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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD29 Antibodies

Mouse anti-human CD29 antibodies

- 3clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CBL481                 TDM29                           --           mIgG1       purified     $375.00

RDI-CBL481FT                " "                                 "              "             FITC        $375.00

RDI-CBL481PE               " "                                  "              "             PE            $438.00

RDI-CBL481BT               " "                                  "              "            Biotin         $375.00

RDI-CBL481PC5             ""                                    "              "           RPE/CY5   $469.00

RDI-M2129clb                2A4                               --              "             purified     $438.00

RDI-M2233clb                 " "                                 " --           "             FITC        $438.00

RDI-CD29abm-P4          P4C10                            --             mIgG1    purified     $500.00

RDI-CD29abm-4B         4B7R                             --              mIgG1    purified     $312.00

RDI-CD29abm-4BPE    4B7R                             --              mIgG1    PE             $500.00/120T

RDI-CD29abm-4BFT    4B7R                             --              mIgG1    FITC         $375.00/120T

RDI-CD29abm-4BBT   4B7R                             --              mIgG1    Biotin        $375.00/100ug

Product Specification: mouse monoclonal CD29

Code: CLB-CD29

CAT#: RDI-M2129clb   $438.00/vial

Lot Number:

Tests/vial: 200

Specificity:

Cell type: Broad leucocytes

Ag.MW.: 130 kD.

Reactivity: The monoclonal antibody is directed against the CD29-antigen (gplla, VLA B-chain,integrin B1- chain),which can form distinct complexes with either of six different a-chains of the Very Late Antigens (VLA). The monoclonal antibody reacts with thrombocytes, monocytes and a subpopulation of T and B lymfocytes. The monoclonal antibody does not react with erythrocytes and weak with granulocytes.

Application: Functional studies on cells.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: 2A4

This clone has been derived from hybridization of X63 Ag 865-3 cells with spleen cells of BALB/c mouse immunized with U937 cells.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.

Source: Mouse ascites fluid.

Purification: Protein G affinity chromatography.

Packing: Each vial contains 1 ml with approximately 200ug/ml monoclonal antibody and 1mg BSA in PBS.

Preservative: Sodium azide (0.1%).

Storage: Monoclonal antibodies should be stored at 2-8 Deg.C. Do not use this product beyond the stated expiration date.

Reference: 1.Humpries, M.J.,Cell Sci., 97,585-593 (1990).

FOR IN VITRO RESEARCH USE ONLY

Code: CLB-CD29-F

CAT#: RDI-M2233clb

Lot Number:

Tests/vial: 100

Specificity:

Cell type: Broad leucocytes

Ag.MW.: 130 kD.

Reactivity: The monoclonal antibody is directed against the CD29-antigen (gplla, VLA B-chain,integrin B1- chain),which can form distinct complexes with either of six different a-chains of the Very Late Antigens (VLA). The monoclonal antibody reacts with thrombocytes, monocytes and a subpopulation of T and B lymphcytes. The monoclonal antibody does not react with erythrocytes and weak with granulocytes.

Application: Functional studies on cells. Direct immunofluorescence staining with analysis by FACS.

purification: Protein G affinity chromatography.

Chemical Characteristics:

Molecular F/P ratio : 4.6

Packing: Each vial contains 1 ml conjugated monoclonal anti- body and 1 mg BSA in PBS.

Preservative: Sodium azide (0.1%).

Storage: Fluorescein labelled monoclonal antibodies should be stored at 2-8Deg.C and will retain their activity for approximately 12 months.

Reference: 1. Humpries, M.J.,Cell Sci., 97,585-592 (1990).

FOR IN VITRO RESEARCH USE ONLY

MOUSE MONOCLONAL ANTIBODY TO: HUMAN CD29

PRODUCT CODE   RDI-CBL481   $375.00/vial

CLONE    TDM29

ISOTYPE     IgG1

IMMUNIZING ANTIGEN   U937 cells

FUSION PARTNER   P3x63 Ag 8.653 myeloma cell line

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY    The antibody reacts with the integrin b1 chain which is a membrane glycoprotein (130 kDa) that can complex with the 6 different a-chains of the VLA's. It is also known as VLA b1-chain and the fibronectin receptor. The antigen is not expressed on erythrocytes and only weakly stains granulocytes.

Antigen distribution:

T cells             40 + 10%

CD4+ cells      40 + 20%

CD8+ cells      40 + 15%

B cells             20 + 5%

APPLICATIONS

*Immunoprecipitation studies of CD29 antigen

*Indirect immunofluorescence and FACS analysis for CD29 expressing cells

*Cell-cell and cell-matrix adhesion studies involving the CD29/CD49 heterodimers

REFERENCES

Hemler, M.E. Ann. Rev. Immunol. 8, 365-400 (1990)

Leucocyte Typing VI, Oxford University Press (in press)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available: FITC labeled cat#RDI-CBL481FT $375.00/100T vial

PE labeled cat#RDI-CBL481PE            $469.00/100T

Biotin labeled cat#RDI-CBL481BT      $375.00/100T

Bulk (purified, NO BSA) ord cat#RDI-CBL481-1X $1062.00/1mg

No BSA, NO AZIDE      cat#RDI-CBL481-1XP $1062.00/1mg

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

MOUSE ANTI-HUMAN INTEGRIN b1 (cd29)   MONOCLONAL ANTIBODY

CATALOG# RDI-CD29abm-P4    $500.00

Presentation: 100ug in 0.1ml (1mg/ml)

CLONE: P4C10

ISOTYPE: IgG1

SPECIFICITY: Reacts with the B1 subunit of the integrin protein family. Appears to be human specific.

APPLICATIONS: -Inhibits attachment of Mg63 cells to collagen type I at a dilution of 1:1500. Inhibits endodermal and keratinocytes to collagen,fibronectin and laminin. It also inhibits cell to cell adhesion.

-Immunoflourescence

-Immunoblotting

-Immunoprecipitation

-ELISA

Optimal working dilutions must be determined by end user.

STORAGE: Maintain at 2-8C in undiluted aliquots for up to 6 months.

REFERENCES: 1. Carter, W. A. et al. 1990. J. Cell Biol. 110:1387

                          2. Wayner, E.A. et al. 1991. J. Cell Biol. 113: 919.

For research use only; not for use as a diagnostic.

also available as ascites cat#RDI-CD29abm-P4AS $438.00/0.1ml

cat# RDI-CD29abm-4BPE   $500.00/120T

Presentation: 120Tests in 0.5ml 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Clone: 4B7R

Isotype: Mouse IgG1k

Immunogen: Human ocular melanoma cell line, V+B2

PRODUCTION: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.8 mg/ml with an A565/A280 ratio of 1.58.

DILUTION: 1:20 (80ul/test)

INFORMATION: Human CD29 is the beta subunit of an integrin family of molecules expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. CD29 can form heterodimer pairs with at least nine different alpha subunits. Antibody 4B7R recognizes the CD29 integrin subunit.

Activity: direct Flow cytometry

-in non-conjugated forms-Immunoprecipitation Frozen Sections

Fixed Formaldehyde Paraffin Sections

References: Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1612-1613.

STORAGE: Store at 2 - 5oC . Do not freeze! Protect from light.

PERFORMANCE: 500K cultured Nalm-6 cells were incubated 45 minutes on ice with 80 ul of anti-CD29/R-PE at a 1:20 dilution indicated above. Cells were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.75 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control (Cat#RDi- MIIG1PEX2 $188.00) at a similar concentration. Binding was 93% blocked when pre incubated 10 minutes with 20 ull of 0.5 mg/ml anti-CD29 antibody (Cat#RDI-CD29abm-4B)

Also available: intact

non conjugated cat#RDI-CD29abm-4B $312.00/100ug

FITC conjugated: cat#RDI-CD29abm-4BFT $375.00/120T

Biotin conjugated: cat#RDI-CD29abm-4BBT $375.00/100ug

PE conjugated: cat#RDI-CD29abm-4BPE $500.00/100T

-Phycoerythrin (R-PE) is covered under patents: U.S. 4,520,110; European 76,695 and Canadian 1,179,942.

BULK Material Quoted on Request

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

Ordering terms

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