rev" May 1, 2001

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN 8

Mab to Cytokeratin 8

cat#RDI-PRO65130                  $219.00/vial

Clone Determination                  Ks 8-17.2

Category                                     Mouse monoclonal

Immunoglobulin Class                 IgG1

Purification                                  Hybridoma culture supernatant

Antigen                                         Cytoskeletal preparation from human tumor cell line MCF-7

Specificity                                   Ks 8-17.2 represents an excellent marker to discriminate simple epithelia from those of different origin.

Application                                 Suitable for frozen tissue  Cytological material

                                                   Immunoblotting (Western

Storage                                       At 2-8 DEG C

Quantity                                      5ml TCS; contains 0.5% BSA and 0.09% NaN3

Polypeptide Reacting                Mr 52 500 polypeptide (cytokeratin No. 8) of human epithelia

Antigen Recognized in                Human, rat, bovine, hamster

Species (tested so far)

Reactivities on Cultured Cell lines: MCF-7, HeLa, A-431, PLC, Detroit 562

Tumors detected:                       All adeno-Ca tested; undifferentiated CA, cervix CA, hepatocellular CA

Working Dilution                      Ready for use in immunohistochemistry

 Incubation Time                      1 h at RT

References

Heid, H er al, Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentitaion (2000) submitted

Magin, T.M. et al, Eur J Cell Biol 53, 333-348 (1990)

Jaohn; L et al, Differentiation 40:55-2 (1989(

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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