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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


-see 2 clones below
Mouse anti-Ryanodine Receptor Monoclonal ab


cat# RDI-RYANRabm  $438.00/vial


Background: The Ryanodine receptor is the channel responsible for the release of Calcium from the sarcoplasmic reticulum in muscle cells and also plays a role in Ca2+ regulation in non-muscle cells. The Ryanodine receptor exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains with the remainder of the protein termed the "foot" region, located in the cytoplasm between the T-tubule and the Sr. The mammalian RyR is the product of three different genes; RyR- 1(expressed predominantly in the skeletal muscle and areas of the brain), Ry2 (expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain) and RyR-3 (found in smooth muscle and the brain (striatum, thalamus and hippocampus).

isotype: mIgG1

clone: C3-33

package: 100ul of diluted ascites fluid in PBS with 0.05% sodium

antigen: produced from a fusion of P3X63 AG8.653 myeloma cells and spleen cells from Balb/c mice immunized with canine cardiac ryanodine receptor.

Specificity: Reacts strongly with RyR-2 and weakly with RyR-1 in a broad range of species and tissue types. It has been shown to react with RyR in cardiac muscle of canine, rat, finch and pigeon, visceral smooth muscle of toad, and in rat brain. It also has shown activity with skeletal muscle of fish and the beta isoform of frog but only weak reactivity with skeletal muscle in rabbit and the alpha isoform of frog.

Use:

-Western blot:1ug/ml= recognizes approx 565 kDa band which represents the Ryanodine receptor. In non mammalian vertebrates, a doublet is seen at 565kDa which indicates the A and B isoforms of the receptor.

-Immunohistochemistry: 1ug/ml =strongly stains the myofiber consistent with the SR localization of the RyR.

-immunoprecipitation

REF: -F.A. Lai et al, "Amphibian ryanodine receptors are related to

          those of mamallian skeletal or cardiac muscle. American Journal of

          Physiology, 263:C365-C373, 1992.


          -P.S. McPherson and K.P. Campbell, The Ryanodine Receptor/Ca2+

           Release Channel. The J. Biol. Chem. 268(19):1375-13768, 1993

           -J. Junker et al, The J. Biol. Chem, 269(3):1627-1634, 1994


           -L. Xu et al, Evidence for a Ca2+ gated ryanodine-sensitive CA2+

             release channel in visceral smooth muscle. Proccedings of National

             Acadamy Sciences, 91:3294-3298, 1994.


           -F.A. Lai et al, Expression of a cardiac Ca2+ release channel

             isoform in mammalian brain. Biochemical Journal, 288:553-564,

             1992.



Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-Ryanodine Receptor Monoclonal ab (RyR1 & RyR2)

cat# RDI-RYANR1abm     $438.00/vial


Background: The Ryanodine receptor is the channel responsible for the release of Calcium from the sarcoplasmic reticulum in muscle cells and also plays a role in Ca2+ regulation in non-muscle cells. The Ryanodine receptor exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains with the remainder of the protein termed the "foot" region, located in the cytoplasm between the T-tubule and the Sr. The mammalian RyR is the product of three different genes; RyR- 1(expressed predominantly in the skeletal muscle and areas of the brain), Ry2 (expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain) and RyR-3 (found in smooth muscle and the brain (striatum, thalamus and hippocampus).


isotype: mIgG1

clone: 34-C

package: 100ul of diluted ascites fluid in PBS with 0.05% sodium

antigen: produced from a fusion of P3X63 AG8.653 myeloma cells and spleen cells from Balb/c mice immunized with partially purified chicken pectoral muscle ryanodine receptor.

Specificity: Reacts with RyR-1 and RyR-2 isoforms in human, bovine, sheep, canine, rat, mouse and rabbit. It also recognizes: chicken (the a,b, and cardiac isoforms), frog (the a and b isoforms), fish (a isoform). In non mammalian vertebrates, the RyR isoforms have the approx specifiicty as follows (a=RyR-1, b=RyR-3 and cardiac=RyR- 2).

Use:
-Western blot:1:5000= recognizes approx 565 kDa band which represents the Ryanodine receptor. In non mammalian vertebrates, a doublet is seen at 565kDa which indicates the A and B isoforms of the receptor.

-Immunohistochemistry: 1:1000 =strongly stains the   myofiber consistent with the SR localization of the RyR.

-immunoprecipitation



REF: -J.A. Airey et al, The Journal of Biological Chemistry, 265(24):14187-14194, 1990.

         -P.D. Walton, et al, The Journal of Cell Biology, 113(5),1145-1157, 1995.

          -E.B. Olivares, et al, Biophysical Journal, 59:1153-1163, 1995)

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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