rev: March 17, 2004
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
RDI-PRO65127 $250.00
Clone Determination Ks 8.10 (=10B10)
Category Mouse monoclonal
Immunoglobulin Class IgG 1
Purification Hybridoma culture supernatant
Antigen Cytoskeletal proteins from cultured MCF-7 cells
Specificity Ks 18.10 represents an excellent marker to discriminate simple epithelia from those of d ifferent origin
Application Suitable for frozen tissue
Cytological material
Immunoblotting (Western)
ELISA
Polypeptide Reacting MR 52 500 polypeptide (cytokeratin
8) of all simple type and complex epithelia
Antigen Recognized in Human, mouse, rat, tupaia belangeri, monodelphis domestica;
negative with bovine, pig, chicken, Xenopus laevis
Reactivities on Cultured MCF-7, PLC, RT 112
Cell Lines (tested so far)
Tumors Specifically
Detected All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA
Working Dilution Ready-to-use for immunohistochemistry
Incubation Time 1 h at RT
Storage At 2-8°C
Volume 5 ml
References
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)
Osborn, M., van Lessen, G., Weber, K., Klöppel, G. and Altmannsberger, M.: Methods in Laboratory Investigation. Differential diagnosis of gastrointestinal carcinomas by using monoclonal antibodies specific for individual keratin polypeptides. Lab Investigation 55, 497-504 (1986)
Cat. No RDI-PRO65127
For Research Use Only
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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