rev: October 3, 2001

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD166 Antibodies
Mouse anti-human CD166  antibodies

CATALOG#                 CLONE#                  Workshop    Host         Form            Price

RDI-CD166-3A6            3A6                             VI               mIgG1      purified       $344.00/100ug

RDI-CD166-3FT                                                                                  FITC            $375.00/100T

RDI-CD166-3BT                                                                                  Biotin          $375.00/100ug

  polyclonals   &Bulk quotes on request

Mouse anti-human CD166 (ALCAM) antibodies

RDI-CD166-3A6       $344.00/100ug

Package Size: 100ug in 0.1ml (1mg/ml) 50mM sodium phosphate pH7.5, 1000mM Potassium Chloride, 150mM NaCl,, 0.5mg/ml gentamicin sulfate.

Prep: purified by Protein A from TCS. Purity >95% by SDS-Page.

Species: mouse IgG1k

Immunogen: Human thymic epithelial cells

CLONE: 3A6

Workshop: VI, Code#A109

Activity: Reacts with human CD166 (Alcam, activated leucocyte cell adhesion molecule. mr 100,000 with five Ig-like domains), a type 1 glycoprotein expressed on activated T cells, B cells and monocytes and appears to be the ligand for CD6. Human CD166 may be important for activation of T cells. Alcam is a ligand for CD6 and Mab from clone 3A6 blocks binding of CD6 to CD166.
-tissue distribution:activated T and B cells, thymic epithelium, hassal's bodies, fibroblasts, endothelial cells, epidermal keratinocytes and neurons.

-histochemistry (acetone fixed frozen sections)

-immunoprecipitation

-blocks binding of CD6 to CD166

References: 1) Leucocyte Typing VI, Garland Press, NY 1997

                    2) J EXp Med 181:2213-2220 (1995)

                    3) J Biol Chem 271:17390-17396 (1996)

Application: 500K cultured Raji cells per tube were washed and preincubated 5 minutes with 20ul of 250ug/ml human IgG(to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of the anti-CD166 antibody at a concentration of 5ug/ml. Cells were washed twice and incubated with 50ul of secondary reagent Goat anti-mouse IgG:FITC, after which they were washed three times, fixed and analyzed using a BD FACSCalibur. Cells stained positive with a mean shift of 0.87 log 10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration.

Storage: Store at 4 DEG C.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Mouse anti-human CD166 (ALCAM) antibodies  FITC conjugated

RDI-CD166-3FT   $375.00/120T FITC conj

ackage Size: 120Tests in 0.2ml 50mM sodium phosphate pH 7.5, 1000mM Potassium Chloride, 150mM NaCl, 5% glycerol, 0.2% BSA & 0.04% NaN3.


Prep: Protein A purified antibody from TCS conjugated to FITC. Unconjugated FITC removed by desalting column. F:P ratio 5.9

Species: mouse IgG1k

Immunogen: Human thymic epithelial cells

CLONE: 3A6

Workshop: VI, Code#A109

Activity: Reacts with human CD166 (Alcam, activated leucocyte cell adhesion molecule. mr 100,000 with five Ig-like domains), a type 1 glycoprotein expressed on activated T cells, B cells and monocytes and appears to be the ligand for CD6. Human CD166 may be important for activation of T cells. Alcam is a ligand for CD6 and Mab from clone 3A6 blocks binding of CD6 to CD166.

Sample Protocol: 500K cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of the anti-CD166:Fitc conjugated diluted 1:50. Cells were washed three times, fixed and analyzed by Facs. Cells stained positive with a mean shift of 0.71log10 fluorescent units when compared to the mouse IgG1:FITC negative control. Binding was blocked 98% when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD166 purified antibody.

negative control: MIgG1:FITC

cat#RDI-MOPC31CFT $156.00/100ug $125.00/vial 2-4


References: 1) Leucocyte Typing VI, Garland Press, NY 1997

                    2) J EXp Med 181:2213-2220 (1995)

                     3) J Biol Chem 271:17390-17396 (1996)

Storage: Store at 4-8 DEG C. DO NOT fREEZE. Protect   From Light.

Precautions:For In vitro research Use Only. Not for use in or onhumans or animals or for diagnostics. It is the responsibility ofthe user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patentinfringements that might result with the use of or derivation of this product.

Mouse anti-human CD166 (ALCAM) antibodies :Biotin conjugated

RDI-CD166-3BT $375.00/100ugBiotin conj

Package Size: 100ug in 0.1ml 50mM sodium phosphate pH 7.5, 1000mM Potassium Chloride, 150mM NaCl, 5% glycerol, 0.2% BSA & 0.04% NaN3.

Prep: Protein A purified antibody from TCS and conjugated to Biotin. Unconjugated Biotin removed by desalting column.

Species: mouse IgG1k

Immunogen: Human thymic epithelial cells

CLONE: 3A6

Workshop: VI, Code#A109

Activity: Reacts with human CD166 (Alcam, activated leucocyte cell adhesion molecule. mr 100,000 with five Ig-like domains), a type 1 glycoprotein expressed on activated T cells, B cells and monocytes and appears to be the ligand for CD6. Human CD166 may be important for activation of T cells. Alcam is a ligand for CD6 and Mab from clone 3A6 blocks binding of CD6 to CD166.

-Sample Protocol: 500K cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of the anti-CD166:Biotin conjugated at 10ug/ml. Cells   were washed twice and incubated with 20ul of secondary reagent Streptavidin:PE after which they were washed three times, fixed and analyzed by Facs. Cells stained positive with a mean shift of 1.70 log10 fluorescent units when compared to the mouse IgG1:Biotin negative control. Binding was blocked 93% when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD166 purified antibody.

Streptavidin:PE cat#RDI-STREPPEX  $156.00/120T    $125.00/vial 3+

Negative control/ mouse IgG1:Biotin  cat#RDI-MOPC31CBT  $156.00   $125.00/vial 2-4



References: 1) Leucocyte Typing VI, Garland Press, NY 1997

                    2) J EXp Med 181:2213-2220 (1995)

                     3) J Biol Chem 271:17390-17396 (1996)

Storage: Store at 4-8 DEG C. DO NO fREEZE.

Precautions:For In vitro research Use Only. Not for use in or onhumans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.
RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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