rev:   May 28, 2002

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD35 Antibodies

Mouse anti-human CD35 antibodies

-1 clone  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CBL157                       E11                          IV           mIgG1       purified     $375.00

RDI-CBL157FT                    "                              "                "             FITC        $375.00

RDI-CBL157PE                    "                              "                "             PE            $469.00

RDI-CD35abm-24               KuN241                   --             mIgG1      purified    $406.00  (paraffin sections)

PRODUCT CODE :RDI-CBL 157     $375.00/vial purified 200ug

also available FITC labeled: cat#RDI-CBL157FT      $375.00/100 tests

                         PE labeled: cat#RDI-CBL157PE      $469.00/100 tests

CLONE               E11

ISOTYPE           IgG1

SOURCE             Tissue culture supernatant

PURIFICATION METHOD Protein A affinity chromatography

SPECIFICITY The CD35 antigen, CR1, is a complement component receptor with specificity for C3b, C4b and iC3b. The antigen is a 220 kDa molecule with polymorphism in a low percentage of individuals that results in molecular forms of 250 and 220/250 kDa. The CD35 antigen is expressed by diverse cell types including erythrocytes, B lymphocytes, monocytes, granulocytes, and kidney podocytes.

APPLICATIONS *Studies of CD35 expression on viable cells by indirect immunofluorescence staining

Studies of CD35 expression by cells in frozen tissue sections by indirect immunofluorescence staining

*Studies of CD35 expression in wax sections of formal/acetic fixed tissue; staining may be possible in formalin fixed tissue but so far this approach has proved unreliable

-western blot (using HeLa cells as positive control bands approx 220 and 20kda)

REFERENCES Hogg. N. Eur. J. Immunol. 14, 236 (1984)Leucocyte Typing III, Oxford University Press (1987)Tay, S. K. et al. Br. J. Obstet & Gynaecol. 94 (II) 1094-7 (1987)Allen, C. & Hogg, N. J. Natl Cancer Inst. 78 (3) 465-70 (1987)

LOT SPECIFIC DATA

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures

cat# RDI-CD35abm-24      $406.00/100ug

       RDI-CD35abm-24X    $562.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: Cells from an acute lymphocytic leukemia (ALL) patient.

Isotype: mIgG1

Clone: KuN241

Reactivity: human (not rat) (others not tested)

Uses: -histochemistry (frozen and paraffin sections) approx 2-4ug/ml for 60 minutes RT

        -for paraffin sections, requires boiling tissue sections in 1mM EDTA buffer pH8.0 for 10-20 minutes followed by cooling at RT for 20 minutes. -approx ab concentration 2-10ug/ml for 1 hr RT

          -flow cytometry

-able to induce Ca 2+ signals in PMNs and monocytes by virtue of its binding to CD32 through the Fc part and CR1 binding   through the Fab domain (order azide free cat#RDI-CD35abm-24XP $625.00/200ug in 0.2ml no BSA/No Azide

Reference: 1. Mathew JM; et al. Hybridoma, 1995, 14:29-35.

Positive Control: tonsil

Localization: cell membrane

Background: Complement receptor 1 (CR1, CD35)is a cell membrane-bound, monomeric glycoprotein that is found on numerous cell types of primates including erythrocytes, lymphocytes, and phagocytes The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules.

Storage: 2-8 deg c

For Research Use Only

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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