rev: 5/15/2008
HOME (index page)
Return (alphabetical antibody page)
Return (ion transport data page)
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see below 6abs, one each to alpha-1, alpha-2, alpha -3, beta -1, beta -2 & beta -3
BATCH SHEET: Rabbit anti-RAT NA-K-ATPASE, alpha-1
Catalog#: RDI-RTNKATPabr, new cat#: 20R-NR002
Price: $624.75/150ug
Packaging: 150 micrograms in 0.1M Tris-glycine pH 7.4
with .05% NaN3. Purified by Protein G.
Species: rabbit IgG
Antigen: peptide sequence corresponding to amino acids 338-518 deduced from the
cDNA for rat NA-K ATPase
alpha 1 subunit
Specificity: specific for isoform for RAT Na-K Atpase alpha 1 subunit, cross reactivity with other species
not determined
Uses: Western blot: 1-0.1ug/ml dilution in blocking solution (TRis buffered saline containing 5% non fat dry milk and
0.5% Tween 20); detects Na-K ATPase
alpha 1 isoform in 50 micrograms rat brain and kidney
microsomal protein using the ECL system. -Do
not boil microsomes (warm to 37 DEG C)
- IMMUNOPRECIPITATION: 2ug ab /20ug rat brain or kidney microsomal prep
- Histochemistry: 5ug/ml in kidney or brain frozen sections that were fixed for 30 minutes with 4% PFA.
ref: Biochemistry 28: 4531, 1989
Storage: Store at -20 DEG C
AVOID FREQUENT FREEZE THAW CYCLES.
BLOTTING CONTROL: cat#RDI-RTctrl-144 $312.00/400 micrograms
from rat brain microsomal prep: suitable for 10 western blots
active for alpha-1,2,3 & beta-1 and beta 2 Na K ATPase
BLOTTING CONTROL: cat#RDI-RTctrl-145 $312.00/400 micrograms
from rat heart microsomal prep: suitable for 10 western blots
active for alpha-1,2, & beta-1 Na K ATPase
BLOTTING CONTROL: cat#RDI-RTctrl-146 $312.00/400 micrograms
from rat kidney microsomal prep: suitable for 10 western blots
active for alpha-1 & beta-1 Na K ATPase
Rabbit
anti-RAT NA-K-ATPASE, alpha-2
Catalog#: RDI-RT2NKATPabr -sold out discontinued
Rabbit anti-RAT NA-K-ATPASE, alpha-3
Catalog#: RDI-RT3NKATPabr, new cat#: 20R-NR003 Price: $624.75/125ug
Packaging: 125 micrograms IgG in 107ul Tris buffered saline, 0.15M NaCl
containing 30mM Tris-HCL, pH 7.5) with 0.02% sodium azide
Species: purified rabbit IgG by Protein A
chromatography
Antigen: peptide sequence corresponding to amino acids 320-514 deduced from the
cDNA for rat NA-K ATPase
alpha 3 subunit
Specificity: specific for isoform for RAT Na-K Atpase alpha 3 subunit, cross reactivity with other species
not determined
Uses: Western blot: 1ug/ml dilution in blocking solution (TRis
buffered saline containing 5% non fat dry milk and 0.5% Tween
20); detects Na-K ATPase alpha 1 isoform
in 50 micrograms rat brain microsomal protein using
the ECL detection system.
-DO NOT BOIL Microsomes. Recommend 37 DEG C).
IMMUNOCYTOCHEMISTRY: 1:200 in TBS
ref: Biochemistry 28: 4531, 1989
Storage: Store at -20 DEG C
AVOID FREQUENT FREEZE THAW CYCLES.
Rabbit anti-RAT
NA-K-ATPASE, BETA-1
Catalog#: RDI-RTB1NKATPabr Price: $594.00
Packaging: 200 microliters neat serum (recommend
diluting in Tris buffered saline, 0.15M NaCl containing 30mM Tris-HCL, pH
7.5
Species: rabbit IgG
Antigen: peptide sequence corresponding to amino acids 152-340 deduced from the
cDNA for rat NA-K ATPase
beta 1 subunit
Specificity: specific for isoform for RAT Na-K ATPase beta 1 subunit, cross reactivity with other species
not determined
Uses: Western blot: 1:1000 dilution in blocking solution (TRis
buffered saline containing 5% non fat dry milk and 0.5% Tween
20); detects Na-K ATPase alpha 1 isoform
in 40 micrograms rat microsomal protein using the ECL
detection system.
-DO NOT BIOL microsomes. Recommend 37 DEG C)
IMMUNOCYTOCHEMISTRY: 1:200 in TBS
ref: Biochemistry 28: 4531, 1989
Storage: Store at -20 DEG C
AVOID FREQUENT FREEZE THAW CYCLES.
Rabbit anti-RAT NA-K-ATPASE, BETA 2
Catalog#: RDI-RTB2NKATPabr Price: $594.00
Packaging: 200 microliters neat serum (recommend
diluting in Tris buffered saline, 0.15M NaCl containing 30mM Tris-HCL, pH
7.5
Species: rabbit IgG
Antigen: peptide sequence corresponding to amino acids 63-285 deduced from the cDNA for rat NA-K ATPase beta 2
subunit
Specificity: specific for isoform for RAT Na-K Atpase beta 2, cross reactivity with other species not
determined the beta 2 subunit is apparently identical to the glial cell adhesion molecule (a non specific band at 65kD
may be detected).
Uses: Western blot: 1:1000 dilution in blocking solution (TRis
buffered saline containing 5% non fat dry milk and 0.5% Tween
20); detects Na-K ATPase alpha 1 isoform
in 40 micrograms rat microsomal protein using the ECL
detection system.
-DO NOT BOIL Microsomes. Recommend 37 DEG C.
IMMUNOCYTOCHEMISTRY: 1:200 in TBS
ref: Biochemistry 28: 4531, 1989
Storage: Store at -20 DEG C
AVOID FREQUENT FREEZE THAW CYCLES.
BATCH SHEET: Rabbit anti-RAT NA-K-ATPASE, BETA 3
Catalog#: RDI-RTB3NKATPabr
Price: $594.00
Packaging: 200 ug purified ab
in Tris buffered saline, 0.15M NaCl containing 30mM Tris-HCL, pH
7.5
Species: rabbit IgG
Antigen: peptide sequence corresponding to amino acids 70-279 deduced from
the cDNA for rat NA-K ATPase
beta 3 subunit
Specificity: specific for isoform for RAT Na-K Atpase beta 3, cross reactivity with other species not
determined
Uses: Western blot: 0.5-1ug/ml dilution in blocking solution (TRis buffered saline containing 5% non fat dry milk and
0.5% Tween 20); detects Na-K ATPase
beta 3 isoform in 40 micrograms rat brain microsomal protein using the ECL detection system. -DO NOT
BOIL Microsomes. Recommend 37 DEG C.
-To reduce background, recommend washing blot with several changes of buffer containng 0.1% Tween-20 and then a final wash with a buffer containing 0.5M NaCl.
IMMUNOCYTOCHEMISTRY:
-Immunoprecipitation:4ug
general ref: Mamm Genome 9:136-143, 1998
Storage: Store at -20 DEG C
AVOID FREQUENT FREEZE THAW CYCLES.
BLOTTING CONTROL: cat#RDI-RTctrl-144 $312.00/400 micrograms
from rat brain microsoaml prep: suitable for 10 western blots
active for alpha-1,2,3 & beta-1 and beta 2 & beta 3 Na K ATPase
BLOTTING CONTROL: cat#RDI-RTctrl-145 $312.00/400 micrograms
from rat heart microsomal prep: suitable for 10 western blots
active for alpha-1,2, & beta-1 Na K ATPase
BLOTTING CONTROL: cat#RDI-RTctrl-146 $312.00/400 micrograms
from rat kidney microsomal prep: suitable for 10 western blots
active for alpha-1 & beta-1 Na K ATPase
FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN HUMANS OR ANIMALS
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
RETURN (ion transport data page)