rev:      July 5, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat#RDI-PRO61075                 $375.00/vial

Clone Determination               Ac1-20.4.2

Category                                  Mouse monoclonal

Immunoglobulin                       Class IgG1

Purification                              Protein G affinity chromatography

Antigen                                     Synthetic NH2 terminus decapeptide of cardiac isoform of actin

Specificity                                Ac1 represents an excellent marker for cardiac tissue; it discriminates cardiac actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross-reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied (see under Special Treatment*)

Application · Immunoblotting (Western)

                      · ELISA

                     · Immunohistochemistry on frozen sections and paraffin-embedded tissue after microwave treatment

Stability At 4°C one year after reconstitution

Quantity 50 µg (lyoph.) contains 0.5% BSA and 0.09% NaN3

The monoclonal antibody to cardiac / fetal actin (cat. no.RDI-PRO61075) is specific for cardiac actin, both in the fetal and the adult heart. Additionally to myocardial localization the antibody detects fetal actin in regenarating muscle (e.g. after injury) and in certain types of stem cells.

The monoclonal antibody to cardiac / fetal actin has been positively tested on human, bovine, rabbit, and chicken tissue. However, from amino acid sequence data it is known that the reactive epitope is also present on the fetal actin polypeptide of e.g. rat, mouse, trout, and japanese pufferfish. Accordingly, a positive cross-reaction can be expected.

Special Treatment IT is necessary to include 0.5 M NaCl in all washing buffers for application on frozen tissue to enhance specificity and 1M NaCl for application in Western blotting and Elisa to enhance specificity. This is not necessary for paraffin sections.

Working Dilution When reconstituted with 1 ml distilled water, dilute further

1:10 for immunohistochemistry

1:1000 for immunoblotting (using the ECL-enhanced procedure)

1:500 for ELISA

Incubation Time 1 h at RT

References

1. Vandekerckhove, J. and Weber, K. (1979). The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. Differentiation 14, 123-133.

2. Franke, W.W., Stehr, S., Stumpp, S., Kuhn, C., Heid, H., Rackwitz, H.-R., Schnölzer, M., Baumann, R., Holzhausen, H.-J. and Moll, R. (1996). Specific immunohistochemical detection of cardiac/fetal a-actin in human cardiomyocytes and regenerating skeletal muscle cells. Differentiation 60, 245-250.

3. Rudy DE, Yatskievych TA, Antin PB and Gregorio CC (2001). Assembly of thick, thin, and Titin filaments in chick precardiac explants. Developmental Dynamics 221, 61-71.

4> Ehler et al (2004) Myofibrillogenesis int he developing chicken heart:Role of actin isoforms and of pointed end actin capping protein tropomodulin during thin filmament assemby. Developmental Dynamics 229, 745-755 (2004)

For Research Use only

a) (monoclonal antibody to cardiac actin) reacts with cardiac / fetal actin which has an apparent molecular weight of 43 kD (like sarcomeric actin). Depending on the gel system used before transfer to the blotting membrane (Western blotting) it might range from 40 to 45 kD.

The monoclonal antibody to cardiac / fetal actin (cat. no. 61075) is specific for cardiac actin, both in the fetal and the adult heart. Additionally to myocardial localization the antibody detects fetal actin in regenarating muscle (e.g. after injury) and in certain types of stem cells. The monoclonal antibody to cardiac / fetal actin has been positively tested on human, bovine, rabbit, and chicken tissue. However, from amino acid sequence data it is known that the reactive epitope is also present on the fetal actin polypeptide of e.g. rat, mouse, trout, and japanese pufferfish. Accordingly, a positive cross-reaction can be expected.

-see also pure cardiac actin protein cat#RDI-PRO62224  $438.00/100ug

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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