rev:  Febraury 21, 2002

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN  20

cat#RDI-PRO61054    $406.00/vial

-also available FITC conjugated cat#RDI-PRO61454   $438.00/0.25ml (approx 50ug)

Clone Determination IT-Ks 20.10

Category Mouse monoclonal

Immunoglobulin Class IgG1

Purification SpAss-Protein A (recombinant)

Antigen Electrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa

Clinical Value IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin.

Application Suitable for frozen tissue, paraffin-embedded sections only after microwave treatment

                   Immunoblotting (Western)

Stability/Storage at 4°C one year after reconstitution

Quantity 50 µg (lyoph.)

Polypeptide Reacting Protein IT (cytokeratin No. 20; Mr 46 000)

Antigen Recognized in Species tested so far: Human, pig, mouse, rat

Reactivities on Cultured Cell lines tested so far:HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4

Tissues/Tumors Specifically Detected Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal CA). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially

Working Dilution Reconstitute in 1 ml dist. water and dilute 1:10 in PBS (pH 7.4) for immunohistochemical application.

Incubation Time 1 h at RT or over night at 4°C.

Literature on Cytokeratin 20

Moll, R., Löwe, A., Laufer, J. and Franke, W.W.: Cytokeratin 20 in human carcinomas: A new histodiagnostic marker detected by monoclonal antibodies. Am.J.Path. 140, 427-447 (1992)

Moll, R., Schiller, D.L. and Franke, W.W.: Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J.Cell.Biol. 111, 567-580 (1990)

Moll, I. and Moll, R.: Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res. 283, 300-309 (1991)

Moll, R., Zimbelmann, R., Goldschmidt, M.D., Keith, M., Laufer, J., Kasper, M., Koch, P.J., Franke, W.W.: The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation 53, 75-93 (1993)

V.J. Möbus, R. Moll, C.D. Gerharz, D.G. Kieback, W. Weikel, G. Hoffmann & R. Kreienberg: Establishment of new ovarian and colon carcinoma cell lines: differentiation is only by cytokeratin analysis. Br. J. Cancer 69, 422-428 (1994)

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

-see also rec human cytokeratin 20 antigen

- TUMORS -

Miettinen Mod Pathol 1995 Marker for gastrointestinal, urothelial, and Merkel cell carcinomas (788 cases)

Wang et al. Appl Immunohistochem 1995 Marker for colon, stomach, pancreas and urothelial carcinomas

Wauters et al. Hum Pathol 1995 Marker for colon and gastric cancer metastatic to the ovary

Burchill et al. Br J Cancer 1995 Marker for colon carcinoma cells (RT-PCR)

Ascoli et al. Diagn Cytopathol 1995 Marker for gastrointestinal carcinoma cells

Loy + Calaluce Am J Clin Pathol 1994 Marker for colon carcinomas (versus pulmonary adenocarcinomas)

Sarbia et al. Pathol Res Pract 1993 Marker for Barrett´s carcinomas (esophagus)

Betke et al. J Am Acad Dermatol 1993 Marker for gastric carcinomas (skin metastasis)

Suo et al. Histopathology 1993 Negative marker for squamous cell carciniomas

Smedts et al. Am J Pathol 1993 Negative marker for squamous cell carcinomas

CYTOKERATIN 20 IN THE LITERATURE

- NORMAL CELLS -

Flint et al. Epithelial Cell Biol 1994 Marker for intestinal epithelial cells of villi

Narisawa+Kohda J Cutan Pathol 1995 Marker for Merkel cells

Kim + Holbrook J. Invest Dermatol 1995 Marker for Merkel cells

Bouwens et al. Diabetes 1994 Marker for rat pancreatic duct cells

Bouwens et al. J Histochem Cytochem 1995 Marker for rat pancreatic duct cells

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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