rev:   June 3, 2004

HOME (index page)

Return (alphabetical antibody page)

Return  (cytokeratin data page)


ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Beta Tubulin


Mouse anti-Beta Tubulin (Multi-Species)   Paraffin Sections/Western Blot

cat# RDI-TUBULBAbm-5      $438.00/100ug

       RDI-TUBULBabm-51    $688.00/200ug

Presentation: purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM sodium azide (200ug/ml) -also available azide/BSA free cat#RDI-TUBULBabm-5X $750.00/200ug

Clone ref: TBN05 (also known as TUb 2.1)

Isotype: mIgG1

Immunogen: purified tubulin

Epitope: C-terminal

Species Reactivity: Human, Cow, Rabbit,Mouse, Chicken, Rat, Frog, Moth, and Wheat. Others not known.

Localization: Cytoplasmic

Use: -western blot (0.5ug/ml for 2 hours RT)

-histochemistry: frozen sections and formalin fixed paraffin sections (no special pretreatment required) 1-2ug/ml

-electron microscopy & immunofluorescence

Positive Control: Skin or lung, MAD109cells or Ls174T

Specificity: Recognizes a protein of 55kDa, identified as tubulin Beta.

Key References: 1. Gozes, I., and Barnstable, C.J., Proc. Natl. Acad. Sci. USA, 79, 2579 (1982).

                            2. Matthes, T., et al., J. Immunol., 141, 3135 (1988).

For Research Use Only


Rabbit anti-Beta Tubulin (Multi-Species) Paraffin Sections/Western Blot

cat# RDI-TUBULBabR    $438.00/100ug

        RDI-TUBULBabR1  $688.00/200ug

Presentation: purified rabbit polyclonal antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM sodium azide (200ug/ml)

Immunogen: synthetic peptide derived from aa 416-430 of human tubulin beta

Species Reactivity: Human, Cow, Pig, Rat,Mouse, Guinea pig, Gerbil, Frog, Chicken, Yeast, and Fungi. sea urchin, myxamoeba and plasmodium.Others-not tested.

Localization: Cytoplasmic

Use: -western blot (1ug/ml for 2 hours RT)

       -histochemistry: frozen sections and formalin fixed paraffin sections (no special pretreatment required) 1-2ug/ml

Positive Control: Skin or lung

Specificity: Recognizes a protein of 55kDa, identified as tubulin Beta. Its epitope localizes between aa 416-430. although not tested for this activity, a similar Monoclonal ab clone DM1B (no longer available) did not cause the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.

For Research Use Only




RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

Ordering terms

RETURN(cytokeratin page)