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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-RYANRabm $438.00/vial
Background: The Ryanodine receptor is the channel responsible for the release
of Calcium from the sarcoplasmic reticulum in muscle cells and also plays
a role in Ca2+ regulation in non-muscle cells. The Ryanodine receptor exists
as a homotetramer and is predicted to have a short cytoplasmic C-terminus
and 4-10 transmembrane domains with the remainder of the protein termed the
"foot" region, located in the cytoplasm between the T-tubule and the Sr.
The mammalian RyR is the product of three different genes; RyR- 1(expressed
predominantly in the skeletal muscle and areas of the brain), Ry2 (expressed
predominantly in the heart muscle but also found in the stomach, endothelial
cells and diffuse areas of the brain) and RyR-3 (found in smooth muscle and
the brain (striatum, thalamus and hippocampus).
isotype: mIgG1
clone: C3-33
package: 100ul of diluted ascites fluid in PBS with 0.05%
sodium
antigen: produced from a fusion of P3X63 AG8.653 myeloma cells and spleen
cells from Balb/c mice immunized with canine cardiac ryanodine
receptor.
Specificity: Reacts strongly with RyR-2 and weakly with RyR-1 in a broad
range of species and tissue types. It has been shown to react with RyR in
cardiac muscle of canine, rat, finch and pigeon, visceral smooth muscle of
toad, and in rat brain. It also has shown activity with skeletal muscle of
fish and the beta isoform of frog but only weak reactivity with skeletal
muscle in rabbit and the alpha isoform of frog.
Use:
-Western blot:1ug/ml= recognizes approx 565 kDa band which represents the Ryanodine receptor. In non mammalian vertebrates, a doublet is seen at 565kDa which indicates the A and B isoforms of the receptor.
-Immunohistochemistry: 1ug/ml =strongly stains the myofiber consistent with the SR localization of the RyR.
-immunoprecipitation
REF: -F.A. Lai et al, "Amphibian ryanodine receptors are related to
those of mamallian skeletal or cardiac muscle. American Journal of
Physiology, 263:C365-C373, 1992.
-P.S. McPherson and K.P. Campbell, The
Ryanodine Receptor/Ca2+
Release Channel. The J. Biol. Chem. 268(19):1375-13768, 1993
-J. Junker et al, The J. Biol. Chem, 269(3):1627-1634, 1994
-L. Xu et al, Evidence for a Ca2+
gated ryanodine-sensitive CA2+
release channel in visceral smooth muscle. Proccedings of National
Acadamy Sciences, 91:3294-3298, 1994.
-F.A. Lai et al, Expression of a
cardiac Ca2+ release channel
isoform in mammalian brain. Biochemical Journal, 288:553-564,
1992.
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles.
Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at
1000 RPM to concentrate material in vial.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
Mouse anti-Ryanodine Receptor Monoclonal ab (RyR1 &
RyR2)
cat# RDI-RYANR1abm $438.00/vial
Background: The Ryanodine receptor is the channel responsible for the release
of Calcium from the sarcoplasmic reticulum in muscle cells and also plays
a role in Ca2+ regulation in non-muscle cells. The Ryanodine receptor exists
as a homotetramer and is predicted to have a short cytoplasmic C-terminus
and 4-10 transmembrane domains with the remainder of the protein termed the
"foot" region, located in the cytoplasm between the T-tubule and the Sr.
The mammalian RyR is the product of three different genes; RyR- 1(expressed
predominantly in the skeletal muscle and areas of the brain), Ry2 (expressed
predominantly in the heart muscle but also found in the stomach, endothelial
cells and diffuse areas of the brain) and RyR-3 (found in smooth muscle and
the brain (striatum, thalamus and hippocampus).
isotype: mIgG1
clone: 34-C
package: 100ul of diluted ascites fluid in PBS with 0.05% sodium
antigen: produced from a fusion of P3X63 AG8.653 myeloma cells and spleen
cells from Balb/c mice immunized with partially purified chicken pectoral
muscle ryanodine receptor.
Specificity: Reacts with RyR-1 and RyR-2 isoforms in human, bovine, sheep,
canine, rat, mouse and rabbit. It also recognizes: chicken (the a,b, and
cardiac isoforms), frog (the a and b isoforms), fish (a isoform). In non
mammalian vertebrates, the RyR isoforms have the approx specifiicty as follows
(a=RyR-1, b=RyR-3 and cardiac=RyR- 2).
Use:
-Western blot:1:5000= recognizes approx 565 kDa band which represents the
Ryanodine receptor. In non mammalian vertebrates, a doublet is seen at 565kDa
which indicates the A and B isoforms of the receptor.
-Immunohistochemistry: 1:1000 =strongly stains the myofiber consistent with the SR localization of the RyR.
-immunoprecipitation
REF: -J.A. Airey et al, The Journal of Biological Chemistry, 265(24):14187-14194,
1990.
-P.D. Walton, et al, The Journal of Cell Biology, 113(5),1145-1157, 1995.
-E.B. Olivares, et al, Biophysical Journal,
59:1153-1163, 1995)
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or (800) 370-2222
EMAIL:margaret@cellular-research.com
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