rev:  July 29, 2002

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD28 Antibodies

Mouse anti-human CD28 antibodies

-4 clones   +   polyclonals  see also T cell stimulation series of antibodies (azide and BSA free)

     &  related        CTLA-4,   CD80 and   CD86   antibodies

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-M1650clb             CLB-CD28/1,15E8         V             mIgG1      t cell stim  $375.00

RDI-M1456clb             CLB-CD28/1 , 15E8       V             mIgG1      purified     $375.00

RDI-M2241lb               KOLT2                           III            mIgG1      purified     $438.00

RDI-M2180clb                " "                                 "                 "            FITC        $438.00

Product Specification: mouse monoclonal anti-human CD28   T CELL STIMULATION


Code: CLB-CD28 (CLB-CD28/1)


CAT#: RDI-M1650clb    $375.00/vial    $312.00/vial 3 or more


Specificity:

Cell type: Subpopulation of human T lymphocytes.

Ag.MW.: 44 kD.

Reactivity: The monoclonal antibody is directed against the CD28-antigen,which is expressed on a subpopulation of human T-cells (1) and activated B-cells. It has ben shown that CD28 positive CD8-cells are cytotoxic T lymphocyte precursors. The monoclonal antibody does not react with B-cells, granulocytes and monocytes.


Application: To induce the proliferation of resting T lymphocytes.


Methods: Culture techniques.


Clone: 15E8 (402)

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human T lymphocytes.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.


Source: Ascites fluid of tumor bearing BALB/c mice.


Purification: Ammoniumsulphate precipitation and ion exchange chromatography.


Packing: Each vial contains 0.1 ml with approximately 2.0mg/ ml monoclonal antibody in 20mM TRIS and 150 mM NaCI, pH 8.0 filtered 0.22um.

Storage: Monoclonal antibodies should be stored below -18Deg..Repeated thawing and freezing may reduce the antibody activity and should be avoided.Do not use this product beyond the stated expiration date.



Reference: 1. Lier,R.A.W. van, et al.,Knapp,W.(editor),

                     Leucocytel Typing IV, Oxford University Press, chapter 2, 353 (1989).


FOR IN VITRO RESEARCH USE ONLY

Product Specification: mouse monoclonal anti-human CD28


PeliCluster  CD28

CAT#RDI- M1456clb

Test/vial 200

Clone CLB-CD28/1, 15E8
This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human T lymphocytes. This antibody has been clustered to CD28 in one of the international Workshop on Human White Cell differentiation Antigens.

Isotype Mouse IgG1.


Source Ascites fluid of tumour bearing BALB/c mice.


Purification Ammoniumsulphate precipitation and ion exchange chromatography

Packing Each vial contains 1 ml with approximately 0.2 mg/ml monoclonal antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.


Preservative Mertiolate (0.001%).


Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD28- antigen, which is expressed on a subpopulation of human T-cells (1) and activated B-cells. It has been shown that CD28 positive CD8-cells are cytotoxic T lymphocyte precursors. The monoclonal antibody does not react with B-cells, granulocytes and monocytes.

Molecular mass 44 kDa.

Application To analyze the role of CD28 in T-cell proliferation (2, 3).


Methods Indirect immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.

References

1. Lier, R.A.W. van, et al., Knapp, W. (editor), Leucocyte Typing IV, Oxford University Press, chapter 2, 353 (1989).

2. Lier, R.A.W. van, et al., McMichael, A.J. (editor), Leucocyte Typing III, Oxford University Press, chapter 6, 170        1987).

3. Lier, R.A.W. van, et al., Eur. J. Immunol., 18, 167 (1988).



APPLICATION FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE

Reagents

- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).

   Remove aggregates by centrifugation at 1000 x g for 10 minutes.

- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).

- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.

- microplate plates (96 wells, V bottom) or tubes.

Procedure

1 Fixate the cells with PFA 1% and wash them with buffer.

2 Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.

3 Add 45 µl of cell suspension to the microplate wells or tubes.

4 Add 5 µl monoclonal antibody to the microplate wells or tubes and mix gently.

5 Incubate for 30 minutes at 2-8 C.

6 Add 100 µl buffer to the microplate wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

7 Aspirate the supernatant from the cell pellet and resuspend the cells.

8 Add 200 µl buffer to the microplate wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

9 Aspirate the supernatant from the cell pellet and resuspend the cells.

10 Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human immunoglobulins,     diluted 1:80.

11 Incubate for 30 minutes at 2-8 C.

12 Repeat step 6-9.

13 Add 200 µl buffer.

14 The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.


NOTE: Care should be taken when drawing blood to avoid activation of platelets.

Platelets activation markers are weakly expressed on platelets prepared from directly fixed normal whole blood.

The expression is maximal on thrombin (1 U/ml) stimulated washed platelets.

FOR IN VITRO RESEARCH USE ONLY

Product Specification: mouse monoclonal anti-human CD28

Code: CLB-CD28


CAT#: RDI-M2241clb   $438.00/vial

Lot Number:

Tests/vial: 200

Specificity:

Cell type: Subpopulation of human T lymphocytes

Ag.MW.: 44 kD.

Reactivity: The monoclonal antibody is directed against the CD28-antigen,which is weakly expressed on T-cells in the resting period and strongly on human T-cells which have been stimulated by mitogens like PHA or ConA.

The monoclonal antibody also reacts with 30% of human thymocyts, adult T-cell leukaemia cells and human monocytes. The monoclonal does not react with normal human B lymphocytes,granulocytes,thrombocytes,erythrocytes, common acute lymphoblastic leukaemia cells or acute myelocytic leukaemia cells.

Application: Identification of activated T-cells. Analysis of T-cell activation mechanism.


Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: KOLT2

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.

Source: Hybridoma cultured supernatant.


Purification: Protein A affinity chromatography.


Packing: Each vial contains 1 ml with approximately 50ug/ml monoclonal antibody and 10 mg BSA in PBS.


Preservative: Merthiolate (0.001%).

Storae: Monoclonal antibodies should be stored below -18 Deg.. Repeated thawing and freezing may reduce the antibody activity and should be avoided.

FOR IN VITRO RESEARCH USE ONLY

Product Specification: mouse monoclonal CD28,conjugated to   Fluorescein


Code: CLB-CD28-F


CAT#: RDI-M2180clb     $438.00/vial

Lot Number:

Specificity:

Cell type: Subpopulation of human T-lymphcytes

Ag.MW.: 44 kD.


Reactivity: The monoclonal antibody is directed against the CD28-antigen, which is weakly expressed on T-cells in the resting period and strongly on human T- cells, which have been stimulated by mitogens like PHA or ConA. The monoclonal antibody also reacts with 30% of human thymocytes and adult T-cell leukaemia cells. The monoclonal does not react with normal human B lymphocytes, monocytes, granulocytes,platelets, erythrocytes,common acute lymphoblastic leukaemia cells or acute myelocytic leukaemia cells.


Application: Identification of activated T-cells. Analysis of T-cell activation mechanism.


Methods: Direct immunofluorescence staining with analysis by FACS or fluorescence microscopy.


Clone: CLB-KOLT-2

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.


Source: Hybridoma cultured supernatant.


Purification: Protein A affinity chromatography.


Chemical Characteristics:

Molecular F//P ratio : 8.7

Weight F/P Ratio : 2.3 x 10-2

Fluorescein concentration : 1.1 x 10-3


Packing Each vial contains 1 ml with approximately 25ug/ml conjugated monoclonal antibody and 10mg BSA in PBS.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18 Deg..Repeated thawing and freezing may reduce the antibody activity and should be avoided.


FOR IN VITRO RESEARCH USE ONLY


-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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