rev:       December 28, 2004

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see below guinea pig (polyclonal) antibodies to: cytokeratin 2E, CK5, CK 8/18, CK9, CK14, CK15, CK17 and pan

(need an anti-guinea pig secondary antibody?)


Guinea Pig Pab to Cytokeratin 2E

Cat#RDI-PROGPCK2E       $469.00

Source                                  Guinea pig (GP HK2e-Am2)

Form                                     Stabilized antiserum

Antigen                                 Synthetic peptide (N-terminal aa 2-23) of human cytokeratin 2e (MW 65,852)

Reactive Polypeptide (Specificity) Basic human cytokeratin 2e (MW 65,852),

Description                       The antiserum represents an excellent marker to study terminal epidermal differentiation. The ab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 1). It is also strongly reactive on hyperkeratosis of diverse origin.

Application                      Suitable for · immunohistochemistry on frozen* and paraffin-embedded** (P) tissue

                                        · cytological material

                                        · immunoblotting*** (Western)

Antigen Recognized              Human

in Species (tested so far)       Bovine: calf hoof epidermis, snooze epithelium; negativewith tongue epithelium;

                                              Mouse: heterogenously on foot sole epidermis only negative with epidermis of other body sites.

Working Dilution                  * 1:200 for immunohistochemistry

                                              ** 1:100 for paraffin-embedded tissue

                                              *** 1:5000 - 1:10 000 for Western blotting (ECL)

Incubation Time                    1 h at RT; extended with P

Storage                                  At 2-8°C

Volume                                 100 µl; contains 0.09% sodium azide and 0.5% BSA

For research use only, not for therapeutic or diagnostic use!

References

Collin C., Moll R., Kubicka S., Ouhayoun J.-P. and Franke W.W. Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation, Exp. Cell Res. 202: 132-141 (1992)..

Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)


Guinea Pig Pab to Cytokeratin 5

Cat#RDI-PROGPCK5            $469.00

Source                                   Guinea pig (GP5.2)

Form                                      Stabilized antiserum

Antigen                                  Recombinant human cytokeratin 5

Reactive Polypeptide (Specificity) Basic cytokeratin 5 (Mr 58 000), expressed in basal and first suprabasal layers of epidermis

Antigen Recognized in           Human, bovine, mouse

Species (tested so far)

Tumors Specifically Detected   The antiserum reacts with cytokeratin 5, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin 5 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references listed below).

Application                          Suitable for· immunohistochemistry on frozen*

                                            and paraffin-embedded** (P) tissue

                                           · cytological material

                                            · immunoblotting*** (Western)

Working Dilution                * 1:100 for immunohistochemistry

                                           ** 1:50 for paraffin-embedded tissue

                                           *** 1:5000 - 1:10 000 for Western blotting (ECL)

Incubation Time                 1 h at RT; extended with P

Storage                              At 2-8°C

Volume                             100 µl; contains 0.09% sodium azide and 0.5% BSA

For research use only, not for therapeutic or diagnostic use!

References

Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).

Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).


Guinea Pig Pab to Cytokeratin 8/18

Cat#RDI-PROGP11   $469.00

Category                                Guinea pig polyclonal (GP11)

 Immunoglobulin                   Class IgG

Form/Purification                  Whole antiserum

Antigen                                   Cytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18

Reactive Polypeptide            Mr 52 500 and 45 500

(Specificity)                           (cytokeratins 8 and 18) of simple type epithelium

Antigen Recognized in         Human, bovine, rat, mouse, Syrian hamster, Xenopus laevis

Tumors Specifically Detected All adeno-Ca tested, undifferentiated CA, hepato-cellular CA, cervix Ca.

Application                          Suitable for

                                            · immunohistochemistry on frozen* and paraffin-embedded**(P) tissue

                                            · cytological material

                                            · immunoblotting*** (Western)

                                           · ELISA

Working Dilution                * 1:100 for immunohistochemistry

                                           ** 1:50 for paraffin-embedded tissue

                                            *** 1:2000 for Western blotting

Incubation Time                  1 h at RT; extended with P

Storage                                At 2-8°C

Volume                               100 µl; contains 0.09% sodium azide

For research use only, not for therapeutic or diagnostic use!

References:

Rüther U. et al.: c-fos Expression interferes with thymus development in transgenic mice. Cell 53, 847-856 (1988).

Bader B.L. et al.: Low level expression of cytokeratins 8, 18 and 19 in vascular smooth muscle cells of human umbilical cord and in cultured cells derived therefrom, with an analysis of the chromosomal locus containing the cytokeratin 19 gene. Eur. J. Cell Biol. 47, 300-319 (1988).

Franke W.W. and Moll R.: Cytoskeletal components of lymphoid organs. Differentiation 36, 145-163 (1987).

Knapp A.C. and Franke W.W.: Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67-79 (1989).

Hacker H.J. et al.: Changes in the cellular phenotype and extracellular matrix during progression of estrogen-induced mesenchymal kidney tumors in Syrian hamsters. Virchows Arch. B Cell Pathol. 60, 213-223 (1991).


Guinea Pig Pab to Cytokeratin 9

Cat#RDI-PROGPCK9        $469.00

Source                                Guinea pig (GPHK9-TY1)

Form                                   Stabilized antiserum

Antigen                               Synthetic peptide of human cytokeratin 9 (corresponding to aa. 450-477), coupled to KLH

Reactive Polypeptide (Specificity) Acidic human cytokeratin 9 (MW 62,129), expressed in the palmoplantar epidermis

Antigen Recognized in          Human, bovine, mouse, rat

Species (tested so far)

Tissues Specifically Detected The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum. (see references listed below).

Application Suitable for       · immunohistochemistry on frozen* and paraffin-

                                              embedded** (P) cells and tissue

                                              · immunoblotting*** (Western)

Working Dilution                 * 1:100-1:200 for immunohistochemistry

                                             ** 1:50 for paraffin-embedded tissue

                                            *** 1:5000 - 1:10 000 for Western blotting (ECL)

Incubation Time                   1 h at RT; extended with P

Storage                                 At 2-8°C

Volume                                100 µl; contains 0.09% sodium azide and 0.5% BSA

For research use only, not for therapeutic or diagnostic use!

References

1. Langbein L et al. Molecular characterization of the body site-specific human cytokeratin 9: cDNA cloning, amino acid sequence, and tissue specificity of gene expression. Differentiation 55:57-71 (1994)

2. Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)


Guinea Pig Pab to Cytokeratin 14

Cat#RDI-PROGPCK14        $470.00

Source                                Guinea pig (GP CK 14.2)

Form                                   Stabilized antiserum

Antigen                               Recombinant human cytokeratin 14

Reactive Polypeptide (Specificity) Acidic cytokeratin14 (Mr 50 000), expressed in basal and

                                    first suprabasal layers of epidermis

Antigen Recognized in        Human, bovine, mouse

Species (tested so far)

Tumors Specifically  Detected The antiserum reacts with cytokeratin 14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin 14 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references listed below).

Application Suitable for     · immunohistochemistry on frozen* and paraffin-

                                            embedded** (P) tissue

                                           · cytological material

                                           · immunoblotting*** (Western)

Working Dilution               * 1:100 - 1:200 for immunohistochemistry

                                           ** 1:50 for paraffin-embedded tissue

                                           *** 1:5000 - 1:10 000 for Western blotting

Incubation Time                 1 h at RT; extended with P

Storage                               At 2-8°C

Volume                               100 µl; contains 0.09% sodium azide and 0.5% BSA

For research use only, not for therapeutic or diagnostic use!

References

Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).

Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).

J Cell Science 113, 2471-2483 (2000)

J cell Biol 161, 1105-1115 (2003)

Eur J Cell Biol 82, 385-400 (2003)


Guinea Pig anti-cytokeratin 15

cat#RDI-PROGPCK15  $469.00/100ul

Source Guinea pig (GP CK 15.1)

Form whole antiserum

Antigen C terminal region (including parts of the rod domain) of recombinant human cytokeratin 1 5

Reactive Polypeptide

(Specificity) MW 50,000 0(pI4.9) intermediate filament polypeptide, cytokeratin 15, detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (eg bronchial epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicule, staining was restricted to the outermost cell layer of the outer root sheath; within the hair follicule several cell layers of the hair follicule were positive with antiserum gp15.1.

In human fetal tissues, cytokeratin 15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with antiserum gp15.1. The antiserum is completely unreactive with cells of liver, colon, urinary bladder, pancreas and lung alveolus.

In tumors, gp15.1 is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy (Heid, Bartek, Leube, Moll, Kaufmann & Franke, unpublished results)

Antigen Recognized in Human

Species (tested so far)

Application Suitable for · immunohistochemistry on frozen sections

· immunoblotting*** (Western)

Working Dilution * 1:100 - 1:200 for immunohistochemistry

                              *** 1:2000 Western blotting (ECL)

Incubation Time 1 h at RT

Storage At 2-8°C

Volume 100 µl; contains 0.09% sodium azide

For research use only, not for therapeutic or diagnostic use!

References

Heiid HW et al, in prep

Jih D.M. et al, J. Cutan Pathol 26, 113-118 (1999)


Guinea Pig Pab to Cytokeratin 17

Cat#RDI-PROGPCK17    $469.00

Form                                 Stabilized antiserum

Antigen                             Recombinant human cytokeratin 17

Reactive Polypeptide (Specificity) Acidic human cytokeratin 17 (Mr 46,000)

Antigen Recognized in       Human

Species (tested so far)

Tissues Specifically Detected The antiserum represents an excellent marker to distinguish myoepithelial cells from luminal epithelium of various glands (mammary, sweat, salivary, bronchial, tracheal, laryngeal, esophageal) and benign from malignant forms of e.g. mammary gland tumors.

Application Suitable for          · immunohistochemistry on frozen* and paraffin-embedded** (P) cells and tissue

                                                · immunoblotting*** (Western)

Working Dilution                    * 1:100-1:200 for immunohistochemistry

                                               ** 1:50 for paraffin-embedded tissue

                                               *** 1:5000 - 1:10 000 for Western blotting (ECL)

Incubation Time                     1 h at RT; extended with P

Storage                                  At 2-8°C

Volume                                 100 µl; contains 0.09% sodium azide and 0.5% BSA

For research use only, not for therapeutic or diagnostic use!

References

Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).

Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).


Guinea Pig Pab to Cytokeratin (PAN)

Cat#RDI-PROGP14     $469.00

Source                              Guinea pig

Form                                 Whole antiserum

Antigen                              Cytoskeletal preparation of bovine snout epidermis

Reactive Polypeptide        Most acidic and basic cytokeratins

(Specificity)                        (Mr 40000 - Mr 68000 polypeptides)

Antigen Recognized in         Human, bovine, Xenopus, rat, mouse

Species (tested so far)

Tumors Specifically Detected    All types of epithelia and epithelium-derived Neoplasms

Application Suitable for            · immunohistochemistry on frozen* and paraffin-

                                                   embedded** (P) tissue

                                                 · cytological material

                                                 · immunoblotting*** (Western)

Working Dilution                    * 1:100 for immunohistochemistry

                                                ** 1:50 for paraffin-embedded tissue

                                                *** 1:2000 for Western blotting

Incubation Time                      1 h at RT; extended with P

Storage                                    At 2-8°C

Volume                                    100 µl; contains 0.09% sodium azide

For research use only, not for therapeutic or diagnostic use!


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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