rev: 5/15/2008
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CYTOKERATIN ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN 2E
see also clone KS 2.398.3.1 & RDI-PROGPCK2E
Mab to Cytokeratin 2e
cat#RDI-PRO65191, new cat#: 10R-C166a $379.50/1ml
Clone Determination Ks 2.342.7.1
Category Mouse monoclonal
Immunoglobulin Class IgG1
Purification Hybridoma culture supernatant
Antigen Synthetic peptide (N-terminal amino acids Nos. 2-23) of human cytokeratin 2e (MW 65,852)
Specificity Ks2.342.7.1 represents an excellent marker to study terminal epidermal differentiation. The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). It is also reactive on hyperkeratosis of diverse viral and genetic origin. The mab does not react with palate cytokeratin 2p.
Antigen recognized in species (tested so far) Human,. bovine, calf hoof epidermis, snooze epithelium; negative with tongue epithelium; mouse/rat (heterogenously on foot sole epidermis only, negative with epidermis of other body sites.
Application Suitable for · Immunoblotting (Western)*
· Immunohistochemistry on frozen tissue and paraffin-embedded sections**
Working Dilution * 1:200 (alkaline phosphatase)
* 1:60000 (chemiluminescence)
** 1:200 (immunofluorescence)
** 1:100 (immunohistochemistry PAP/ABC)
Storage At 4°C
Volume 1 ml
References
1. Collin C., Moll R., Kubicka S., Ouhayoun J.-P. and Franke W.W. (1992). Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation, Exp. Cell Res. 202: 132-141.
2. Langbein L.,
3. Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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