rev: November 27, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD26 Antibodies

Mouse anti-human CD26 antibodies

-2 clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-M1696clb                 CLB-22C3                  --            mIgG1      purified      $375.00

RDI-CD26abm-36            202.36                        --            mIgG2B    purified      $312.00

PeliCluster  CD26

CAT#RDI- M1696clb

Test/vial 200

Clone CLB-22C3
This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with a human T-cell clone. This antibody has been clustered to in one of the international Workshop on Human White Cell differentiation Antigens

Isotype Mouse, IgG1.

Source Ascites fluid of tumour bearing BALB/c mice.

Purification Ammoniumsulphate precipitation and ion exchange chromatography.

Packing Each vial contains 1 ml with approximately 0.2 mg/ml monoclonal antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.

Preservative Mertiolate (0.001%).

Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD26- antigen, which is expressed on human activated T- and B- cells.

Molecular mass 120 kDa.

Application Detection of activated T- and B-cells.

Methods Indirect immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.

APPLICATION FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE

Reagents

- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).

   Remove aggregates by centrifugation at 1000 x g for 10 minutes.

- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).

- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.

- Microtiter plates (96 wells, V bottom) or tubes.

Procedure

1 Fixate the cells with PFA 1% and wash them with buffer.

2 Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.

3 Add 45 µl of cell suspension to the microtiter wells or tubes.

4 Add 5 µl monoclonal antibody to the microtiter wells or tubes and mix gently.

5 Incubate for 30 minutes at 2-8 C.

6 Add 100 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

7 Aspirate the supernatant from the cell pellet and resuspend the cells.

8 Add 200 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

9 Aspirate the supernatant from the cell pellet and resuspend the cells.

10 Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human immunoglobulins,     diluted 1:80.

11 Incubate for 30 minutes at 2-8 C.

12 Repeat step 6-9.

13 Add 200 µl buffer.

14 The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.

NOTE: Care should be taken when drawing blood to avoid activation of platelets.

Platelets activation markers are weakly expressed on platelets prepared from directly fixed normal whole blood.

The expression is maximal on thrombin (1 U/ml) stimulated washed platelets.

FOR IN VITRO RESEARCH USE ONLY

PRODUCT CODE RDI-CBL513   $375.00/vial 200ug

Also available: FITC labeled cat#RDI-CBL513FT   $375.00/100 test vial

                           PE labeled cat#RDI-CBL513PE   $469.00/100 test vial

CLONE: 134-2C2

ISOTYPE: IgM

SOURCE: Tissue culture supernatant

Antigen: CD3 activated PBMC

Fusion Partner: NS1 myeloma cell line

PURIFICATION METHOD: ammonium sulphate precipitation followed by gel filtration

SPECIFICITY: This antibody reacts with the 110kDa T cell activation antigen that has cell surface dipeptidyl peptidase Iv (DPPIV) enzyme activity. The CD26 antigen is a functional collagen receptor as well as a DPPIV ectoenzyme which cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position.

APPLICATIONS:

*Studies into the nechanism of CD26 mediated T cell signalling and the asociation with CD45 tyrosine phosphatase and adenosine deaminase.

-Studies into the possible involvement of CD26 in HIV infection in particular the putative suggestion that CD26 is a co-receptor for HIV.

REF: -Leucocyte Typing IV, Oxford Universiity Press (1989)

         -Leucocyte Typing V, Oxford University Press (in press)

         -D'ang ,N.H. et al, J. Exp. Med 172, 649-652 (1990)

         -Tommoto, Y. et al, Mol. Immunol 29, 183-192 (1992)

         -Marimoto C. and Schlossmon, S.F. Immunologist 2, 4-7 (1994)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME

cat# RDI-CD26abm-36         $312.00/100ug

       RDI-CD26abm-36FT     $375.00/120T FITC

RDI-CD26abm-36BT            $375.00/100ug Biotin

RDI-CD26abm-36PE             $500.00/120T PE

Presentation: 100ug in 0.1ml (1mg/ml) 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative). BULk no Preservative on request

Clone: 202.36

Isotype: Mouse IgG2b

Immunogen: Human T cell clone

PRODUCTION: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.

Use: -Flow cytomery

       -histochemistry(aceotne fixed frozen sections)

        -immunoprecipitation

STORAGE CONDITIONS: Store at 2 - 5oC. Freeze/Thawing is not recommended.

PERFORMANCE: Briefly, ficoll prepared human peripheral blood leukocytes were stimulated in culture 1 day with 10 ng/ml PMA and 1 mM Ionomicin. 5 x 105 cells per tube were washed and preincubated ~5 minutes with 20 ul of human IgG at 250 ug/ml (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD26 antibody at 10 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed using a BD FACS Calibur. A net 24% population of the cells stained positive with a mean shift of 0.31 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration.

Background: Human CD26 is a T cell activation antigen which can be associated with costimulation (1). The significance of the dipeptidyl peptidase IV activity remains unknown. Recent characterization of adenosine deaminase binding to CD26 suggests an important immunoregulatory role (2). Antibody 202.36   reacts with the type II membrane protein   CD26.

References: 1.) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1105-1114. 2.) R.P. Dong, et al, (1996) J. Immunol 156: 1349-1355.

*This Product is intended for Laboratory Research use only.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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