RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see below guinea pig (polyclonal) antibodies to: cytokeratin 2E, CK5, CK 8/18, CK9, CK14, CK15, CK17 and pan
(need an anti-guinea pig secondary antibody?)
Guinea Pig Pab to Cytokeratin 2E
Cat#RDI-PROGPCK2E $469.00
Source Guinea pig (GP HK2e-Am2)
Form Stabilized antiserum
Antigen Synthetic peptide (N-terminal aa 2-23) of human cytokeratin 2e (MW 65,852)
Reactive Polypeptide (Specificity) Basic human cytokeratin 2e (MW 65,852),
Description The antiserum represents an excellent marker to study terminal epidermal differentiation. The ab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 1). It is also strongly reactive on hyperkeratosis of diverse origin.
Application Suitable for immunohistochemistry on frozen* and paraffin-embedded** (P) tissue
cytological material
immunoblotting*** (Western)
Antigen Recognized Human
in Species (tested so far) Bovine: calf hoof epidermis, snooze epithelium; negativewith tongue epithelium;
Mouse: heterogenously on foot sole epidermis only negative with epidermis of other body sites.
Working Dilution * 1:200 for immunohistochemistry
** 1:100 for paraffin-embedded tissue
*** 1:5000 - 1:10 000 for Western blotting (ECL)
Incubation Time 1 h at RT; extended with P
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide and 0.5% BSA
For research use only, not for therapeutic or diagnostic use!
References
Collin C., Moll R., Kubicka S., Ouhayoun J.-P. and Franke W.W. Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation, Exp. Cell Res. 202: 132-141 (1992)..
Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)
Guinea Pig Pab to Cytokeratin 5
Cat#RDI-PROGPCK5 $469.00
Source Guinea pig (GP5.2)
Form Stabilized antiserum
Antigen Recombinant human cytokeratin 5
Reactive Polypeptide (Specificity) Basic cytokeratin 5 (Mr 58 000), expressed in basal and first suprabasal layers of epidermis
Antigen Recognized in Human, bovine, mouse
Species (tested so far)
Tumors Specifically Detected The antiserum reacts with cytokeratin 5, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin 5 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references listed below).
Application Suitable for immunohistochemistry on frozen*
and paraffin-embedded** (P) tissue
cytological material
immunoblotting*** (Western)
Working Dilution * 1:100 for immunohistochemistry
** 1:50 for paraffin-embedded tissue
*** 1:5000 - 1:10 000 for Western blotting (ECL)
Incubation Time 1 h at RT; extended with P
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide and 0.5% BSA
For research use only, not for therapeutic or diagnostic use!
References
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).
Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).
Guinea Pig Pab to Cytokeratin 8/18
Cat#RDI-PROGP11 $469.00
Category Guinea pig polyclonal (GP11)
Immunoglobulin Class IgG
Form/Purification Whole antiserum
Antigen Cytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18
Reactive Polypeptide Mr 52 500 and 45 500
(Specificity) (cytokeratins 8 and 18) of simple type epithelium
Antigen Recognized in Human, bovine, rat, mouse, Syrian hamster, Xenopus laevis
Tumors Specifically Detected All adeno-Ca tested, undifferentiated CA, hepato-cellular CA, cervix Ca.
Application Suitable for
immunohistochemistry on frozen* and paraffin-embedded**(P) tissue
cytological material
immunoblotting*** (Western)
ELISA
Working Dilution * 1:100 for immunohistochemistry
** 1:50 for paraffin-embedded tissue
*** 1:2000 for Western blotting
Incubation Time 1 h at RT; extended with P
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide
For research use only, not for therapeutic or diagnostic use!
References:
Rüther U. et al.: c-fos Expression interferes with thymus development in transgenic mice. Cell 53, 847-856 (1988).
Bader B.L. et al.: Low level expression of cytokeratins 8, 18 and 19 in vascular smooth muscle cells of human umbilical cord and in cultured cells derived therefrom, with an analysis of the chromosomal locus containing the cytokeratin 19 gene. Eur. J. Cell Biol. 47, 300-319 (1988).
Franke W.W. and Moll R.: Cytoskeletal components of lymphoid organs. Differentiation 36, 145-163 (1987).
Knapp A.C. and Franke W.W.: Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67-79 (1989).
Hacker H.J. et al.: Changes in the cellular phenotype and extracellular matrix during progression of estrogen-induced mesenchymal kidney tumors in Syrian hamsters. Virchows Arch. B Cell Pathol. 60, 213-223 (1991).
Guinea Pig Pab to Cytokeratin 9
Cat#RDI-PROGPCK9 $469.00
Source Guinea pig (GPHK9-TY1)
Form Stabilized antiserum
Antigen Synthetic peptide of human cytokeratin 9 (corresponding to aa. 450-477), coupled to KLH
Reactive Polypeptide (Specificity) Acidic human cytokeratin 9 (MW 62,129), expressed in the palmoplantar epidermis
Antigen Recognized in Human, bovine, mouse, rat
Species (tested so far)
Tissues Specifically Detected The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum. (see references listed below).
Application Suitable for immunohistochemistry on frozen* and paraffin-
embedded** (P) cells and tissue
immunoblotting*** (Western)
Working Dilution * 1:100-1:200 for immunohistochemistry
** 1:50 for paraffin-embedded tissue
*** 1:5000 - 1:10 000 for Western blotting (ECL)
Incubation Time 1 h at RT; extended with P
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide and 0.5% BSA
For research use only, not for therapeutic or diagnostic use!
References
1. Langbein L et al. Molecular characterization of the body site-specific human cytokeratin 9: cDNA cloning, amino acid sequence, and tissue specificity of gene expression. Differentiation 55:57-71 (1994)
2. Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)
Guinea Pig Pab to Cytokeratin 14
Cat#RDI-PROGPCK14 $470.00
Source Guinea pig (GP CK 14.2)
Form Stabilized antiserum
Antigen Recombinant human cytokeratin 14
Reactive Polypeptide (Specificity) Acidic cytokeratin14 (Mr 50 000), expressed in basal and
first suprabasal layers of epidermis
Antigen Recognized in Human, bovine, mouse
Species (tested so far)
Tumors Specifically Detected The antiserum reacts with cytokeratin 14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin 14 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references listed below).
Application Suitable for immunohistochemistry on frozen* and paraffin-
embedded** (P) tissue
cytological material
immunoblotting*** (Western)
Working Dilution * 1:100 - 1:200 for immunohistochemistry
** 1:50 for paraffin-embedded tissue
*** 1:5000 - 1:10 000 for Western blotting
Incubation Time 1 h at RT; extended with P
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide and 0.5% BSA
For research use only, not for therapeutic or diagnostic use!
References
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).
Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).
J Cell Science 113, 2471-2483 (2000)
J cell Biol 161, 1105-1115 (2003)
Eur J Cell Biol 82, 385-400 (2003)
Guinea Pig anti-cytokeratin 15
cat#RDI-PROGPCK15 $469.00/100ul
Source Guinea pig (GP CK 15.1)
Form whole antiserum
Antigen C terminal region (including parts of the rod domain) of recombinant human cytokeratin 1 5
Reactive Polypeptide
(Specificity) MW 50,000 0(pI4.9) intermediate filament polypeptide, cytokeratin 15, detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (eg bronchial epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicule, staining was restricted to the outermost cell layer of the outer root sheath; within the hair follicule several cell layers of the hair follicule were positive with antiserum gp15.1.
In human fetal tissues, cytokeratin 15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with antiserum gp15.1. The antiserum is completely unreactive with cells of liver, colon, urinary bladder, pancreas and lung alveolus.
In tumors, gp15.1 is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy (Heid, Bartek, Leube, Moll, Kaufmann & Franke, unpublished results)
Antigen Recognized in Human
Species (tested so far)
Application Suitable for immunohistochemistry on frozen sections
immunoblotting*** (Western)
Working Dilution * 1:100 - 1:200 for immunohistochemistry
*** 1:2000 Western blotting (ECL)
Incubation Time 1 h at RT
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide
For research use only, not for therapeutic or diagnostic use!
References
Heiid HW et al, in prep
Jih D.M. et al, J. Cutan Pathol 26, 113-118 (1999)
Guinea Pig Pab to Cytokeratin 17
Cat#RDI-PROGPCK17 $469.00
Form Stabilized antiserum
Antigen Recombinant human cytokeratin 17
Reactive Polypeptide (Specificity) Acidic human cytokeratin 17 (Mr 46,000)
Antigen Recognized in Human
Species (tested so far)
Tissues Specifically Detected The antiserum represents an excellent marker to distinguish myoepithelial cells from luminal epithelium of various glands (mammary, sweat, salivary, bronchial, tracheal, laryngeal, esophageal) and benign from malignant forms of e.g. mammary gland tumors.
Application Suitable for immunohistochemistry on frozen* and paraffin-embedded** (P) cells and tissue
immunoblotting*** (Western)
Working Dilution * 1:100-1:200 for immunohistochemistry
** 1:50 for paraffin-embedded tissue
*** 1:5000 - 1:10 000 for Western blotting (ECL)
Incubation Time 1 h at RT; extended with P
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide and 0.5% BSA
For research use only, not for therapeutic or diagnostic use!
References
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).
Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).
Guinea Pig Pab to Cytokeratin (PAN)
Cat#RDI-PROGP14 $469.00
Source Guinea pig
Form Whole antiserum
Antigen Cytoskeletal preparation of bovine snout epidermis
Reactive Polypeptide Most acidic and basic cytokeratins
(Specificity) (Mr 40000 - Mr 68000 polypeptides)
Antigen Recognized in Human, bovine, Xenopus, rat, mouse
Species (tested so far)
Tumors Specifically Detected All types of epithelia and epithelium-derived Neoplasms
Application Suitable for immunohistochemistry on frozen* and paraffin-
embedded** (P) tissue
cytological material
immunoblotting*** (Western)
Working Dilution * 1:100 for immunohistochemistry
** 1:50 for paraffin-embedded tissue
*** 1:2000 for Western blotting
Incubation Time 1 h at RT; extended with P
Storage At 2-8°C
Volume 100 µl; contains 0.09% sodium azide