rev: June 9, 2005
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CD CLUSTERED
ANTIBODIES
(anti-Human and others as indicated)
NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).
Research Diagnostics Inc offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD102 (ICAM-2)
Antibodies
Mouse anti-human CD102 antibodies
-2 clones
CATALOG# CLONE# Wokshop Host Form Price
RDI-ICAM2 CBR-IC2/2 V mIgG2a purified $400.00
RDI-CBL539 BT-1 -- mIgG1 puirified $495.00
RDI-CBL539FT " --- " FITC $495.00
polyclonals
DATA SHEET: mouse anti-human ICAM-2
Catalog#: RDI-ICAM2 $400.00/vial
Batch#
Package Size: 50 micrograms in 1ml PBS with 1% BSA and 0.01% thimerosal
Species: mouse IgG2a (>95% by SDS-gel).
CLONE: CBR-IC2/2
Activity: The anti-ICAM2 specifically reacts with Intracellular Adhesion
Molecule-2, a 60 kDa surface glycoprotein that promotes adhesion between
immune cells and the vascular endothelium. This molecule is a member of the
Ig superfamily with two immunoglobulin like extracellular domains. Adhesion
events play a central role in the function of the immune system in directing
cell-cell interactions critical for antigen-presentation, lymphocyte activation,
migration and effector/target function at the site of inflammation.
-ICAM 2 is broadly distributed on hematopoietic cells. On resting lymphocytes,
the ICAM-2 expression is several fold higher than that seen with ICAM-1,
while monocytes express equivalent levels of ICAM-1 and ICAM- 2. In contrast
to ICAM-1, neutrophils stain negative for ICAM-2. Just little o no ICAM-2
staining can be demonstrated on other cell lines, the sole exception being
a) resting vascular endothelial cells, which possess high levels of ICAM-2
and b) small clusters of cells in lymphoid tissue germinal centers. -ICAM-2
expression is unaffected by a variety of inflammatory cytokines, while ICAM-1
is upregulated upon stimulation. This points towards the importance of ICAM-2
in the unstimulated resting state, before ICAM-1 expression is increased.
-The integrin LFA-1 is the receptor for ICAM- 2 and ICAm-1. The functional
characteristics of ICAM-2 as an LFA-1 ligand are as follows: Icam-2 dominates
over ICAM-1 on resting endothelial cells and seems to be involved in the
recirculation of LFA-1 positive lymphocytes, e.g., facilitating T-memory
cell recirculation. As resting T-cells express little or no ICAM-1, ICAM-2
may also be important in initial T-cell adhesion with antigen-presenting
cells that bear LFA-1. Also the lysis of certain target cells appear to occur
in an ICAM-1 independent manner, possibly regulated by ICAM-2 (1,2).
Application: Anti-ICAM-2 can be used to stain acetone fixed cryostat sections
or cell smears. The initial dilution is 1:50 to 1:200 (optimal dilution should
be determined by the individual lab for each application.
-also suitable for indirect flow cytometry.
Storage: Store at 2-8 DEG C.
ref: J. Exp. Med, 174, 253-267.
Eur J. Biochem, 195, 177-182.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. Weare
not responsible for any patent infringements that might result with the use
of or derivation of this product.
PRODUCT CODE :RDI-CBL539 $495.00/vial 100ug
-also availalel FITC labeled cat#RDI-CBL539FT $495.00/100T
CLONE: B-T1
ISOTYPE: IgG1
SOURCE: mouse ascitic fluid
Immunizing Antigen: ICAM-2 transfected CHO cell line
Fusion Partner: Balb/c (Iffa Credo/ X63/AG.8653)
PURIFICATION METHOD: Ion exchange chromatography.
SPECIFICITY: Recognizes a 55-56kDa protein
APPLICATIONS: * Flow cytometry
REFERENCES: Vermont-Desroches et al. Eur. J. Immunol 22, 2460-2464 (1995)
Jinglin XIE et al, J. Immunol. 22, 1767-1795 (1995)
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration
of 100ug in 1ml phosphate buffered saline containing 10mM sodium azide
and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine
an optimum working titre for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods
at 4oC. However repeated warming to room temperature and re-cooling may result
in a loss of activity and hence effective working titre. It is recommended
that monoclonal antibodies are aliquotted upon receipt and then stored at
4 DEG C. Dilute working solutions are best prepared on the day of use. DO
NOT fREEZE CONJUGATED ANTIBODY. Protect from Light.
For research use only. Not supplied for use in human diagnostic or therapeutic
procedures
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446
fax (978) 371-2266
email: antibodies@fitgzerald-fii.com
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