rev: 5/16/2008
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD28
Mouse anti-human CD28 antibodies
-4 clones + polyclonals see also T cell stimulation series of antibodies (azide and BSA free)
& related CTLA-4, CD80 and CD86 antibodies
CATALOG# CLONE# Workshop Host Form Price
RDI-M1650clb CLB-CD28/1,15E8
V mIgG1
t cell stim $412.50
new cat#: 10R-CD28bHU
RDI-M1456clb CLB-CD28/1 , 15E8
V mIgG1
purified $412.50
new cat#: 10R-CD28aHU
RDI-M2241lb KOLT2 III mIgG1 purified $438.00
RDI-M2180clb " " " " FITC $438.00
Product Specification: mouse monoclonal anti-human CD28 T CELL STIMULATION
Code: CLB-CD28 (CLB-CD28/1)
CAT#: RDI-M1650clb $375.00/vial $312.00/vial 3 or
more
Specificity:
Cell type: Subpopulation of human T lymphocytes.
Ag.MW.: 44 kD.
Reactivity: The monoclonal antibody is directed against the CD28-antigen,which
is expressed on a subpopulation of human T-cells (1) and activated B-cells. It
has ben shown that CD28 positive CD8-cells are cytotoxic T lymphocyte
precursors. The monoclonal antibody does not react with B-cells, granulocytes
and monocytes.
Application: To induce the proliferation of resting T lymphocytes.
Methods: Culture techniques.
Clone: 15E8 (402)
This clone has been derived from hybridization of SP2/0 cells with spleen
cells of a BALB/c mouse immunized with human T lymphocytes.
Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.
Source: Ascites fluid of tumor bearing BALB/c mice.
Purification: Ammoniumsulphate precipitation and ion exchange chromatography.
Packing: Each vial contains 0.1 ml with approximately 2.0mg/ ml monoclonal
antibody in 20mM TRIS and 150 mM NaCI, pH 8.0 filtered 0.22um.
Storage: Monoclonal antibodies should be stored below -18Deg..Repeated thawing
and freezing may reduce the antibody activity and should be avoided.Do not use
this product beyond the stated expiration date.
Reference: 1. Lier,R.A.W. van, et al.,Knapp,W.(editor),
Leucocytel Typing IV, Oxford University Press, chapter 2, 353 (1989).
FOR IN VITRO RESEARCH USE ONLY
Product Specification: mouse monoclonal anti-human CD28
PeliCluster CD28
CAT#RDI- M1456clb
Test/vial 200
Clone CLB-CD28/1, 15E8
This clone has been derived from hybridization of SP2/0 cells with spleen cells
of a BALB/c mouse immunized with human T lymphocytes. This antibody has been
clustered to CD28 in one of the international Workshop on Human White Cell
differentiation Antigens.
Isotype Mouse IgG1.
Source Ascites fluid of tumour bearing BALB/c mice.
Purification Ammoniumsulphate precipitation and ion exchange chromatography
Packing Each vial contains 1 ml with approximately 0.2 mg/ml monoclonal
antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.
Preservative Mertiolate (0.001%).
Storage and stability Monoclonal antibodies should be stored in the dark at
2-8°C. The reagent is stable until the expiry date stated on the vial
label.
Major reactivity The monoclonal antibody is directed against the CD28- antigen,
which is expressed on a subpopulation of human T-cells (1) and activated
B-cells. It has been shown that CD28 positive CD8-cells are cytotoxic T lymphocyte
precursors. The monoclonal antibody does not react with B-cells, granulocytes
and monocytes.
Molecular mass 44 kDa.
Application To analyze the role of CD28 in T-cell proliferation (2, 3).
Methods Indirect immunofluorescence staining with analysis by flowcytometry or
fluorescence microscopy.
References
1. Lier, R.A.W. van, et al., Knapp, W. (editor), Leucocyte Typing IV, Oxford University Press, chapter 2, 353 (1989).
2. Lier, R.A.W. van, et al., McMichael, A.J. (editor), Leucocyte Typing III, Oxford University Press, chapter 6, 170 1987).
3. Lier, R.A.W. van, et al., Eur. J. Immunol., 18, 167 (1988).
APPLICATION FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE
Reagents
- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).
Remove aggregates by centrifugation at 1000 x g for 10 minutes.
- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).
- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.
- microplate plates (96 wells, V bottom) or tubes.
Procedure
1 Fixate the cells with PFA 1% and wash them with buffer.
2 Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.
3 Add 45 µl of cell suspension to the microplate wells or tubes.
4 Add 5 µl monoclonal antibody to the microplate wells or tubes and mix gently.
5 Incubate for 30 minutes at 2-8 C.
6 Add 100 µl buffer to the microplate wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
7 Aspirate the supernatant from the cell pellet and resuspend the cells.
8 Add 200 µl buffer to the microplate wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.
9 Aspirate the supernatant from the cell pellet and resuspend the cells.
10 Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human immunoglobulins, diluted 1:80.
11 Incubate for 30 minutes at 2-8 C.
12 Repeat step 6-9.
13 Add 200 µl buffer.
14 The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.
NOTE: Care should be taken when drawing blood to avoid activation of platelets.
Platelets activation markers are weakly expressed on platelets prepared from
directly fixed normal whole blood.
The expression is maximal on thrombin (1 U/ml) stimulated washed platelets.
FOR IN VITRO RESEARCH USE ONLY
Product Specification: mouse monoclonal anti-human CD28
Code: CLB-CD28
CAT#: RDI-M2241clb $438.00/vial
Lot Number:
Tests/vial: 200
Specificity:
Cell type: Subpopulation of human T lymphocytes
Ag.MW.: 44 kD.
Reactivity: The monoclonal antibody is directed against the CD28-antigen,which is weakly expressed on T-cells in the resting period and strongly on human T-cells which have been stimulated by mitogens like PHA or ConA.
The monoclonal antibody also reacts with 30% of human thymocyts, adult
T-cell leukaemia cells and human monocytes. The monoclonal does not react with
normal human B lymphocytes,granulocytes,thrombocytes,erythrocytes, common acute
lymphoblastic leukaemia cells or acute myelocytic leukaemia cells.
Application: Identification of activated T-cells. Analysis of T-cell activation
mechanism.
Methods: Indirect immunofluorescence staining with analysis by FACS or
fluorescence microscopy.
Clone: KOLT2
Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.
Source: Hybridoma cultured supernatant.
Purification: Protein A affinity chromatography.
Packing: Each vial contains 1 ml with approximately 50ug/ml monoclonal antibody
and 10 mg BSA in PBS.
Preservative: Merthiolate (0.001%).
Storae: Monoclonal antibodies should be stored below -18 Deg.. Repeated
thawing and freezing may reduce the antibody activity and should be avoided.
FOR IN VITRO RESEARCH USE ONLY
Product Specification: mouse monoclonal CD28,conjugated to Fluorescein
Code: CLB-CD28-F
CAT#: RDI-M2180clb $438.00/vial
Specificity:
Cell type: Subpopulation of human T-lymphcytes
Ag.MW.: 44 kD.
Reactivity: The monoclonal antibody is directed against the CD28-antigen, which
is weakly expressed on T-cells in the resting period and strongly on human T-
cells, which have been stimulated by mitogens like PHA or ConA. The monoclonal
antibody also reacts with 30% of human thymocytes and adult T-cell leukaemia
cells. The monoclonal does not react with normal human B lymphocytes,
monocytes, granulocytes,platelets, erythrocytes,common acute lymphoblastic
leukaemia cells or acute myelocytic leukaemia cells.
Application: Identification of activated T-cells. Analysis of T-cell activation
mechanism.
Methods: Direct immunofluorescence staining with analysis by FACS or
fluorescence microscopy.
Clone: CLB-KOLT-2
Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.
Source: Hybridoma cultured supernatant.
Purification: Protein A affinity chromatography.
Chemical Characteristics:
Molecular F//P ratio : 8.7
Weight F/P Ratio : 2.3 x 10-2
Fluorescein concentration : 1.1 x 10-3
Packing Each vial contains 1 ml with approximately 25ug/ml conjugated
monoclonal antibody and 10mg BSA in PBS.
Preservative: Merthiolate (0.001%).
Storage: Monoclonal antibodies should be stored below -18 Deg..Repeated thawing
and freezing may reduce the antibody activity and should be avoided.
FOR IN VITRO RESEARCH USE ONLY
-All products are for in vitro research use only-Not for use in or on humans or
animals-Not for Diagnostic use. Not responsible for any patent infringements
with the use or derivation of these products. Price/specifications/availability
subject to change without notice.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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