rev:   February 15, 2005

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD105 Antibodies

Mouse anti-human CD105 antibodies

-3 clones    & 1 polyclonal

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CBL418                  8E11                             V             mIgM       purified    $375.00

RDI-CBL418FT                " "                               "                 "            FITC        $375.00

RDI-CD105-266             266                              --               IgG1       purified    $438.00

RDI-CD105-SN              SN6                                               IgG1k     purified    $312.00

RDI-CD105-SNPE            "                                                 IgG1        PE            $469.00

                                 (clone 8E11 is a Cytolytic complement fixing clone)

RDI-102PA60              rabbit polyclonal  

CD105

PRODUCT CODE :RDI- CBL 418     $375.00/vial of 200ug purified

   Also available FITC labeled, order cat#RDI-CBL418FT $375.00/100T

CLONE         8E11

ISOTYPE       IgM

IMMUNIZING ANTIGEN       PMA-induced U-937 cells

FUSION PARTNER                 NS1 myeloma cell line

SOURCE Tissue culture supernatant

PURIFICATION METHOD         Ammonium sulphate precipitation followed by gel filtration

SPECIFICITY Endoglin is an endothelial homodimeric membrane antigen containing the tripeptide sequence RGD, which is a recognition motif for adhesion receptors of the integrin family. It has been proposed that endoglin is a TGF-b receptor. Endoglin is a glycoprotein present on endothelial cells of capillaries, arterioles and venules in a variety of tissues and at low levels on acute lymphoblastic and myelocytic leukaemia cells

APPLICATIONS   *Study of monocyte differentiation into macrophages and endoglin up-regulation*Studies in cellular adhesion of circulating blood cells*Lysis of CD105+ cells in the presence of complement*Identification of CD105 positive cells on formalin fixed paraffin wax embedded tissue sections

REFERENCES Lastres, P. et al. Eur. J. Immunol 22, 393-397 (1992)Lopez-Casillas, F. et al. Cell 67, 785-95 (1991)O'Connell, P. J. et al. Clin. Exp. Immunol. 90, 154-59 (1992)Fernandez-Ruiz, E. et al. Cytogonet. Cell Ganet. 64, 204-207 (1993)Bellon, T. et al. Eur. J. Immunol. 23, 2340-45 (1993)Lastres, P. et. al. Biochem. J. 301, 765-68 (1994)Westenend, P.J. et al. J. Am. Soc. Neph. 5, 822 (1994)

LOT SPECIFIC DATA

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at +4oC or add an equal volume of glycerol and store at -20oC.

also available:Fitc labeled cat#RDI-CBL418FT $375.00/100T vial

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures

Catalog#: RDI-CD105-266     $438.00/vial    

Package Size: 100ug purified in 0.2ml (0.5mg/ml) 10mMPBS (pH7.2), 150mM NaCl and 0.09% sodium azide. (sodium azide is toxic,dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)

Prep: purified from tissue culture supernatant via Protein G affinity chromatography.

Species: mouse IgG1k

CLONE: 266

Workshop Code: not applicable

Activity: Reacts with a homodimeric integral membrane protein comprised of 95Kd subunits (CD105). CD105 is a component of the TGF-B receptor system and can bind TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. CD105 is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. U937 cells and activated macrophages also expresses CD105. Expression of CD105 is increased on activated endothelium in tissues undergoing angiogenesis, ,such as in tumors, or in cases of wound healing or dermal inflammation. This antibody is suitable for formalin fixed parrafin embedded tissue sections without pretreatment (using ABC detection, DAB, positive control tissue human thymus).

Application: -Indirect immunofluorescence cell surface staining

- histochemistry (5-10ug/ml)

Titer for each application

References:

1) Int. J. Cancer 54:363

2) J. Biol chem. 267:19027

3) Eur J Immunol. 22:393-397

4) J. Biol Chem. 267:19027-19030

Storage: Store at 4 DEG C.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

cat#RDI-CD105-SN           $312.00/100ug purified

cat#RDI-CD105-SNPE       $469.00/120T PE conjugated

cat#RDI-CD105-SNFT        $375.00/120T FITC conjugated

cat#RDI-CD105-SNBT      $375.00/100ug Biotin conj

-clone SN6 recognizes a CD105 homodimer with a molecular weight of about 160kd. In its purfied format it will augment the binding of TGFB1 to CD105 expressing leukemia cells.

-suitable for flow cytometry, immunoprecipitation, acetone fixed frozen sections

-immunogen:membrane preparation of human B-lineage leukemia cells

-ref:B.K. Seon et al (1996) Tissue Antigens 48:417

Description: Produced from sera of rabbits immunised with highly pure recombinant human soluble CD105/Endoglin produced ed in insect cells. The recombinant soluble CD105/Endoglin consists of amino acid 26 (Glu) to 586 (Leu) and is fused to a C-terminal His-tag (6xHis).

Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70% and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively.

It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.

Host species:     Rabbits

Antigen:             Recombinant human soluble CD105/Endoglin

Purification:       Protein-A Chromatography (+his tag depleted)

Stabilizer:         none

Buffer:               lyophilized from PBS, pH 7.4 w/o preservative

Formulation:      lyophilized rabbit IgG

Reconstitution: The lyophilized IgG is stable at 4°C. for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water/PBS to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C. Avoid repeated freeze-thaw cycles. Reconstituted antibody can also be aliquoted and stored at -20°C to -70°C for at least 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles. -It is recommended that a suitable preservative as 0.09% sodium azide and addition of carrier as 0.1-1% BSA be added to diluted antibody to prevent sticking to sides of vial.

Applications

ELISA: Use at 1-15 µg/ml.

Western Analysis: Use at a concentration of 1-2 µg/ml with the appropriate secondary reagents.

Immunohistochemistry: Not investigated so far.

Optimal dilutions should be determined by each laboratory for each application.

Usage: Anti-human CD105/Endoglin is offered for research use. Not for drug use. Not for human use.

Catalogue number: 102-PA60 Size: 200 µg

Literature: [Cheifetz et al., J Biol Chem 267:19027, 1992; Parker et al., J Bone Miner Res 18:289, 2003; Barbara et al., J Biol Chem 274:584, 1999; Arthur et al., Dev Biol 217:42, 2000; McAllister et al., Nature Genet 8:345, 1994; Fonsatti et al., J Cell Physiol 188:1, 2001].

** please note : always centrifuge vials before opening **

see also recombinant human and mouse CD105 antigen  sCD105,

-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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