rev: 5/15/2008
HOME (index page)
Return (alphabetical antibody page)
Return (cytokeratin data page)
CYTOKERATIN ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN 6
Mab to Cytokeratin 6
cat#RDI-PRO65190, new cat#: 10R-C172b $379.50/50ug
Clone
Ks6.KA12
Category
Mouse monoclonal
Ig Subclass
IgG1
Purification/Form
Hybridoma culture supernatant (contains 0.1% sodium azide)
Antigen
Cytokeratin 6 of human
callus cytoskeletal preparation
Clinical Application/
Specificity
Ks6.KA12 represents an excellent
marker for non-keratinized squamous epithelia and
proliferating cells of epidermis (e.g. within
psoriatic lesions).
Application Suitable for· immunohistochemistry on frozen tissue and
paraffin-embedded material (P) after formalin- fixation; microwave treatment
improves performance· immunoblotting (Western blot)
Storage/Stability at 4°C (undiluted) for min 1 year
Volume 5 ml
Polypeptide Reacting Mr 56 000 polypeptide (cytokeratin 6) of human squamous
epithelia
Antigen Recognized in Species (tested so far) Human , mouse (see below
cat#RDI-PRO61090, new cat#: 10R-C172a)
Reactivities Cell Lines (tested so far) : Cultured A-431, Detroit
562, SCC-12
Tissues and Tumors Specifically Detectedor: The antibody is suitable for
discrimination of keratinizing and non-keratinizing squamous cell carcinoma
versus e.g. poorly differentiated adenocarcinoma.
Working Dilution Ready-to-use for immunohistochemistry
Incubation Time 1 h at RT, extended with (P) for immu-nohistochemical
application
References
Smedts F et al. Keratin expression in cervical cancer.
Am J Pathol 141:497-511 (1992)
Wetzels RHW et al. Basal cell-specific and hyperproliferation-related keratins
in human breast cancer. Am J Pathol 138:751-763 (1991)
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in
adnexal neoplasms of the skin: an immunohistochemical study including
individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).
Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren:
Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der
Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).
For research use only. Not supplied for use in human
diagnostic or therapeutic procedures
Mab to Cytokeratin 6
cat#RDI-PRO61090, new cat#: 10R-C172a $330.00/vial 50ug
(same clone as cat#RDI-PRO65190 , now purified)
Clone Ks6 .KA12
Category Mouse monoclonal
Ig Subclass IgG1
Purification/Form A affinity chromatography
Antigen Cytokeratin 6 of human callus cytoskeletal preparation
Clinical Application/Specificity: Ks6.KA12 represents an excellent marker for non-.
keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).
Application Suitable for
· immunohistochemistry on
frozen tissue and paraffin-embedded material (P) after formalin- fixation;
microwave treatment improves performance· immunoblotting (Western blot)
Storage/Stability
at 2-8°C (undiluted) for min 1 year
Volume
50 micrograms lyophilized
(contain 0.5% BSA and 0.09% NaN3)
Polypeptide Reacting Mr 56 000 polypeptide (cytokeratin 6) of human squamous epithelia
Antigen Recognized in Species (tested so far) Human mouse+
Reactivities on Cultured A-431,
Cell Lines (tested so far)
Tissues and Tumors specifically detected:
The antibody is suitable for discrimina of keratinizing and non-keratinizing
squamous cell carcinoma versus e.g. poorly differentiated adenocarcinoma.
Working Dilution econstitute with 1ml DI water, use approx 1:10 dilution with
PBS for immunohisto-chemistry
Incubation Time 1 h at RT, extended with (P) for immuno-histochemical
application
References
Smedts F et al. Keratin expression in cervical cancer. Am J Pathol 141:497-511 (1992)
Wetzels RHW et al. Basal cell-specific and hyperproliferation-related keratins in human breast cancer. Am J Pathol 138:751-763 (1991)
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).
Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).
+ Herrman T et al , Mice with targeted disruption of the fatty acid transport protein 4 (FATp 4, SIc27a4) gene show features of lethal restrictive dermopathy. J Cell Biol 161, 1105-1115 (2003)
For In Vitro Research Use Only
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
RETURN(cytokeratin page)
Return (alphabetical antibody page)