunit (cf. CD61). The monoclonal antibody activates human thrombocytes and induces thrombocyte aggregation in the absence of thrombocyte inhibitors such as EDTA.
Molecular mass 25 kDa.
Application The monoclonal antibody is a useful marker for megakaryoblasts and megakaryoblastic leukaemias.
Methods Direct immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.
References 1 Modderman, P.W. et al., Thrombosis and Haemostasis, 60, 68 (1988).
FOR IN VITRO RESEARCH USE ONLY
MOUSE ANTI-HUMAN CD41a ANTIGEN (NEW specs in press)
PRODUCT CODE :RDI-CBL130 $375.00/vial
also available:FITC labeled cat#RDI-CBL130FT $375.00/100 tests
PE labeled #RDI-CBL130PE $469.00/100 tests
Biotin labeled #RDI-CBL130BT $375.00/100 tests
CLONE: DD4.1
ISOTYPE: IgG1
Immunizing Antigen: Medulloblastoma tissue
Fusion Partner: NS1 myeloma cell line
SOURCE:tissue culture supernatant
PURIFICATION METHOD: Protein A affinity chromatography
SPECIFICITY: The antibody recognises the intact platelet gpIIb/ IIIa complex, it does not bind to dissociated gpIIb or gpIIIa only to gpIIb in the intact complex. This is found on platelets, megakaryocytes and monocytes. Acute megakaryoblastic l leukaemia cells are positive for the gpIIb/IIIa antigen whereas common myeloid leukaemias are negative.
Applications: -Identification of the a-integrin chain of gpIIb/IIa expression
on platelets, megakaryocytes and monocytes by indirect immunofluorescence
staining.
-Acute leukaemia phenotyping.
-Studies of Glanzmann's thrombasthenia where gpIIb/IIIa is absent or significantly reduced.
-Ligand binding occurs in part via an RGD sequence which is known to be of importance for certain other adhesion interactions with extracellular proteins.
References: Kemshead, J. Brit, J. Haem, 57, 621-24 (1984)
Kieffer, N. & Phillips, D.R. Ann. Rev. Cell Biol. 6, 334-57 (1990).
Leucocyte Typing VI, Oxford University Press (in press).
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration
of not less than 200µg/2ml in phosphate buffered saline containing 10mM
sodium azide and 1mg/ml of bovine serum albumin. We recommend that each
laboratory determine an optimum working titre for use in its particular
application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods
at 4oC. However repeated warming to room temperature and re-cooling may result
in a loss of activity and hence effective working titre. It is recommended that
monoclonal antibodies are aliquotted upon receipt and then stored at -20C.
After thawing they should then be used as a stock solution for up to 60 days.
Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic
procedures
DATA SHEET: mouse anti-human CD 41A clone HIP8
Catalog#: RDI-CD41HIP8 $312.00/100ug
Package Size: 100ug in 0.2ml, 0.5mg/ml) buffer + 0.09% NaN3)
Species: mouse IgG1
Purification: purified from tissue culture supernatant by Protein G affinity
chromatography
CLONE: HIP8
Workshop: IV, #P38
Activity: Reacts with calcium dependent complex of CD41/CD61 (GPIIb/IIIa)
expressed on platelets and megakaryocytes. CD41/CD61 complex is the receptor of
fibrinogen, fibronectin and von Willebrand factor, and mediates platelet
adhesion and aggregation. Clone HIP8 antibody completely inhibits platelet
aggregation and ATP secretion induced by ADP, thrombin, and collagen. This
antibody is useful in the morphological and physiological studies of platelets
and megakaryocytes.
Application: -Indirect fluorescence
-adhesion (after removing azide)
-titers must be determined for each application
Storage: Store at 4 Deg C .
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics.
References: -Leucocyte Typing V, 1995
For In Vitro Research Use Only
-also available FITC labeled cat#RDI-CD41HIP8-FT $438.00/100T
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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