rev: December 8, 2004
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Cat#RDI-PRO61808 $438.00
Clone Determination K8.60
Category Mouse monoclonal
Immunoglobulin Class IgG1
Purification Ascites fluid
Antigen Bovine epidermal keratins
Specificity K8.60 represents a marker for cornification, which may indicate stage and degree of squamous CA differentiation.The antibody mainly reacts with cytokeratins 10 and 11, low reactivity is obtained with cytokeratin 1. K8.60 stains suprabasal layers of epidermis as well as individual cells in other squamous epithelia.
Application Suitable for
· · frozen and paraffin-embedded tissue (P)
· cytological material
· immunoblotting (Western)
Storage At 2-8° C
Polypeptide Reacting MW 68 000, MW 56 500 and MW 56 000 polypeptides
(cytokeratin 1, 10 and 11) of human epidermis
Antigen Recognized in Human, bovine
Species (tested so far)
Reactives in Cultured Not tested Cell Lines (tested so far)
Tumors Specifically Detected Squamous cell CA of various origins
Pretreatment With paraffin-embedded-sections, protease Pretreatment is required prior to antibody application
Working Dilution Dilute 1:20 for use in immunohistochemistry
Incubation Time 1h at RT; extended with paraffin-embedded sections
Quantity 500 µl
References
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)
Huszar, M., Gigi-Leitner, O., Moll, R., Franke, W.W. and Geiger, B.: Monoclonal antibodies to various acidic (type I) cytokeratins of stratified epithelia: Selective markers for stratification and squamous cell carcinomas. Differentiation 31, 141-153 (1986)
Whittaker, J.R., Samy, A.M.J., Sunter, J.P., Sinha, D.P. and Monaghan, J.M.: Cytokeratin expression in cervical epithelium: An immunohistological study of normal, wart virus-infected and neoplastic tissue. Histopathology 14, 151-160 (1989)
For In Vitro Research Use Only
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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