rev:  October 3, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD3 Antibodies


Mouse anti-human CD3 antibodies

-5+ clones   CLB-T3/2,16A9 (purified & FITC)

                  UCHT1 (purified, FITC ,PE & R-PE/CY5)

                  Cris 7    (purified, FITC , PE 7 R-PE/CY5)

                   Mem92 (purified)

                  CLB-T3/4.E, 1XE  (ascites, IgE subclass antibody especially prepared and tested                                                                                               for  T cell stimulation)

                  CD3 polyclonals/monoclonals (for blotting and  paraffin sections-see below, rat/mouse/human)

                   Click here for a new CD3 monoclonal for paraffin sections (human)


Catalog#                  CLONE                   Workshop      Isotype     Form         Price

RDI-M1306clb        CLB-T3/2,16A9           IV              mIgG2a    purified    $375.00

RDI-M1466clb             " "                             "                   "           FITC        $375.00

RDI-CBL150           UCHT1                        III              mIgG1      purified    $375.00

RDI-CBL150FT           " "                             "                 "             FITC       $375.00

RDI-CBL150PE           " "                             "                 "             PE           $469.00

RDI-CBL150BT           " "                             "                 "             BIOTIN   $375.00

RDI-CBL150PC5        "  "                             "                 "            R-PE-CY5 $469.00

RDI-CBL126           Cris 7                            II              mIgG2a    purified     $375.00

RDI-CBL126PC5       "   "                              "                 "          R-PE-CY5  $469.00

RDI-CBL126BT          " "                               "                 "           BIOTIN    $375.00

RDI-CD3-SP34         SP34                            V                mIgG3   purified     $344.00

                            (also sees baboon/rhesus/cynolmolgus)

RDI-CD3-SP34FT      "                                   "                "           FITC        $344.00/50tests

RDI-SP3-SP34XP        "                                 --                "           no azide  $812.00/0.5mg


PeliCluster   CD3

cat#RDI-M1306clb   $375.00/vial


Test/vial 200


Clone CLB-T3/2, 16A9

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes. This antibody has been clustered to CD3 in one of the international Workshop on Human White Cell differentiation Antigens

Isotype The monoclonal antibody is of the mouse subclass IgG2a.

Source Ascites fluid of tumour bearing BALB/c mice.


Purification Ammoniumsulphate precipitation and ion exchange chromatography


Packing Each vial contains 1 ml with approximately 0.1 mg/ml monoclonal antibody and

10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.


Preservative Mertiolate (0.001%).


Storage and stability Monoclonal antibodies should be stored at 2- 8°C. The reagent is stable until the expiry date stated on the vial label.


Major reactivity The monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.


Molecular mass 20, 25, 28 kD.

Application Monitoring of T-cell numbers in peripheral blood. Characterization of leukaemias and lymphomas. Identification of T-cells in tissue.

Methods Indirect immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.

FOR IN VITRO  RESEARCH USE ONLY


MOUSE MONOCLONAL CD3, conjugated to Fluorescein

PeliCluster  CD3 F

Cat#: RDI-M1466clb       PRICE: $375.00/vial

Test/vial 100


Clone CLB-T3/2, 16A9

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes. This antibody has been clustered to CD3 in one of the international Workshop on Human White Cell differentiation Antigens

Isotype The monoclonal antibody is of the mouse subclass IgG2a.


Source Ascites fluid of tumour bearing BALB/c mice.

Purification Ammoniumsulphate precipitation and ion exchange chromatography

Conjugation Conjugated with fluorescein iso thiocyanate isomer 1 (FITC).

Molecular F/P ratio between 5.5 - 9.5.

Packing Each vial contains 1 ml FITC conjugated monoclonal antibody and 10 mg BSA in PBS.

Preservative Mertiolate (0.001%).

Storage and stabilityMonoclonal antibodies should be stored at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.


Molecular mass 20, 25, 28 kD.


Application Monitoring of T-cell numbers in peripheral blood.

Characterization of leukaemias and lymphomas.


Methods Direct immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.


FOR IN VITRO RESEARCH USE ONLY


Mouse Monoclonal anti-CD3 (for T cell stimulation)


Cat#: RDI-M1654clb     $375.00/vial


Lot number:


Specificity:

Cell Type: Human T lymphocytes

Ag.MW. : 20,25, 28 kD.

Reactivity: The monoclonal antibody is directed against the CD- 3-antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells monocytes, granulocytes and platelets. The monoclonal antibody is in the presence of accessary cells mitogenic for resting T lymphocytes.

Application: To induce the proliferation of resting T lymphocytes.


Methods: Culture techniques.

Clone: 1XE

This clone is a switch-variant from the original clone 1X1, which has been derived from lybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human E-rozette-positive lymphocytes.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgE.

Source: TCS.

Packing: Each vial contains 0,1 ml  clarified TCS, 0.22u fitered (approx 150ug)

Storage: Monoclonal antibodies should be stored below -18C. Repeated thawing and freezing may reduce the antibody activity and should be avoided.Do not use this product beyond the stated expiration date.

FOR IN VITRO RESEARCH USE ONLY


Mouse ANTI-Human CD3 ANTIGEN

PRODUCT CODE :RDI-CBL150   $375.00/200ug vial - DISCONTINUED

Also available labeled with FITC  #RDI-CBL150FT   $375.00/100 tests

                                            PE   #RDI-CBL150PE   $469.00/100 Tests

                                          Biotin #RDI-CBL150BT   $375.00/100T

                                           R-PE-CY5 #RDI-CBL150PC5  $469.00/100T


CLONE: UCHT 1

ISOTYPE: IgG1

SOURCE: Mouse ascitic fluid

ANTIGEN: Purified human T Cells with P3X63Ag 8-653 as fusion partner

PURIFICATION METHOD: Ion exchange chromatography following ammonium sulphate precipitation.

SPECIFICITY: 22/26/30 kD MW lymphocyte surface molecules associated with the T cell antigen receptor complex. Reacts with 85% of peripheral blood T lymphocytes, 70% of thymocytes, the majority of T cell chronic lymphocytic leukaemias, Sezary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.

Antigen distribution: T-cells (E+) 100%

                               Peripheral blood lymphocytes 75 ± 7%

                               Thymocytes 68 ± 5%

                               Granulocytes < 1%

                               Monocytes < 1%

                                B-cells (E-) < 1%

                                NK-cells (CD16+) < 1%


APPLICATIONS:

* Identification of CD3 positive T lymphocytes by indirect immunofluorescence staining.

* Leukaemia and lymphoma phenotyping

* Identification of normal and malignant T lymphocytes in cryostat sections of frozen tissue

* Immunoprecipitation of the CD3 antigen

* Isolation or removal of T lymphocytes by FACS or panning techniques

* Mitogenic stimulation of T lymphocytes


REFERENCES: Leucocyte Typing III, Oxford University Press. (1987).

                          Beverley, P.C.L. & Callard, R.E. Eur. J. Immunol. 11,329-34 (1981)


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

BULK (NO BSA AND NO PRESERVATIVE= $1000.00/1mg ord cat#RDI-CBL150-1XP Larger sizes on request)


ANTI-CD3+ T lymphocyte receptor associated antigen

PRODUCT CODE : RDI-CBL 126     $375.00/vial 200Test/200ug

Also avaialble: FITC labeled   cat#RDI-CBL126FT   $375.00/vial 100 tests

                           PE labeled   cat#RDI-CBL126PE   $469.00/vial 100 tests

                        Biotin labeled cat#RDI-CBL126BT    $375.00/vial 100T

                         R-PE-CY5    cat#RDI-CBL126PC5   $469.00/100T


CLONE: Cris 7


ISOTYPE: IgG2a

SOURCE: Mouse ascitic fluid

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: 22/26/30 Kd MW lymphocyte surface molecule associated with the T cell antigen receptor complex and present on 85% of peripheral blood T lymphocytes, 70% of thymocytes, the majority of T cell chronic lymphocytic leukaemias, Sezary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin. The CD3 antigen is not present as a surface molecule on normal B lymphocytes, monocytes or granulocytes.

APPLICATIONS:

* Identification of CD3 positive T lymphocytes by indirect immunofluorescence staining.

* Leukaemia and lymphoma phenotyping

* Identification of normal and malignant T lymphocytes in cryostat sections of frozen tissue

* Immunoprecipitation of the CD3 antigen

* Isolation or removal of T lymphocytes by FACS or panning techniques

* Mitogenic stimulation of T lymphocytes


REFERENCES: LEUCOCYTE TYPING III (Oxford University Press) 1987, pp 42,43,957


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200ug/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20oC. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Bulk, No BSA/NO AZIDE cat#RDI-CBL126 -1XP $1000.00/milligram Larger on Request


ANTIBODY TO: HUMAN CD3

PRODUCT CODE:RDI-CBL 528  $375.00/200ug vial

CLONE  MEM 92

ISOTYPE  IgM

SOURCE  Mouse ascitic fluid

PURIFICATION METHOD  Ammonium sulphate precipitation followed by gel filtration chromatography

SPECIFICITY :This antibody recognizes 27/20 kDa molecular weight lymphocyte surface molecules associated with the T cell antigen receptor complex. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.

Antigen distribution:

T cells 100%

Peripheral blood lymphocytes 75 + 7%

Thymocytes 68 + 5%

Granulocytes < 1%

Monocytes < 1%

B cells (E-) < 1%

NK cells (CD16+) < 1%

APPLICATIONS

*Identification of CD3 positive T lymphocytes by indirect immunofluorescence staining

*Leukaemia and lymphoma phenotyping

*Identification of normal and malignant T lymphocytes in cryostat sections of frozen tissue

*Immunoprecipitation of the CD3 antigen

*Isolation and removal of T lymphocytes by FACS or panning techniques

REFERENCES

Leucocyte Typing IV, Oxford University Press (1989)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at +4oC or add an equal volume of glycerol and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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