rev: March 26, 2002

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD141 (Thrombomodulin/TM) Antibodies

-see 2 clones below

PRODUCT CODE                   RDI-CBL584      $375.00

CLONE                                   B-A35

ISOTYPE                                IgG1

IMMUNIZING ANTIGEN      TF1 cell line

FUSION PARTNER               X63/Ag.8653 myeloma cell line

SOURCE                                 Tissue culture supernatent

PURIFICATION METHOD   Ion-exchange chromatography

SPECIFICITY                        The antibody recognises the single chain 75kDa polypeptide that is thrombomodulin expressed on human endothelium. The antibody also recognises the soluble form of the antigen.

APPLICATIONS *Flow cytometry

CBL584 Cont'dPRESENTATION The monoclonal is presented as  200ug in 2ml100 tests vial (1ml) in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

cat# RDI-CD141abm-C01       $438.00/0.5ml

       RDI-CD141abm-CO1X   $625.00/1ml

Presentation:              Tissue culture supernatant with 0.09% sodium azide

Immunogen:                Recombinant protein encoding the six repeated EGF domains of human thrombomodulin.

Mol. Wt. of Antigen:  75kDa

Isotype:                     mIgG1

Clone:                       141C01

Reactivity:                 human, rat, (others not tested)

Uses:                        -histochemistry (frozen and paraffin sections) approx 1:25-1:50 for 60 minutes RT

                                 -for paraffin sections, requires boiling tissue sections in 10mM cirate buffer pH6.0 for 10-20

                                 minutes followed by cooling at RT for 20 minutes.

Positive Control: Mesothelioma

Localization: Cell membrane

References:            1. Fink L, et al. Intl J Develop Biol 1993; 37:221

                               2. Ford VA, et al. J Biol Chem 1992; 267:5446

                                3. Collins CL, et al. Amer J Pathol 1992; 141(4): 827

Background: Thrombomodulin (TM) is also known as Fetomodulin (FM),   endothelial anticoagulant protein, or glycoprotein P112.   Thrombomodulin contains six repeated domains   homologous with epidermal growth factor (EGF) and   an amino terminal domain homologous to lectin-like   protein. Through its accelerated activation of protein   C, synthesis of TM is one of several mechanisms   important in maintaining thrombo-resistance and thus   reducing clot formation on the surface of the   endothelial cells. Immunohistochemical staining of   formalin-fixed tissue for TM has been used for the   study of a variety of vascular tumors, meningiomas   and choriocarcinomas.

Storage: 2-8 deg c

For Research Use Only

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222 or

           (978) 371-6446 or (800) 370-2222

fax      (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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