rev:  May 1, 2003

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD21 Antibodies

Mouse anti-human CD21 antibodies

-2 clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CD21abm033        BU33                           V               mIgG1      see below

RDI-M1723clb              CLB-5D1                     --                             purified     $375.00

RDI-M2273clb             WEHI-B2                      --                              FITC        $375.00

catalog#

non conjugated   cat#RDI-CD21abm-33          $312.00/100ug

FITC conjugated: cat#RDI-CD21abm-3FT     $375.00/120T

Biotin conjugated: cat#RDI-CD21abm-3BT   $375.00/100ug

PE conjugated: cat#RDI-CD21abm-3PE        $500.00/100T

Presentation: 100ug in 0.1ml (1mg/ml) 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5mg/ml Gentamicin Sulfate

Clone: BU33

Isotype: Mouse IgG1

Immunogen: Human HFB-1 cell line

PRODUCTION: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.

INFORMATION: Human CD21 is a receptor for complement fragments C3d, C3dg or ic3D and also EBV . CD21 is also a ligand for CD23 and plays a role in IgE synthesis. Antibody BU33 recognizes the CD21 molecule of about 145 kd.Antibody BU33 inhibits binding to CD23.

Uses: Immunochemistry Applications:

         Flow cytometry

         Immunoprecipitation

         Frozen Sections

         Western Blot

References: Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 516-523. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 535-538. J.Y. Bonnefoy, et al, (1995) Intl Archiv Allergy Immunol 107: 40-42. A.D. Beaulieu, et al, (1995) Blood 86: 2789-2798. H. Molina, et al, (1995) J Immunol 154: 5426-5435.

STORAGE: Store at 2 - 5oC. Freeze/Thawing is not recommended.

PERFORMANCE: 500K cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-human CD21 (CR2) at 20 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC , after which they were washed three times, fixed and analyzed continued on back side using a BD FACstar plus. Cells stained positive with a mean shift of 1.17 log10 fluorescent units when compared to a Mouse IgG1 negative control (Catalog #RDI-MIGG1X2, $188.00/0.1mg) at a similar concentration.

*This Product is intended for in vitro research use only-not for use in or on humans or animals.

Art.no RDI-M2273clb

Specification sheet

Test/vial 100

Clone WEHI-B2 (B21)

This antibody has been clustered toCD21 in the fourth international Workshop on Human White Cell differentiation Antigens in Vienna.

Isotype Mouse, IgG2a.

Source Mouse ascites fluid.

Purification Ammoniumsulphate precipitation followed by ion exchange chromatography.

Conjugation Conjugated with fluorescein iso thiocyanate isomer 1 (FITC).

Molecular F/P ratio between 5.5 - 9.5.

Packing Each vial contains 1 ml FITC conjugated monoclonal antibody 2 mg BSA in PBS.
Preservative 0.1% NaN3.

Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD21-antigen, which is expressed on normal Ig-positive B-cells from peripheral blood and lymphoid tissues and on dendritic cells of germinal centres. Its expression is lost on activated B-cells. The distinct distribution among B-cell malignancies differs from other B-cell markers e.g. CD19 and CD20.

The monoclonal antibody reacts positive with some cases of childhood T-cell acute lymphoblastic leukaemia (T-ALL).

The monoclonal antibody does not react with mature T- and NK-cells and non lymphoid haemopoietic cells.

Molecular mass 140-145 kDa.

Application Identification of CD21 positive lymphocytes cells.

Identification of normal and malignant CD21 positive B-lymphocytes on frozen sections.

Methods Direct immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.

Reference 1. Knapp, W. et al. (editor), Leucocyte typing IV, White cell differentiation antigens, Oxford (1989).

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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