rev: May 13, 2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-Tubulin (see also antibodies for beta tubulin, and beta III isoform and gamma)
Mouse anti-Alpha Tubulin (Multi-Species) Paraffin Sections/Western Blot
cat# RDI-TUBULAAbm-DM, new cat#: 10R-T130ax $484.00/100ug
RDI-TUBULAabm-DM1, new cat#: 10R-T130a
$724.50/200ug
Presentation: purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM sodium
azide (200ug/ml)
-also available azide/BSA free cat#RDI-TUBULAabm-DMX $750.00/200ug
Clone ref: DM1A
Isotype: mIgG1a
Immunogen: Native chick brain microtubules
Hybridoma: fusion between BALB/c57 splenocytes and myeloma NS-1 cells.
Species Reactivity: Human, Cow, Pig, Rat,
Others-not tested.
Use: -western blot (1ug/ml for 2 hours RT)
-histochemsitry: frozen sections and formalin fixed paraffin sections (no special pretreatment required)
-electron microscopy
& immunofluorescence
Positive Control: LS174T, MAD109 cells. Skin or lung
Localization: cytoplasmic
Specificity: Recognizes a protein of 57kDa, identified as tubulin-alpha. This
Mab is highly specific to alpha tubulin and shows no cross-reaction with Beta tubulin . Its epitope localizes between aa 426-450; aa
426-430 are critical for reactivity.2 . This Mab
causes the 10-nm filaments to collapse into large lateral aggregates collecting
in the cell periphery or tight juxtanuclear caps. It does not block microtubule
assembly. Mabdoes not inhibit polymerization or depolymerization of platelet
tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with
GppNHp bound) to promote a stable inhibition of adenylyl cyclase.
Key References:
1. Blose SH, et. al. Journal of Cell Biology, 1984, 98(3):847-58.
2. Breitling F, et. al. Journal of Molecular Biology, 1986, 189(2):367-70.
3. Biochim Biophys Acta, 1989, 1012(1):46-56.
4. Biochemistry, 1993, 32(18):4955-61.
For Research Use Only
Sheep anti-Tubulin (affinity purified)
Catalog#: RDI-TUBULabSX $625.00/1mg $500.00/mg 5+
Amount: 1 mg (lyophilized), Reconstitute with 1 ml di-H20 or PBS for a 1
mg/ml solution.
Preservative: None
Storage: 2 - 8 C, Stable for 6 months after reconstitution.
Production: The tubulin antigen was isolated from bovine brain origin. The
antiserum was produced in sheep by repeated immunizations with the tubulin
antigen. The antiserum was affinity purified over 6S tubulin Sepharose. Each
vial contains 1 mg IgG + 2 mg bovine serum albumin.
Performance: Useful in the immunohistochemical and immunofluorescent detection of alpha and beta tubulin in tissue sections and cultured cells. Reacts with both interphase and mutotic tubulins and with soluble monomers (6S). The antibody cross reacts with tubulin is human, monkey, hamster and rat. Inhibits tubulin polymerization (assembly) in vitro. A dilution 1/10 is suggested for experiments.
For Research Use Only
DATA SHEET: (mouse anti-Tubulin A & B)
Catalog#: RDI-tubulinabm, new cat#: 10R-T129a $484.00
Package Size: lyophilized, reconstitute to 0.1ml with sterile PBS buffer and 0.1%NaN3,
lot#
Ig Isotype: mouse IgM (ascites)
Antigen: Calf Brain tubulin, highly purified
Dilution: approx 1:20-1:100 (optimize for each use)
Reactivity: reacts with human, mouse, rat and hamster tubulin. In immunoblotting, recognizes only tubulin. On immunofluorescence, reacts with both alpha and beta microtubules but not with microfilaments or intermediate filaments.
Storage: Store at -20 DEG C Upon receipt Avoid repeated freeze thaw cycles.
Ref# Cell 12:561, 1977
Sample Staining Protocol:
1. Prepare fresh DTSP (dithiobis succinimidyl propionate; from Pierce Chemical
Co.) Weigh 5mg, add 0.25ml DMSO and just before use add 24.75 ml warm PBS.
2. Cell grown on cover slips are fixed in DTSP 5min/37
DEG C
3. Wash coverslip in PBS, then distilled water
4. Fix in acetone (-20 DEGC) for 2-5 min
5. Wash in water, then PBS
6. Incubate with 2% BSA for 15min/37 DEG C.
7. Incubate with tubulin antibodies for 60min/37 DEG C.
8.
9. Incubate with rhodamine-conjugated goat anti-mouse IgM for 60 min 37 DEg C.
10. Wash in PBS, then water before mouunting slide.
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. Sodium azide may form explosive compounds in
presence of heavy metals or under acidic conditions. Flush drains with copious
amounts of water to prevent buildup of explosive compounds.
band approx 52-54kda with HElA, NIH/3T3 or A-431 whole cell lysates
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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