rev:     February 2, 2005

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD11c Antibodies


Mouse anti-human CD11c antibodies

-2 clones     3.9 (purified, FITC or PE)

                    HC1/1 (purified, FITC , PE or Biotin  )


CATALOG#                 CLONE#                  Workshop  Host         Form        Price

RDI-CBL146                 3.9                              III & IV    mIgG1      purified      $375.00

RDI-CD11cabm-39FT   " "                                " "               "            FITC         $375.00

RDI-CD11cabm-39PE   " "                                " "              "             PE            $500.00

RDI-CD11cabm-39BT    " "                               " "              "             Biotin       $375.00/100ug


Mouse ANTI-HUMAN CD11c MONOCLONAL ANTIBODY


PRODUCT CODE :RDI-CBL146     $375.00/200ug vial

    cat#RDI-CD11Cabm-39FT  $375.00/vial 120T FITC labeled

    cat#RDI-CD11Cabm-39BT   $375.00/100ug Biotin conjugated

         #RDI-CD11Cabm-39PE   $500.00/vial of 120 tests PE labeled



CLONE: 3.9

ISOTYPE: IgG1

SOURCE: Mouse ascitic fluid


PURIFICATION METHOD: Ammonium sulphate precipitation followed by ion exchange chromatography


SPECIFICITY: The antibody reacts with the ax chain of the CD11c / CD18 (p150 / 95) integrin heterodimer expressed on monocytes and weakly on granulocytes The antibody stains macrophages in most types of tissues. In flow cytometry the antibody labels a T-cell subset and weakly a B-cell subset.

Antigen distribution: Monocytes > 98%

                               Granulocytes 33 ± 12%

                               LGL cells < 5%

                               Peripheral blood lymphocytes < 1%

                               T-cells (CD3+) < 1%

                               B-cells (CD20+) < 1%

                               Thymocytes < 1%

APPLICATIONS:

* The antibody can be used to detect LFA deficiency.

* Characterisation of leukaemias and lymphomas.

* Detection of macrophages in frozen tissue.

REFERENCES: Hogg, N. Eur. J. Immunol. (1986) 16, 240.

                          Leucocyte Typing III, Oxford University Press. (1987).

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Mouse anti-human CD11C:FITC conjugated clone 3.9

cat# RDI-CD11Cabm-39FT   $375.00

Presentation: 120 Tests in 0.2ml 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. 5% glycerol, 0.2% BSA and 0.04% sodium azide.

Clone: 3.9

Isotype: Mouse IgG1

Immunogen: Human rheumatoid synovial fluid cells/monocytes.

PRODUCTION: Antibody was Protein A purified from tissue culture supernatant and conjugated to FITC. Unconjugated FITC separated by desalting column. F:P 7.4

PERFORMANCE: Five x 105 ficoll prepared human peripheral white blood cells were washed and preincubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding), after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD11c:FITC at 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed using a BD FACS Calibur. A net 12.5% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1:FITC negative control at a similar concentration.

INFORMATION: Human CD11c (alpha X integrin) complexes with CD18 (beta2 integrin) to form the complement receptor type 4 (CR4) heterodimer which binds to fibrinogen and is involved with monocyte/granulocyte adhesion during inflammatory responses. CD11c expression is restricted to leukocytes mainly of myeloid lineage with highest expression on macrophages. Antibody 3.9 recognizes the integrin alpha X subunit (CD11c) of about 150 kd.

References: N. Hogg,et al, (1986) Eur J Immunol 16: 240-248.

S.A. Stacker & T.A. Springer, (1991) J Immunol

146: 648-655. M. Patarroyo, (1994) Immunobiology

191: 474-477. Leukocyte Typing V (1995) (S.F. Schlossman, et al., eds.) Oxford University Press, Oxford, p. 1590-1592.

Recommended FITC Isotype Control

cat#RDI-MIGG1FTX2 $188.00/vial 100ug $156.00/vial when ordered with antibody

Also available: intact

non conjugated cat#RDI-CD11cabm-39 $312.00/100ug

FITC conjugated: cat#RDI-CD11cabm-39FT $375.00/120T

Biotin conjugated: cat#RDI-CD11cabm-39BT $375.00/100ug

PE conjugated: cat#RDI-CD11cbm-39PE $500.00/100T

Bulk quotes on request


Mouse anti-human CD11C:Biotin clone 3.9

cat# RDI-CD11Cabm-39BT    $375.00

Presentation: 100ug in 0.1ml (1mg/ml) 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. 5% glycerol, 0.2% BSA and 0.04% sodium azide.

Clone: 3.9

Isotype: Mouse IgG1

Immunogen: Human rheumatoid synovial fluid cells/monocytes.

PRODUCTION: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. culture supernatant and conjugated to NHS-Biotin. Unconjugated Biotin separated by desalting column.

PERFORMANCE: Five x 105 ficoll prepared human peripheral blood lymphocytes were washed and incubated 45 minutes on ice with 80 ul of anti-CD11c:Biotin at 5ug/ml .Cells were washed twice and incubated with 2o reagent Streptavidin:PE, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.57 log10 fluorescent units when compared to a Mouse IgG1:Biotin negative control at a similar concentration.

INFORMATION: Human CD11c (alpha X integrin) complexes with CD18 (beta2 integrin) to form the complement receptor type 4 (CR4) heterodimer which binds to fibrinogen and is involved with monocyte/granulocyte adhesion during inflammatory responses. CD11c expression is restricted to leukocytes mainly of myeloid lineage with highest expression on macrophages. Antibody 3.9 recognizes the integrin alpha X subunit (CD11c) of about 150 kd.

References: N. Hogg,et al, (1986) Eur J Immunol 16: 240-248. S.A. Stacker & T.A. Springer, (1991) J Immunol 146: 648-655. M. Patarroyo, (1994) Immunobiology 191: 474-477. Leukocyte Typing V (1995) (S.F. Schlossman, et al., eds.) Oxford University Press, Oxford, p. 1590-1592.

For Research Use Only

Recommended BIOTIN Isotype Control

cat#RDI-MIGG1BTX2   $312.00/vial 100ug


Anti-human CD11c

PRODUCT CODE RDI-CBL522    $375.00/vial 200ug

    also available: PE labeled cat#RDI-CBL522PE    $469.0/100 Test vial


CLONE: HC1/1

ISOTYPE: IgG1

SOURCE: tissue culture supernatant

Immunizing Agent: Monocytic cell line U937 differentiated with phorbol esters

Fusion Partner: SP2

PURIFICATION Protein A affinity chromatography


SPECIFICITY: This antibody reacts with the alpha X chain of the CD11c/CD18 (p150/95) integrin heterodimer expressed monocytes and weakly on granulocytes. The antibody stains macrophages in most types of tissues and in flow cytometry labels a T cell subset and weakly a B cell subset. Expression is up regulated by inflammatory mediators. The CD11c antigen is a marker for hairy cell leukaemia. When the 11c antigen is a marker for hairy cell leukaemia. When the 11c antigen is complexed with CD18, it is known as the leucocyte function antigen-1 (LFA-1).

Antigen distrbution: Monocytes >23%

                              Granulocytes 25%

                              Peripheral blood lymphocytes <3%


APPLICATIONS:

*Flow cytometric studies to detect LFA deficiency

*characterization of leukaemias and lymphomas in either flow cytometry or on frozen sections

*Detection of macrophages in frozen tissue

*Immunoprecipitation studies of the CD11C moiety

*Studies on adhesion blockade of the CD11c antigen


References: Leucocyte Typing V, Oxford University Press (1995)

                   Cabanas, C. et al, Hybridoma 7, 167-175 (1988)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in in phosphate buffred saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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