rev:   June 9, 2005

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD102 (ICAM-2) Antibodies


Mouse anti-human CD102  antibodies

-2 clones  


CATALOG#                 CLONE#                  Wokshop    Host         Form        Price

RDI-ICAM2                  CBR-IC2/2                     V            mIgG2a     purified     $500.00

RDI-CBL539                    BT-1                            --            mIgG1       puirified    $495.00

RDI-CBL539FT                  "                                 ---               "           FITC        $495.00

polyclonals


DATA SHEET: mouse anti-human ICAM-2

Catalog#: RDI-ICAM2       $500.00/vial  

Batch#

Package Size: 50 micrograms in 1ml PBS with 1% BSA and 0.01% thimerosal

Species: mouse IgG2a (>95% by SDS-gel).

CLONE: CBR-IC2/2

Activity: The anti-ICAM2 specifically reacts with Intracellular Adhesion Molecule-2, a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. This molecule is a member of the Ig superfamily with two immunoglobulin like extracellular domains. Adhesion events play a central role in the function of the immune system in directing cell-cell interactions critical for antigen-presentation, lymphocyte activation, migration and effector/target function at the site of inflammation.

-ICAM 2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM- 2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little o no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being a) resting vascular endothelial cells, which possess high levels of ICAM-2 and b) small clusters of cells in lymphoid tissue germinal centers. -ICAM-2 expression is unaffected by a variety of inflammatory cytokines, while ICAM-1 is upregulated upon stimulation. This points towards the importance of ICAM-2 in the unstimulated resting state, before ICAM-1 expression is increased.


-The integrin LFA-1 is the receptor for ICAM- 2 and ICAm-1. The functional characteristics of ICAM-2 as an LFA-1 ligand are as follows: Icam-2 dominates over ICAM-1 on resting endothelial cells and seems to be involved in the recirculation of LFA-1 positive lymphocytes, e.g., facilitating T-memory cell recirculation. As resting T-cells express little or no ICAM-1, ICAM-2 may also be important in initial T-cell adhesion with antigen-presenting cells that bear LFA-1. Also the lysis of certain target cells appear to occur in an ICAM-1 independent manner, possibly regulated by ICAM-2 (1,2).

Application: Anti-ICAM-2 can be used to stain acetone fixed cryostat sections or cell smears. The initial dilution is 1:50 to 1:200 (optimal dilution should be determined by the individual lab for each application.

-also suitable for indirect flow cytometry.

Storage: Store at 2-8 DEG C.

ref: J. Exp. Med, 174, 253-267.

Eur J. Biochem, 195, 177-182.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Weare not responsible for any patent infringements that might result with the use of or derivation of this product.


ANTI-HUMAN CD102 (ICAM-2) ANTIGEN


PRODUCT CODE :RDI-CBL539  $495.00/vial  100ug

-also availalel FITC labeled  cat#RDI-CBL539FT  $495.00/100T


CLONE: B-T1


ISOTYPE: IgG1


SOURCE: mouse ascitic fluid

Immunizing Antigen: ICAM-2 transfected CHO cell line


Fusion Partner: Balb/c (Iffa Credo/ X63/AG.8653)

PURIFICATION METHOD: Ion exchange chromatography.


SPECIFICITY: Recognizes a 55-56kDa protein


APPLICATIONS: * Flow cytometry


REFERENCES: Vermont-Desroches et al. Eur. J. Immunol 22, 2460-2464 (1995)

                           Jinglin XIE et al, J. Immunol. 22, 1767-1795 (1995)


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of  100ug in 1ml phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at 4 DEG C. Dilute working solutions are best prepared on the day of use. DO NOT fREEZE CONJUGATED ANTIBODY. Protect from Light.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Div of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

phone (978) 371-6446

fax     (978) 371-2266

email: antibodies@fitgzerald-fii.com

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