rev:January 10, 1997
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYLICIN I
Mab to Cylicin I (a basic protein of the sperm head cytoskeleton)
cat#RDI-PRO65142 $281.00/vial
Clone Determination
Cal-144 (X 144.3.2)
Category
Mouse monoclonal
Immunoglobulin Subclass IgM
Purification
Ascites or hybridoma culture supernatant
Antigen Calyx preparation from bovine sperm (according to Longo et al. 1987, JCB 22, 1105-1120)
Specificity Especially useful in discrimination of morphologically altered "round-headed" spermatozoa
Application Immunofluorescence microscopy
Immunoblotting (Western)
Polypeptide(s) Reacting Bovine cylicin I (Mr 74.788, deduced from sequence analysis),
a basic protein (IEP 10.55) of the sperm head cytoskeleton with
Mr 58.000 on SDS-PAGE; Multiple band proteins with MW 58,
63, 69 and 74 kDa of calyx-associated polypeptides of bovine
spermatozoa;
Antigen Recognized in Human, bovine
Species (tested so far)
Working Dilution Ready-to-use for immunofluorescence
Incubation Time 1 h at RT
Storage At 4°C
Volume 5 ml
References
Hess, H., Heid, H. and Franke, W.W.: Molecular Characterization of Mammalian Cylicin, a Basic Protein of the Sperm Head Cytoskeleton. J. Cell Biol. 5, 1043-1052 (1993)
For research use only. Not supplied for use in human diagnostic or therapeutic
procedures
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (800) 370-2222 or (201) 584-7093
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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