rev: July 14, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-TUBULAAbm-DM $438.00/100ug
RDI-TUBULAabm-DM1
$688.00/200ug
Presentation: purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM
sodium azide (200ug/ml)
-also available azide/BSA free cat#RDI-TUBULAabm-DMX $750.00/200ug
Clone ref: DM1A
Isotype: mIgG1a
Immunogen: Native chick brain microtubules
Hybridoma: fusion between BALB/c57 splenocytes and myeloma NS-1 cells.
Species Reactivity: Human, Cow, Pig, Rat,Mouse, Guinea pig, Gerbil, Frog, Chicken, Yeast, and Fungi.
Others-not tested.
Use: -western blot (1ug/ml for 2 hours RT)
-histochemsitry: frozen sections and formalin fixed paraffin sections (no special pretreatment required)
-electron microscopy &
immunofluorescence
Positive Control: LS174T, MAD109 cells. Skin or lung
Localization: cytoplasmic
Specificity: Recognizes a protein of 57kDa, identified as tubulin-alpha.
This Mab is highly specific to alpha tubulin and shows no cross-reaction
with Beta tubulin . Its epitope localizes between aa 426-450; aa 426-430
are critical for reactivity.2 . This Mab causes the 10-nm filaments to collapse
into large lateral aggregates collecting in the cell periphery or tight
juxtanuclear caps. It does not block microtubule assembly. Mabdoes not inhibit
polymerization or depolymerization of platelet tubulin in vitro. It blocks
(by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote
a stable inhibition of adenylyl cyclase.
Key References:
1. Blose SH, et. al. Journal of Cell Biology, 1984, 98(3):847-58.
2. Breitling F, et. al. Journal of Molecular Biology, 1986, 189(2):367-70.
3. Biochim Biophys Acta, 1989, 1012(1):46-56.
4. Biochemistry, 1993, 32(18):4955-61.
For Research Use Only
Catalog#: RDI-TUBULabSX $625.00/1mg $500.00/mg 5+
Amount: 1 mg (lyophilized), Reconstitute with 1 ml di-H20 or PBS for a 1
mg/ml solution.
Preservative: None
Storage: 2 - 8 C, Stable for 6 months after reconstitution.
Production: The tubulin antigen was isolated from bovine brain origin. The
antiserum was produced in sheep by repeated immunizations with the tubulin
antigen. The antiserum was affinity purified over 6S tubulin Sepharose. Each
vial contains 1 mg IgG + 2 mg bovine serum albumin.
Performance: Useful in the immunohistochemical and immunofluorescent detection of alpha and beta tubulin in tissue sections and cultured cells. Reacts with both interphase and mutotic tubulins and with soluble monomers (6S). The antibody cross reacts with tubulin is human, monkey, hamster and rat. Inhibits tubulin polymerization (assembly) in vitro. A dilution 1/10 is suggested for experiments.
For Research Use Only
Catalog#: RDI-tubulinabm $438.00
Larger sizes quoted on request
Package Size: lyophilized, reconstitute to 0.1ml with sterile PBS buffer
and 0.1%NaN3,
lot#
Ig Isotype: mouse IgM (ascites)
Antigen: Calf Brain tubulin, highly purified
Dilution: approx 1:20-1:100 (optimize for each use)
Reactivity: reacts with human, mouse, rat and hamster tubulin. In immunoblotting,
recognizes only tubulin. On immunofluorescence, reacts with both alpha and
beta microtubules but not with microfilaments or intermediate
filaments.
Storage: Store at -20 DEG C Upon receipt Avoid repeated freeze thaw
cycles.
Ref# Cell 12:561, 1977
Sample Staining Protocol:
1. Prepare fresh DTSP (dithiobis succinimidyl propionate; from Pierce Chemical
Co.) Weigh 5mg, add 0.25ml DMSO and just before use add 24.75 ml warm PBS.
2. Cell grown on cover slips are fixed in DTSP 5min/37 DEG C
3. Wash coverslip in PBS, then distilled water
4. Fix in acetone (-20 DEGC) for 2-5 min
5. Wash in water, then PBS
6. Incubate with 2% BSA for 15min/37 DEG C.
7. Incubate with tubulin antibodies for 60min/37 DEG C.
8. Wash in PBS
9. Incubate with rhodamine-conjugated goat anti-mouse IgM for 60 min 37 DEg
C.
10. Wash in PBS, then water before mouunting slide.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. Sodium azide may form explosive compounds
in presence of heavy metals or under acidic conditions. Flush drains with
copious amounts of water to prevent buildup of explosive compounds.
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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