rev:  December 15, 2004

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN 2E

see also clone KS 2.398.3.1  & RDI-PROGPCK2E


Mab to Cytokeratin 2e cat#RDI-PRO65191    $344.00/vial

Clone Determination        Ks 2.342.7.1

Category                           Mouse monoclonal

Immunoglobulin Class        IgG1

Purification                       Hybridoma culture supernatant

Antigen                              Synthetic peptide (N-terminal amino acids Nos. 2-23) of human cytokeratin 2e (MW 65,852)

Specificity                        Ks2.342.7.1 represents an excellent marker to study terminal epidermal differentiation. The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). It is also reactive on hyperkeratosis of diverse viral and genetic origin.  The mab does not react with palate cytokeratin 2p.

Antigen recognized  in species (tested so far) Human,. bovine, calf hoof epidermis, snooze epithelium; negative with tongue epithelium; mouse/rat (heterogenously on foot sole epidermis only, negative with epidermis of other body sites.

Application Suitable for · Immunoblotting (Western)*

                                         · Immunohistochemistry  on frozen tissue and paraffin-embedded sections**

Working Dilution * 1:200 (alkaline phosphatase)

                            * 1:60000 (chemiluminescence)

                            ** 1:200 (immunofluorescence)

                            ** 1:100 (immunohistochemistry PAP/ABC)

Storage At 4°C

Volume 1 ml

References

1. Collin C., Moll R., Kubicka S., Ouhayoun J.-P. and Franke W.W. (1992). Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation, Exp. Cell Res. 202: 132-141.

2. Langbein L., Moll I., Prätzel S., Moll R., Franke W.W. Specific expression of cytokeratin 9 and 2e in cutaneous disorders of variant body sites (manuscript in preparation).

3. Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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